中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
9期
2054-2058
,共5页
王林%代兴亮%王海洋%王中勇%王爱东%董军%兰青%黄强
王林%代興亮%王海洋%王中勇%王愛東%董軍%蘭青%黃彊
왕림%대흥량%왕해양%왕중용%왕애동%동군%란청%황강
胶质瘤干细胞%荧光示踪%细胞融合%细胞社会%活细胞缩时摄影
膠質瘤榦細胞%熒光示蹤%細胞融閤%細胞社會%活細胞縮時攝影
효질류간세포%형광시종%세포융합%세포사회%활세포축시섭영
Glioma stem cells%Dual fluorescence tracing method%Cell fusion%Cell sociology%Live cell time-lapse shoot
目的 通过分析免疫细胞社会中的肿瘤细胞与宿主巨噬细胞之间的关系,探寻肿瘤细胞社会中打破免疫平衡的大事件.方法 共培养红绿双色荧光示踪的胶质瘤干/祖细胞与宿主巨噬细胞,置于活细胞工作站实时摄影缩时成像并视频分析目标细胞的社会行为.结果 相关细胞实时动态连续观察发现:(1)细胞间传递信息的连接管道的6种类型.(2)2个活跃细胞相互作用后变成1个细胞,即细胞融合;已融合的细胞与另一个细胞再融合.(3)融合细胞的命运有对称分裂产生子细胞和瞬间凋亡两种.(4)存在一个细胞进入另一细胞内后胞释现象.结论 胶质瘤干/祖细胞与宿主巨噬细胞间连接方式、融合、分裂及胞释过程,可作为胶质瘤干/祖细胞诱导宿主巨噬细胞恶变的细胞行为学依据,对进一步理解肿瘤细胞社会成员间的复杂关系有重要意义.
目的 通過分析免疫細胞社會中的腫瘤細胞與宿主巨噬細胞之間的關繫,探尋腫瘤細胞社會中打破免疫平衡的大事件.方法 共培養紅綠雙色熒光示蹤的膠質瘤榦/祖細胞與宿主巨噬細胞,置于活細胞工作站實時攝影縮時成像併視頻分析目標細胞的社會行為.結果 相關細胞實時動態連續觀察髮現:(1)細胞間傳遞信息的連接管道的6種類型.(2)2箇活躍細胞相互作用後變成1箇細胞,即細胞融閤;已融閤的細胞與另一箇細胞再融閤.(3)融閤細胞的命運有對稱分裂產生子細胞和瞬間凋亡兩種.(4)存在一箇細胞進入另一細胞內後胞釋現象.結論 膠質瘤榦/祖細胞與宿主巨噬細胞間連接方式、融閤、分裂及胞釋過程,可作為膠質瘤榦/祖細胞誘導宿主巨噬細胞噁變的細胞行為學依據,對進一步理解腫瘤細胞社會成員間的複雜關繫有重要意義.
목적 통과분석면역세포사회중적종류세포여숙주거서세포지간적관계,탐심종류세포사회중타파면역평형적대사건.방법 공배양홍록쌍색형광시종적효질류간/조세포여숙주거서세포,치우활세포공작참실시섭영축시성상병시빈분석목표세포적사회행위.결과 상관세포실시동태련속관찰발현:(1)세포간전체신식적련접관도적6충류형.(2)2개활약세포상호작용후변성1개세포,즉세포융합;이융합적세포여령일개세포재융합.(3)융합세포적명운유대칭분렬산생자세포화순간조망량충.(4)존재일개세포진입령일세포내후포석현상.결론 효질류간/조세포여숙주거서세포간련접방식、융합、분렬급포석과정,가작위효질류간/조세포유도숙주거서세포악변적세포행위학의거,대진일보리해종류세포사회성원간적복잡관계유중요의의.
Objective The roles of real-time monitoring of cytokine secretion at the single immunocyte level,using the concept of immune cells sociology have been reported recently.We analyzed the relationships between glioma stem/progenitors cells and host macrophages in the immune cells society,for the purpose of discovering the important events which broke the immune balance of the cancer cells society.Methods The dual fluorescence tracing method was applied to trace the co-cultured glioma stem/progenitor cells and host macrophages,interactions between the two kinds of cells were observed under live cell imaging system,Yellow cells in the co-culture system were mono-cloned,and proliferated in vitro.Then their social activities were observed and recorded under the live cell imaging system.Results Via real-time dynamic observation of the target cells,we noticed that:(1) there were 6 types of conjunction pipes functioned as transfering intercellular information;(2) two activating cells formed hybrid cell after mutual interactions,and the hybrid cell fused with another cell again;(3) the fates of hybrid cell were to generate offspring cells by symmetrical division,or to undergo apoptosis;(4) the phenomenon of cell in cell,which can release out another cell,namely entosis.Conclusion The research revealed the patterns of cell conjunction between glioma stem/progenitor cells and host macrophages,the processes of cell fusion,division and entosis.These were the cell sociology evidences of glioma stem/progenitor cells inducing malignant transformation of host macrophages,which offered basic knowledges exploring the complex relationships among the residents in the tumor cell society.