中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
9期
2163-2165
,共3页
张自森%王世超%夏兴洲%吴诗文%吴万庆%傅聿铭
張自森%王世超%夏興洲%吳詩文%吳萬慶%傅聿銘
장자삼%왕세초%하흥주%오시문%오만경%부율명
奥曲肽%胃癌%多聚ADP核糖聚合酶1%细胞增殖核抗原
奧麯肽%胃癌%多聚ADP覈糖聚閤酶1%細胞增殖覈抗原
오곡태%위암%다취ADP핵당취합매1%세포증식핵항원
Octreotide%Gastric carcinoma%Poly adenosine diphosphate-ribose polymerase-1%Proliferating cell nuclear antigen
目的 观察奥曲肽对胃癌AGS细胞多聚ADP核糖聚合酶1(PARP-1)、细胞增殖核抗原(PCNA)、细胞周期蛋白D1 (Cyclin D1)表达的影响及其作用.方法 利用不同浓度的奥曲肽(0、0.001、0.010、0.100、1.000、10.000 mg/L)作用于胃癌AGS细胞,采用Western blot方法分析PARP-1、PCNA和Cyclin Dl蛋白相对表达变化,细胞计数试剂盒(CCK-8)法检测胃癌AGS细胞增殖抑制,流式细胞术检测胃癌AGS细胞凋亡和细胞周期变化.结果 随奥曲肽浓度的增加,胃癌AGS细胞中PARP-1蛋白的表达逐渐增强(0.08、0.09、0.12、0.16、0.21、0.22),PCNA蛋白的表达逐渐减弱(0.05、0.04、0.02、0.01、0.01、0.0l),Cyclin D1蛋白表达无显著变化(0.44、0.40、0.39、0.40、0.43、0.40,P＞0.05),PARP-1和PCNA蛋白表达呈负相关(P＜0.05).奥曲肽在0～0.100 mg/L浓度范围内,对胃癌AGS细胞增殖抑制逐渐增强,高于0.100 mg/L浓度后,抑制作用逐渐减弱.各浓度奥曲肽作用下,胃癌AGS细胞凋亡和各周期细胞比例未见显著变化(P＞0.05).结论 奥曲肽浓度可对胃癌AGS细胞增殖产生双向影响作用,其机制可能与PARP-1蛋白表达增强和PCNA蛋白表达抑制有关.
目的 觀察奧麯肽對胃癌AGS細胞多聚ADP覈糖聚閤酶1(PARP-1)、細胞增殖覈抗原(PCNA)、細胞週期蛋白D1 (Cyclin D1)錶達的影響及其作用.方法 利用不同濃度的奧麯肽(0、0.001、0.010、0.100、1.000、10.000 mg/L)作用于胃癌AGS細胞,採用Western blot方法分析PARP-1、PCNA和Cyclin Dl蛋白相對錶達變化,細胞計數試劑盒(CCK-8)法檢測胃癌AGS細胞增殖抑製,流式細胞術檢測胃癌AGS細胞凋亡和細胞週期變化.結果 隨奧麯肽濃度的增加,胃癌AGS細胞中PARP-1蛋白的錶達逐漸增彊(0.08、0.09、0.12、0.16、0.21、0.22),PCNA蛋白的錶達逐漸減弱(0.05、0.04、0.02、0.01、0.01、0.0l),Cyclin D1蛋白錶達無顯著變化(0.44、0.40、0.39、0.40、0.43、0.40,P＞0.05),PARP-1和PCNA蛋白錶達呈負相關(P＜0.05).奧麯肽在0～0.100 mg/L濃度範圍內,對胃癌AGS細胞增殖抑製逐漸增彊,高于0.100 mg/L濃度後,抑製作用逐漸減弱.各濃度奧麯肽作用下,胃癌AGS細胞凋亡和各週期細胞比例未見顯著變化(P＞0.05).結論 奧麯肽濃度可對胃癌AGS細胞增殖產生雙嚮影響作用,其機製可能與PARP-1蛋白錶達增彊和PCNA蛋白錶達抑製有關.
목적 관찰오곡태대위암AGS세포다취ADP핵당취합매1(PARP-1)、세포증식핵항원(PCNA)、세포주기단백D1 (Cyclin D1)표체적영향급기작용.방법 이용불동농도적오곡태(0、0.001、0.010、0.100、1.000、10.000 mg/L)작용우위암AGS세포,채용Western blot방법분석PARP-1、PCNA화Cyclin Dl단백상대표체변화,세포계수시제합(CCK-8)법검측위암AGS세포증식억제,류식세포술검측위암AGS세포조망화세포주기변화.결과 수오곡태농도적증가,위암AGS세포중PARP-1단백적표체축점증강(0.08、0.09、0.12、0.16、0.21、0.22),PCNA단백적표체축점감약(0.05、0.04、0.02、0.01、0.01、0.0l),Cyclin D1단백표체무현저변화(0.44、0.40、0.39、0.40、0.43、0.40,P＞0.05),PARP-1화PCNA단백표체정부상관(P＜0.05).오곡태재0～0.100 mg/L농도범위내,대위암AGS세포증식억제축점증강,고우0.100 mg/L농도후,억제작용축점감약.각농도오곡태작용하,위암AGS세포조망화각주기세포비례미견현저변화(P＞0.05).결론 오곡태농도가대위암AGS세포증식산생쌍향영향작용,기궤제가능여PARP-1단백표체증강화PCNA단백표체억제유관.
Objective To investigate the effect of octreotide on the expression of poly adenosine diphosphate-ribose polymerase (PAR P)-1 and proliferating cell nuclear antigen (PCNA) in human gastric carcinoma cell line AGS cells and the possible mechanism.Methods AGS cells were cultured in vitro,and treated by octreotide with different concentrations of 0,0.001,0.010,0.100,1.000,10.000 mg/L.The expression of PARP-1,PCNA and Cyclin D1 was detected by Western blotting.The growth inhibition rate of AGS cells was determined by cell counting Kit-8 (CCK-8) method.The apoptosis rate and cell cycle ratio were analyzed by flow cytometry.Results The expression level of PARP-1 protein was increased (0.08,0.09,0.12,0.16,0.21,0.22) and PCNA protein decreased (0.05,0.04,0.02,0.01,0.01,0.01) with the increased concentration of octreotide (P ＜ 0.05).There was no significant variation in the Cyclin D1 expression among the concentrations of octreotide (P ＞ 0.05).The inhibition rate of AGS cells by octreotide was increased gradually between the concentration of 0-0.100 mg/L,on the contrary,that was decreased sharply when the concentration of octreotide over 0.100 mg/L.No significant difference in apoptosis rate and cell cycle ratio was observed in AGS cells treated by octreotide with different concentrations (P ＞ 0.05).Conclusion Different concentrations of octreotide could develop bidirectional effect on proliferation of AGS cells,which might be correlated with PARP-1 up-regulation and PCNA down-regulation.
