CAJ | 학술논문

目的：探究不同乙型肝炎病毒(HBV) DNA载量患者磷脂酰肌醇-4-磷酸酶(PI4Kα)的表达差异及其意义。方法收集2013年1月至2015年1月在天门市第一人民医院接受治疗的150例慢性乙型肝炎患者及50例同时期体检健康人群的血清,利用荧光定量聚合酶链反应( FQ-PCR)检测所有研究对象血清的HBV DNA载量,并根据患者血清HBV DNA载量分为高病毒载量组( HBV DNA>1×105拷贝/L)、中病毒载量组( HBV DNA 1×102~1×105拷贝/L)及低病毒载量组( HBV DNA<1×102拷贝/L)3组,各50例。另外选择50例健康体检者的血清作为对照组。比较4组血清HBV DNA载量,采用酶联免疫吸附测定(ELISA)双抗体夹心法对4组外周血清的PI4Kα水平进行检测,分析HBV DNA载量与PI4Kα表达的相关性。结果高、中、低病毒载量组及对照组HBV DNA载量和血清PI4Kα水平分别为(5．6±1．3)拷贝/L、(3．5±0．6)拷贝/L、(1．7±0．5)拷贝/L、(1．1±0．3)拷贝/L和(4．3±0．8)μg/L、(3．4±0．7)μg/L、(2．8±0．7)μg/L、(2．2±0．6)μg/L,各组比较差异有统计学意义(P<0．01),其中高、中及低病毒载量组HBV DNA载量和血清PI4Kα水平显著高于对照组(P<0．05),高、中病毒载量组显著高于低病毒载量组(P<0．05),高病毒载量组显著高于中病毒载量组(P<0．05)。随着血清HBV DNA载量的增加,PI4Kα水平也显著升高,两者呈正相关(rs =0．732,P<0．05)。结论慢性乙型肝炎患者血清PI4Kα的水平随着HBV DNA载量的增加而增加,两者密切相关,提示PI4Kα对HBV DNA的复制有重要作用。
목적：탐구불동을형간염병독(HBV) DNA재량환자린지선기순-4-린산매(PI4Kα)적표체차이급기의의。방법수집2013년1월지2015년1월재천문시제일인민의원접수치료적150례만성을형간염환자급50례동시기체검건강인군적혈청,이용형광정량취합매련반응( FQ-PCR)검측소유연구대상혈청적HBV DNA재량,병근거환자혈청HBV DNA재량분위고병독재량조( HBV DNA>1×105고패/L)、중병독재량조( HBV DNA 1×102~1×105고패/L)급저병독재량조( HBV DNA<1×102고패/L)3조,각50례。령외선택50례건강체검자적혈청작위대조조。비교4조혈청HBV DNA재량,채용매련면역흡부측정(ELISA)쌍항체협심법대4조외주혈청적PI4Kα수평진행검측,분석HBV DNA재량여PI4Kα표체적상관성。결과고、중、저병독재량조급대조조HBV DNA재량화혈청PI4Kα수평분별위(5．6±1．3)고패/L、(3．5±0．6)고패/L、(1．7±0．5)고패/L、(1．1±0．3)고패/L화(4．3±0．8)μg/L、(3．4±0．7)μg/L、(2．8±0．7)μg/L、(2．2±0．6)μg/L,각조비교차이유통계학의의(P<0．01),기중고、중급저병독재량조HBV DNA재량화혈청PI4Kα수평현저고우대조조(P<0．05),고、중병독재량조현저고우저병독재량조(P<0．05),고병독재량조현저고우중병독재량조(P<0．05)。수착혈청HBV DNA재량적증가,PI4Kα수평야현저승고,량자정정상관(rs =0．732,P<0．05)。결론만성을형간염환자혈청PI4Kα적수평수착HBV DNA재량적증가이증가,량자밀절상관,제시PI4Kα대HBV DNA적복제유중요작용。
Objective To explore the difference of the expression of phosphatidylinositol 4-kinase Ⅲalpha (P14Kα)in serum between patients with different hepatitis B virus(HBV) DNA loads and its signifi-cance. Methods Serum samples were collected from 150 patients with chronic hepatitis B( CHB) and 50 healthy people in Tianmen City First Hospital during Jan. 2013 and Jan. 2015,whose HBV DNA loads were determined by fluorescent quantitative polymerase chain reaction ( FQ-PCR ) . According to the serum HBV DNA loads,the 150 patients were divided into three groups:high viral load group(HBV DNA load>1 ×105 copies/L),medium viral load group(1 ×105 copies/L≤HBV DNA≤1 ×102 copies/L),and low viral load group(HBV DNA load<1 ×102 copies/L),while the serum samples of 50 healthy people were selected as control group. The HBV DNA loads in serum between the four groups were compared. Enzyme-linked immu-nosorbent assay was used to determine the concentration of PI4Kα in peripheral serum,Spearman inspection was used to analyze the correlation between HBV DNA loads and the expression level of PI4Kα. Results HBV DNA load and serum PI4Kα level of high,medium and low viral load group and control group were (5. 6 ± 1. 3) copies/L,(3. 5 ± 0. 6) copies/L,(1. 7 ± 0. 5) copies/L,(1. 1 ± 0. 3) copies/L and (4. 3 ± 0. 8) μg/L,(3. 4 ± 0. 7) μg/L,(2. 8 ± 0. 7) μg/L,(2. 2 ± 0. 6) μg/L,there were significant differences between the four groups(P<0. 05). Among them,HBV DNA load and serum PI4Kα level of the high and low viral load were significantly higher than that of the control group (P<0. 05),and the high and medium viral load group was significantly higher than that of the low viral load group (P<0. 05),high viral load group was significantly higher than that of the low viral load group (P <0. 05). The HBV DNA load and PI4Kαconcentration presented a positive correlation with each other, i. e. serum PI4Kα level would be elevated with the increase in HBV DNA load(rs =0. 732,P<0. 05). Conclusion Serum PI4Kα level is elevated with the increase in HBV DNA load,the concentration of serum PI4Kα is closely correlated with the HBV DNA load in patients with CHB,which suggests that PI4Kα may play a crucial role in the HBV DNA replication.