CAJ | 학술논문

为探索外源激素对灰毡毛忍冬‘渝蕾1号’愈伤组织生长及其代谢产物绿原酸含量的影响,该研究通过在培养基中添加不同种类和浓度的外源激素,以诱导其愈伤组织的产生及增殖生长,并采用称量法和高效液相色谱法分别检测愈伤组织生物量和绿原酸含量.结果表明：不同外源激素及其组合对愈伤组织生物量和绿原酸含量的积累产生不同的影响；培养基中添加单一外源激素时,对愈伤组织生物量影响最大的为 TDZ激素；和对照相比,不同浓度的TDZ均使生物量显著提高,其中0．5 mg?L-1的TDZ使生物量达最大,为0．62 g；对绿原酸积累影响的结果不太一致,除TDZ激素影响最小以外,0．5 mg?L-1的 NAA使愈伤组织中的绿原酸含量积累最多,为11．18 mg?g-1；外源激素组合中,含有 TDZ激素的组合生物量偏高,但绿原酸含量偏低,这与单一外源激素的结果一致.MS＋0．5 mg?L-1 TDZ＋0．5 mg?L-12,4-D培养基使愈伤组织中绿原酸含量积累较多,为15．53 mg?g-1,且生物量达最高,为0．65 g,因此该激素组合为该研究最适培养基组合.通过筛选适宜的外源激素及其组合能促进灰毡毛忍冬‘渝蕾1号’愈伤组织生物量的提高和次生代谢产物绿原酸的积累.研究结果对从灰毡毛忍冬‘渝蕾1号’愈伤组织中获得大量的绿原酸提供了重要依据.
위탐색외원격소대회전모인동‘투뢰1호’유상조직생장급기대사산물록원산함량적영향,해연구통과재배양기중첨가불동충류화농도적외원격소,이유도기유상조직적산생급증식생장,병채용칭량법화고효액상색보법분별검측유상조직생물량화록원산함량.결과표명：불동외원격소급기조합대유상조직생물량화록원산함량적적루산생불동적영향；배양기중첨가단일외원격소시,대유상조직생물량영향최대적위 TDZ격소；화대조상비,불동농도적TDZ균사생물량현저제고,기중0．5 mg?L-1적TDZ사생물량체최대,위0．62 g；대록원산적루영향적결과불태일치,제TDZ격소영향최소이외,0．5 mg?L-1적 NAA사유상조직중적록원산함량적루최다,위11．18 mg?g-1；외원격소조합중,함유 TDZ격소적조합생물량편고,단록원산함량편저,저여단일외원격소적결과일치.MS＋0．5 mg?L-1 TDZ＋0．5 mg?L-12,4-D배양기사유상조직중록원산함량적루교다,위15．53 mg?g-1,차생물량체최고,위0．65 g,인차해격소조합위해연구최괄배양기조합.통과사선괄의적외원격소급기조합능촉진회전모인동‘투뢰1호’유상조직생물량적제고화차생대사산물록원산적적루.연구결과대종회전모인동‘투뢰1호’유상조직중획득대량적록원산제공료중요의거.
Influence of exogenous hormones on the growth of biomass and the generation of chlorogenic acid in callus of Lonicera macranthoides‘Yulei 1’was investigated in this study.Different kinds and concentrations of exogenous hormones were added in culture media to induce the proliferation anddifferentiation of callus.The biomass and a-mount of chlorogenic acid in callus were analyzed by weight and HPLC.The result showed that the biomass of callus and amount of chlorogenic acid were affected by variation of hormones.TDZ in all hormones had the strongest influ-ence on the biomass of callus when single exogenous hormone was added.It reached the maximum of 0.62 g in 0.5 mg?L-1 TDZ.On the other hand,TDZ had the least influence on the generation of chlorogenic acid,whereas 0.5 mg?L-1 of NAA had the highest amount (1 1 .1 8 mg?g-1 ).In the groups of combination of hormones,the ones with TDZ generated abundant biomass but low chlorgenic acid.The group of MS+TDZ 0.5 mg?L-1+ 2,4-D 0.5 mg?L-1 which had the highest biomass (0.65 g)and relatively high chlorogenic acid (15.53 mg?g-1),was the most appro-priate culture media.Therefore,this study provides an important basis on obtaining enormous chlorogenic acid from cal-lus of L.macranthoides ‘Yulei 1’through screening the different exogenous hormones and their combinations.