CAJ | 학술논문

为获得豇豆(Vigna unguiculata)遗传转化体系，以‘成豇七号’带1片子叶的子叶节作为外植体，对其高效再生体系和农杆菌介导抗病基因Pti4的遗传转化进行了研究。结果表明，豇豆无菌苗、不定芽诱导和不定芽伸长培养的最适培养基分别为MSB5+6-BA 3.0 mg L–1、MSB5+6-BA 1.0 mg L–1+ KT 0.06 mg L–1和MSB5+6-BA 0.5 mg L–1+ IBA 0.2 mg L–1。不定芽在MS培养基上能迅速诱导生根，获得完整植株。以豇豆子叶节为受体，通过农杆菌介导成功将Pti4整合到‘成豇七号’抗性芽基因组中。因此，豇豆高效再生体系的建立为遗传育种研究奠定了基础。
위획득강두(Vigna unguiculata)유전전화체계，이‘성강칠호’대1편자협적자협절작위외식체，대기고효재생체계화농간균개도항병기인Pti4적유전전화진행료연구。결과표명，강두무균묘、불정아유도화불정아신장배양적최괄배양기분별위MSB5+6-BA 3.0 mg L–1、MSB5+6-BA 1.0 mg L–1+ KT 0.06 mg L–1화MSB5+6-BA 0.5 mg L–1+ IBA 0.2 mg L–1。불정아재MS배양기상능신속유도생근，획득완정식주。이강두자협절위수체，통과농간균개도성공장Pti4정합도‘성강칠호’항성아기인조중。인차，강두고효재생체계적건립위유전육충연구전정료기출。
In order to obtain genetic transformation system of cowpea (Vigna unguiculata), its cotyledon nodes with one cotyledon of ‘Chengjiang 7’ was used as explants, the regeneration system andPti4 genetic transformation system byAgrobacterium-medium were studied. The results showed that the optimum mediums for aseptic seedling, adventitious bud introduction and adventitious bud elongation were MSB5 + 6-BA 3.0 mg L–1, MSB5 + 6-BA 1.0 mg L–1 + KT 0.06 mg L–1 and MSB5 + 6-BA 0.5 mg L–1 + IBA 0.2 mg L–1, respectively. Adventitious buds could rapidly rooting on MS medium to obtain full plantlets. The cotyledon node was used as receptor, thepit4 gene mediated byAgrobacterium was successfully transformed into the genome of resistant buds of ‘Chengjiang 7’. Therefore, the establishment of high efifcient regeneration system for cowpea laid basis for studying on genetic transformation.