CAJ | 학술논문

目的探讨血卟啉单甲醚光动力治疗(HMME-PDT)处理后人胶质瘤U251细胞长链非编码RNA(lncRNA)表达谱的变化.方法使用HMME-PDT处理人胶质瘤U251细胞,提取RNA,体外反转录制备并标记为双链cDNA,在标准条件下与含有33 045条lncRNA及30215条mRNA的芯片杂交,计算机扫描荧光信号图像,分析lncRNA和mRNA表达谱的变化.结果在HMME-PDT处理的人U251细胞中,lncRNA表达上调l 121条,表达下调1 025条;mRNA表达上调1 111条,表达下调935条(变化＞2倍且P＜ 0.05).结论 HMME-PDT处理后人胶质瘤细胞lncRNA/mRNA发生明显变化,lncRNA与光动力处理后抗原呈递元件(APM)表达变化密切相关.
목적 탐토혈계람단갑미광동력치료(HMME-PDT)처리후인효질류U251세포장련비편마RNA(lncRNA)표체보적변화.방법 사용HMME-PDT처리인효질류U251세포,제취RNA,체외반전록제비병표기위쌍련cDNA,재표준조건하여함유33 045조lncRNA급30215조mRNA적심편잡교,계산궤소묘형광신호도상,분석lncRNA화mRNA표체보적변화.결과 재HMME-PDT처리적인U251세포중,lncRNA표체상조l 121조,표체하조1 025조;mRNA표체상조1 111조,표체하조935조(변화＞2배차P＜ 0.05).결론 HMME-PDT처리후인효질류세포lncRNA/mRNA발생명현변화,lncRNA여광동력처리후항원정체원건(APM)표체변화밀절상관.
Objective To study hematoporphyrin monomethyl ether photodynamic therapy (HMME-PDT) treatment posterity glioma U251 cell lines long chain noncoding RNA (lncRNA) expression changes,and the results are analyzed.Methods Using HMME-PDT treatment of glioma U251 cell lines,preparation and extraction of RNA reverse transcription in vitro markers for double chain cDNA,under the condition of the standard and contains 33 045 long non-coding RNAs (lncRNA) and 33 045 mRNA chip hybridization,computer scanning fluorescent signal image,analyzing the change of the lncRNA/mRNA expression.Results In HMME-before and after treatment were treated with PDT U251 cells,raised 1 121 lncRNA,express by 1 025;Raised 1 111 mRNA,cut in 935 (＞2 times and P＜ 0.05).Conclusion HMME-PDT treatment posterity glioma cells IncRNA has obvious changes,antigen processing machinery (APM) lncRNA antigen-presenting after PDT treatment related protein expression changes have close relationship.