CAJ | 학술논문

目的：研究侧裂区低级别脑胶质瘤中基质金属蛋白酶( MMP)家族水平的检测及其与预后情况的相关性。方法将2007年1月至2010年12月四川大学华西医院收治的130例侧裂区Ⅰ、Ⅱ级胶质瘤患者作为观察组,选择同期收治的30例颅脑损伤患者作为对照组,检测组织中MMP-2、MMP-9的mRNA和蛋白水平,并随访观察组患者的预后情况。结果观察组患者肿瘤组织中MMP-2、MMP-9的mRNA水平分别为233±35、253±32,均高于对照组的100±15、100±19,差异有统计学意义( P <0．05)；观察组患者肿瘤组织中 MMP-2、MMP-9的蛋白阳性率分别为63．8%(83/130)、62．3%(81/130),均高于对照组的13．3%(4/30)、16．7%(5/30),差异有统计学意义(P <0．05)。 MMP-2、MMP-9阳性患者的无瘤生存时间短于阴性患者,1年复发率、3年复发率高于阴性患者(P<0．05)；MMP-2、MMP-9是胶质瘤患者1年复发(OR=2．275,2．311)、3年复发(OR=2．291,2．541)的危险因素。 MMP-2、MMP-9阳性患者的无瘤生存时间短于阴性患者(P<0．05),1年复发率、3年复发率高于阴性患者(P<0．05)；MMP-2、MMP-9是胶质瘤患者1年复发、3年复发的危险因素。结论 MMP-2、MMP-9的表达与侧裂区脑胶质瘤患者的预后密切相关,MMP-2、MMP-9阳性患者的远期复发率较高。
목적：연구측렬구저급별뇌효질류중기질금속단백매( MMP)가족수평적검측급기여예후정황적상관성。방법장2007년1월지2010년12월사천대학화서의원수치적130례측렬구Ⅰ、Ⅱ급효질류환자작위관찰조,선택동기수치적30례로뇌손상환자작위대조조,검측조직중MMP-2、MMP-9적mRNA화단백수평,병수방관찰조환자적예후정황。결과관찰조환자종류조직중MMP-2、MMP-9적mRNA수평분별위233±35、253±32,균고우대조조적100±15、100±19,차이유통계학의의( P <0．05)；관찰조환자종류조직중 MMP-2、MMP-9적단백양성솔분별위63．8%(83/130)、62．3%(81/130),균고우대조조적13．3%(4/30)、16．7%(5/30),차이유통계학의의(P <0．05)。 MMP-2、MMP-9양성환자적무류생존시간단우음성환자,1년복발솔、3년복발솔고우음성환자(P<0．05)；MMP-2、MMP-9시효질류환자1년복발(OR=2．275,2．311)、3년복발(OR=2．291,2．541)적위험인소。 MMP-2、MMP-9양성환자적무류생존시간단우음성환자(P<0．05),1년복발솔、3년복발솔고우음성환자(P<0．05)；MMP-2、MMP-9시효질류환자1년복발、3년복발적위험인소。결론 MMP-2、MMP-9적표체여측렬구뇌효질류환자적예후밀절상관,MMP-2、MMP-9양성환자적원기복발솔교고。
Objective To study the levels of matrix metalloproteinase( MMP) family in lateral fissure area low grade glioma and its correlation with prognosis. Methods Total of 130 patients with lateral fissure area gradeⅠandⅡglioma in West China Hospital of Sichuan University from Jan. 2007 to Dec. 2010 were enrolled in observation group and another 30 brain injury patients in the same period were enrolled in control group. Then mRNA and protein levels of MMP-2,MMP-9 were detected,and the prognosis were followed up. Results mRNA levels of MMP-2,MMP-9 in tumor tissue of the observation group were 233 ± 35 and 253 ± 32,higher than the control group(100 ±15 and 100 ±19),the differences were statistically significant(P<0. 05). The positive rate of MMP-2,MMP-9 protein in tumor tissue of the observation group were 63. 8%(83/130) and 62.3%(81/130),which were higher than the control group[13.3%(4/30) and 16.7%(5/30)],the differences were statistically significant(P <0. 05). Tumor free survival time of MMP-2,MMP-9 positive patients was shorter than negative patients, and the 1-year and 3-year recurrence rate were higher than the negative patients(P <0. 05). MMP-2 and MMP-9 were risk factors of 1-year recurrence(OR =2. 275,2. 311)and 3-year recurrence(OR=2. 291,2. 541). Conclusion MMP-2 and MMP-9 expression are closely correlated with prognosis of lateral fissure area glioma patients;long-term recurrence rate of MMP-2, MMP-9 positive patients is higher.