CAJ | 학술논문

目的：探讨溶血磷脂酸（ LPA ）协同转化生长因子1（ TGFβ1）的促上皮间质转化（ EMT）作用。方法观察TGFβ1及LPA诱导SW480细胞后细胞形态变化，Western blotting方法检测E-Cadherin、Vimentin的表达变化以及细胞增殖活性变化。结果 TGFβ1及LPA均能使SW480细胞形态发生EMT改变；两者联合诱导细胞EMT改变更为明显。空白对照组、LPA组、TGFβ1组、LPA组＋TGFβ1组，E-Cadherin表达依次下降；Vimentin表达未见明显差异。结论 LPA可能协同TGF-β1产生EMT作用，下调LPA的表达或可抑制肿瘤侵袭转移。
목적：탐토용혈린지산（ LPA ）협동전화생장인자1（ TGFβ1）적촉상피간질전화（ EMT）작용。방법관찰TGFβ1급LPA유도SW480세포후세포형태변화，Western blotting방법검측E-Cadherin、Vimentin적표체변화이급세포증식활성변화。결과 TGFβ1급LPA균능사SW480세포형태발생EMT개변；량자연합유도세포EMT개변경위명현。공백대조조、LPA조、TGFβ1조、LPA조＋TGFβ1조，E-Cadherin표체의차하강；Vimentin표체미견명현차이。결론 LPA가능협동TGF-β1산생EMT작용，하조LPA적표체혹가억제종류침습전이。
Objective This study aimed to investigate whether LPA could synergize TGFβ1 to increase epithelial-to-mesenchymal transition(EMT).Methods Western blotting was used to examine the EMT markers:E-cadherin and Vimentin .MTT was used to examine the cell proliferation of 5-Fu and LOHP with or without LPA.Western blotting was used to examine the apoptosis markers Bcl-2,Bax and Caspese-3 after LPA treatment .Results TGFβ1 and LPA induced the morphology change of colorectal cancer cell line SW480 , and combination group LPA/TGFβ1 induced more significant change .Compared with the LPA group,the difference of E-cadherin in blank control group 1%FBS,and TGFβ1 treatment group,the LPA/TGFβ1 co-treatment group were significant ,while the difference of Vimentin was not significant .Conclusion LPA synergizes with TGF-βto play an important role in EMT .Inhibition of LPA may decrease the invasion and migration of colon cancer cells .