CAJ | 학술논문

目的探讨麻疹病毒(MeV)流行株血凝素蛋白(H)抗原表位上氨基酸(aa)变异对病毒抗原性的可能影响.方法利用生物信息学软件预测MeV H蛋白上B细胞线性表位,设计并合成来源于疫苗株和流行株表位以及同一区域非表位上的多肽对.间接ELISA法检测合成多肽的免疫原性,并制备多肽免疫血清.采用交叉ELISA法分析两条多肽间的抗原性差异,计算抗原比.结果合成的多肽均能与MeV免疫血清结合,其中设计在表位区的多肽对CW23/CW22(273～282aa)结合能力最强,而非表位区多肽对CW150/CW151(418～427 aa)结合能力最弱.多肽对中来源不同两条多肽间抗原性差异较大,其中CW23(疫苗株来源)与CW22(流行株来源)间抗原比为16,CW123(疫苗株来源)与CW124(流行株来源)(236 ～ 246aa)间的抗原比为2.877±0.583.非表位多肽对中,CW125与CW126(356 ～ 364aa)间抗原比为1.631±0.481,而CW150与CW151间抗原比为10.367±1.617.结论麻疹流行株上仍存在保守的抗原表位,但预测的抗原表位及非表位区上的部分aa变异导致疫苗株与流行株间抗原性存在差异.
목적 탐토마진병독(MeV)류행주혈응소단백(H)항원표위상안기산(aa)변이대병독항원성적가능영향.방법 이용생물신식학연건예측MeV H단백상B세포선성표위,설계병합성래원우역묘주화류행주표위이급동일구역비표위상적다태대.간접ELISA법검측합성다태적면역원성,병제비다태면역혈청.채용교차ELISA법분석량조다태간적항원성차이,계산항원비.결과 합성적다태균능여MeV면역혈청결합,기중설계재표위구적다태대CW23/CW22(273～282aa)결합능력최강,이비표위구다태대CW150/CW151(418～427 aa)결합능력최약.다태대중래원불동량조다태간항원성차이교대,기중CW23(역묘주래원)여CW22(류행주래원)간항원비위16,CW123(역묘주래원)여CW124(류행주래원)(236 ～ 246aa)간적항원비위2.877±0.583.비표위다태대중,CW125여CW126(356 ～ 364aa)간항원비위1.631±0.481,이CW150여CW151간항원비위10.367±1.617.결론 마진류행주상잉존재보수적항원표위,단예측적항원표위급비표위구상적부분aa변이도치역묘주여류행주간항원성존재차이.
Objective To discuss the antigenic change caused by the mutation of amino acid on the epitopes of the hemagglutinin of measles virus.Methods The B cell linear epitopes in the hemagglutinin were predicted with bioinformatics software.Peptide pairs,which located on the same region but originated from measles vaccine and wild-type virus respectively,were designed and synthesized.After detecting the immunogenicity of peptides with indirect ELISA assay,sera against each peptide was prepared.Antigenic specificity between the two peptides within each peptide pair were tested by using cross ELISA assay,and then antigen ratios were calculated.Results All the synthesized peptides could bind with immune sera against measles virus,of which the peptide pair CW23/CW22 designed on the epitope region (273-282 aa) possessed the highest binding ability,while the peptide pair CW150/CW151 designed on the non-epitope region (418-427 aa) showed the lowest binding ability.The difference in antigenic specificity between the two peptides from different sources was significant.The antigenic ratio was up to 16 between CW23 (vaccine-originated) and CW22 (wild-type originated),and 2.877 ± 0.583 between CW123 (vaccine-originated) and CW124 (wild-type originated) (236-246 aa).On the non-epitope regions,the antigenic ratios was only 1.631 ± 0.481 between peptide pair CW125 and CW126(356-364 aa),but reached to 10.367± 1.617 between CW150 and CW151.Conclusion Although there were several conservative epitopes,specific amino acid mutation on the predicted epitope or non-epitope regions might cause the antigenic change of wild-type measles virus.