CAJ | 학술논문

目的建立SK-BR-3和MDA-MB-468裸鼠皮下种植瘤模型,观察两种乳腺癌细胞株在建立裸鼠皮下移植瘤中的成瘤性和生物学与形态学特征.探讨阿霉素缓释剂对两种乳腺癌细胞裸鼠皮下种植瘤的生物学功能的影响.方法将80只裸小鼠随机分为MDA-MB-468组和SK-BR-3组,MDA-MB-468组小鼠皮下注射乳腺癌MDA-MB-468细胞株,SK-BR-3组小鼠皮下注射乳腺癌SK-BR-3细胞株,每组40只.选取一半数量的小鼠,采用皮下注射法将细胞种植在裸鼠右侧腹股沟区,每天注射1次,连续注射4周后处死裸鼠,剥离瘤体,计算两组裸鼠皮下肿瘤的体积抑瘤率,取出两组小鼠模型中另一半小鼠,将阿霉素缓制剂注入两组小鼠模型皮下肿瘤旁,4周后处死两组小鼠,剥离瘤体,测量肿瘤大小.采用原位缺口末端标记法(TUNEL)法检测两组小鼠瘤体组织中肿瘤细胞的凋亡指数,观察阿霉素对两组乳腺癌细胞周期和凋亡的影响.结果 (1)肿瘤生长:至第8周时,两组小鼠肿瘤的体积分别为(3.212±0.151)和(2.924 ±0.316) cm3,两组间差异有统计学意义(t=2.758,P＜0.05).(2)注射阿霉素后肿瘤体积抑制率结果:两组乳腺癌小鼠模型注射阿霉素缓释剂后,皮下瘤体停止生长并逐渐缩小,两组肿瘤体积差异有统计学意义(x2=2.53,P＜0.05).(3)阿霉素对两组细胞周期和凋亡的影响:阿霉素使两组细胞生长阻滞在G0/G1期.阿霉素可显著引起MDA-MB-468细胞凋亡,最大凋亡率达62.5％.阿霉素处理SK-BR-3细胞后,亦可引起较明显的细胞凋亡,最大凋亡率可达53.8％.结论成功制备两种乳腺癌转移瘤小鼠模型,同时制备阿霉素缓释剂,证实了其在乳腺癌转移瘤小鼠模型中抑制肿瘤生长的良好疗效.
목적 건립SK-BR-3화MDA-MB-468라서피하충식류모형,관찰량충유선암세포주재건립라서피하이식류중적성류성화생물학여형태학특정.탐토아매소완석제대량충유선암세포라서피하충식류적생물학공능적영향.방법 장80지라소서수궤분위MDA-MB-468조화SK-BR-3조,MDA-MB-468조소서피하주사유선암MDA-MB-468세포주,SK-BR-3조소서피하주사유선암SK-BR-3세포주,매조40지.선취일반수량적소서,채용피하주사법장세포충식재라서우측복고구구,매천주사1차,련속주사4주후처사라서,박리류체,계산량조라서피하종류적체적억류솔,취출량조소서모형중령일반소서,장아매소완제제주입량조소서모형피하종류방,4주후처사량조소서,박리류체,측량종류대소.채용원위결구말단표기법(TUNEL)법검측량조소서류체조직중종류세포적조망지수,관찰아매소대량조유선암세포주기화조망적영향.결과 (1)종류생장:지제8주시,량조소서종류적체적분별위(3.212±0.151)화(2.924 ±0.316) cm3,량조간차이유통계학의의(t=2.758,P＜0.05).(2)주사아매소후종류체적억제솔결과:량조유선암소서모형주사아매소완석제후,피하류체정지생장병축점축소,량조종류체적차이유통계학의의(x2=2.53,P＜0.05).(3)아매소대량조세포주기화조망적영향:아매소사량조세포생장조체재G0/G1기.아매소가현저인기MDA-MB-468세포조망,최대조망솔체62.5％.아매소처리SK-BR-3세포후,역가인기교명현적세포조망,최대조망솔가체53.8％.결론 성공제비량충유선암전이류소서모형,동시제비아매소완석제,증실료기재유선암전이류소서모형중억제종류생장적량호료효.
Objective To establish the tumor models of SK-BR-3 and MDA-MB-468 in subcutaneous xenograft,observe the two breast cancer cell lines in the establishment of tumor and the biological and morphological characteristics in to the subcutaneous transplantation tumor in nude mice.To judge the impact of biological function of adriamycin on two breast cancer cell subcutaneous implanted tumor in nude mice.Methods 80 nude mice were randomly divided into group MDA-MB-468 and group SK-BR-3,group MDA-MB-468 mice subcutaneous injection of breast cancer cells MDA-MB-468,SK-BR-3 mice by subcutaneous injection of breast cancer cell line SK-BR-3,with 40 rats in each group.Selection of half the number of mice by subcutaneous injection of the cells grown in nude mice right groin area,1 daily injections for 4 weeks after injection,mice were sacrificed and the tumor body.Two groups of nude mice tumor volume measurement,calculation of tumor volume inhibition rate,remove the other half mice in two groups in a mouse model of adriamycin,will slow preparation into two groups of mice subcutaneous tumor model by injection,daily 1 times,continuous injection for 4 weeks.Observation of two nude mice,the growth of the dietandmental status,tumor record,mice were killed after 4 weeks,the tumor volume,the tumor size was measured.The apoptosis index of tumor cells was detected by TdT-mediated dUTP nick end labeling (TUNEL) in two groups of mice tumor tissue,to observe the effect of adriamycin on the two groups of breast cancer cell cycle and apoptosis.Results (1) Tumor growth:at eighth weeks,two groups of mice tumor volume were (3.212 ±0.151) and (2.924 ±0.316) cm3,the difference was statistically significant between the two groups (t =2.758,P ＜ 0.05).Results the tumor volume inhibition rate.(2) After adriamycin injection:two groups of mouse model of breast cancer after injection of adriamycin,subcutaneous tumors stopped growing and gradually narrowing,4 weeks later,the tumor volume was statistically significant difference between the two groups of mice (x2 =2.14,P ＜ 0.05),detection index of tumor cell apoptosis by TUNEL,there was statistical significance the difference between the two groups (x2 =3.59,P ＜0.05).(3) Effect of adriamycin on cell cycle and apoptosis of two groups:the two group of adriamycin induced cell growth arrest at G0/G1 phase.After treatment of MDA-MB-468 cells with doxorubicin,MDA-MB-468 cells with sub diploid peak before G1 phase peak,showed that adriamycin can induce MDA-MB-468 cells apoptosis,the apoptosis rate was 62.5％,the maximum.After treatment of SK-BR-3 cells with doxorubicin,also can cause cell apoptosis obviously,the maximum apoptosis rate was up to 53.8％.Conclusion We successfully prepared two kinds of breast cancer metastasis mouse model,at the same time,the preparation of sustained-release adriamycin,confirmed its good effect of growth inhibition of tumor metastasis in a mouse model of breast cancer,has important clinical significance.