CAJ | 학술논문

α输入蛋白存在于胞质溶胶中,是核孔转运复合体的重要组成部分,与核定位信号结合,通过受体介导蛋白物质转入和转出,在此过程中起连接器的作用.该研究以千里光全长 cDNA 文库为基础,对α输入蛋白的序列、结构、性质和功能进行了分析,并在千里光α输入蛋白核苷酸序列的基础上,采用生物信息学软件,分析α输入蛋白的氨基酸序列结构和基因进化树,得到了α输入蛋白的一级、二级、三级等结构和结构域特征,以此为依据,系统分析千里光α输入蛋白的理化性质、结构和功能.结果表明：千里光α输入蛋白基因编码529个氨基酸,与烟草(GenBank登录号：ABM05487．1)的同源性最高,为84％；蛋白质分子量58．46 kDa,理论等电点5．08；二级结构由α螺旋、无规则卷曲和延伸主链构成；高级结构域由 IBB 和 ARM结构构成；三级结构是4个功能结构域构成的空间立体结构.此外,还发现千里光α输入蛋白具有调控激素反应、传输信号、细胞生长、信息转录和转录调控功能的概率较高,推测可能与细胞非凋亡性死亡、抗病性防御反应、激素受体反应以及基因转录调控表达密切相关.该研究结果可为其他物种α输入蛋白结构与功能关系的分析提供参考.
α수입단백존재우포질용효중,시핵공전운복합체적중요조성부분,여핵정위신호결합,통과수체개도단백물질전입화전출,재차과정중기련접기적작용.해연구이천리광전장 cDNA 문고위기출,대α수입단백적서렬、결구、성질화공능진행료분석,병재천리광α수입단백핵감산서렬적기출상,채용생물신식학연건,분석α수입단백적안기산서렬결구화기인진화수,득도료α수입단백적일급、이급、삼급등결구화결구역특정,이차위의거,계통분석천리광α수입단백적이화성질、결구화공능.결과표명：천리광α수입단백기인편마529개안기산,여연초(GenBank등록호：ABM05487．1)적동원성최고,위84％；단백질분자량58．46 kDa,이론등전점5．08；이급결구유α라선、무규칙권곡화연신주련구성；고급결구역유 IBB 화 ARM결구구성；삼급결구시4개공능결구역구성적공간입체결구.차외,환발현천리광α수입단백구유조공격소반응、전수신호、세포생장、신식전록화전록조공공능적개솔교고,추측가능여세포비조망성사망、항병성방어반응、격소수체반응이급기인전록조공표체밀절상관.해연구결과가위기타물충α수입단백결구여공능관계적분석제공삼고.
Alpha Importin is located in cytosolic space and is an important part of nuclear pore complex (NPC).After integrating with nuclear localization signal (NLS),it plays an essential role in mediating proteins’nucleocytoplasmic transporting as a connector.In the current study,to systematically explore the properties of physicochemical,se-quence structure,function and the relationships between structural domain and its function ofα-Importin,its nucleo-tide sequence was selected from a former constructed full-length cDNA library in Senecio scandens for probing its se-quence features and composition,and gene’s function by applying a series of bioinformatics software.The structure properties of primary,secondary,domain and tertiary of the amino acid sequence were analyzed,too.And the phylo-genetic relationships amongα-Importin of S.scandens with other species were also constructed.These exploration found that this gene encoded a length of 529 amino acid polypeptide with the calculated molecular weight of 58.46 kDa,and its theoretical isoelectric point was 5.08.It shared 84.0% identity with tobaccoα-Importin gene (GenBank ID:ABM05487.1)by amino acid sequence alignment.The structure analysis showed its secondary structure was composed ofαhelix protein,a random coil and an extended main-chain.The function domain probing found the target gene was composed domains of IBB and ARM.While its three-dimensional structure was mainly consisted of four structural domains.In conclusion,this study found that the function of thisα-Importin gene was regulation of hor-mone secretion,a signal transducer,growth factor,transcription and transcription regulation with higher rank of probability ratio.The current study also founded that thisα-Importin gene could play important roles in many biology processes such as non-apoptotic programmed cell death,defence response of higher plants against pathogens,hor-mone receptor effect and gene transcription reactions and expression.According to the exploring of the properties of gene sequences,structure and function ofα-Importin in S.scandens,this paper would provide a reference for similar analysis of the relationship between the structure and function ofα-Importin genes in other species.