河南科技
河南科技
하남과기
Journal of Henan Science and Technology
2015年
11期
113-115
,共3页
荧光光谱法%紫外光谱法%丽春红S%牛血清白蛋白%荧光偏振%荧光猝灭作用
熒光光譜法%紫外光譜法%麗春紅S%牛血清白蛋白%熒光偏振%熒光猝滅作用
형광광보법%자외광보법%려춘홍S%우혈청백단백%형광편진%형광졸멸작용
fluorescence spectroscopy%UV spectrophotometry%Ponceau S%bovine serum albumin%fluorescence polar?ization%fluorescence quenching
采用荧光偏振技术,运用荧光光谱法、紫外光谱法和三维荧光光谱法研究了在pH=7的PBS缓冲介质中,不同浓度的丽春红S(Ponceau S)和一定量牛血清白蛋白(bovine serum albumin,BSA,以下简称 BSA)的光谱作用.荧光光谱表明随着染料浓度的增加,荧光偏振强度(FP)呈下降的趋势,其关系式为:F0/F =0.0011C+1.1435,R=0.9938,说明丽春红S与BSA相互作用产生了荧光猝灭;紫外光谱表明,随着染料浓度的增加,吸光度呈增大的趋势.
採用熒光偏振技術,運用熒光光譜法、紫外光譜法和三維熒光光譜法研究瞭在pH=7的PBS緩遲介質中,不同濃度的麗春紅S(Ponceau S)和一定量牛血清白蛋白(bovine serum albumin,BSA,以下簡稱 BSA)的光譜作用.熒光光譜錶明隨著染料濃度的增加,熒光偏振彊度(FP)呈下降的趨勢,其關繫式為:F0/F =0.0011C+1.1435,R=0.9938,說明麗春紅S與BSA相互作用產生瞭熒光猝滅;紫外光譜錶明,隨著染料濃度的增加,吸光度呈增大的趨勢.
채용형광편진기술,운용형광광보법、자외광보법화삼유형광광보법연구료재pH=7적PBS완충개질중,불동농도적려춘홍S(Ponceau S)화일정량우혈청백단백(bovine serum albumin,BSA,이하간칭 BSA)적광보작용.형광광보표명수착염료농도적증가,형광편진강도(FP)정하강적추세,기관계식위:F0/F =0.0011C+1.1435,R=0.9938,설명려춘홍S여BSA상호작용산생료형광졸멸;자외광보표명,수착염료농도적증가,흡광도정증대적추세.
The spectral effects of S(S Ponceau)with different concentration and serum bovine(BSA)with certain amount in the buffer medium of PBS with pH=7 were studied by fluorescence polarization technique,fluorescence spectroscopy,UV spectroscopy and three-dimensional fluorescence spectroscopy. Fluorescence spectra showed that the intensity of fluorescence polarization(FP)decreased with the increase of dye concentration,and the relationship was:=0.0011C+1.1435 R=0.9938,F0/F. That meant fluorescence quenching was produced by the interaction of Pon?ceau S and bovine serum albumin(BSA);UV spectra showed that with the increase of the dye concentration,absor?bance had a roughly increasing trend.
