CAJ | 학술논문

为探讨香蕉(Musa acuminata)响应冷胁迫的分子机制，从香蕉果实冷害的数字基因表达谱中筛选并分离了1个WRKY转录因子，命名为MaWRKY11。MaWRKY11具有2个WRKY保守结构域，属于I类WRKY成员，定位于细胞核，是核蛋白。MaWRKY11具有转录激活活性，且激活区在N端。实时荧光定量PCR分析表明MaWRKY11受冷胁迫诱导，外源茉莉酸甲酯(MeJA)处理减轻香蕉果实冷害的同时也上调了其表达。另外，酵母双杂交筛选表明，MaWRKY11可与脱水诱导的早期应答蛋白MaERD相互作用。这些表明MaWRKY11可能通过与逆境相关蛋白如MaERD互作来响应香蕉果实的冷胁迫。
위탐토향초(Musa acuminata)향응랭협박적분자궤제，종향초과실냉해적수자기인표체보중사선병분리료1개WRKY전록인자，명명위MaWRKY11。MaWRKY11구유2개WRKY보수결구역，속우I류WRKY성원，정위우세포핵，시핵단백。MaWRKY11구유전록격활활성，차격활구재N단。실시형광정량PCR분석표명MaWRKY11수랭협박유도，외원말리산갑지(MeJA)처리감경향초과실냉해적동시야상조료기표체。령외，효모쌍잡교사선표명，MaWRKY11가여탈수유도적조기응답단백MaERD상호작용。저사표명MaWRKY11가능통과여역경상관단백여MaERD호작래향응향초과실적랭협박。
In order to understand the molecular mechanism, oneWRKY transcription factor, named asMaWRKY11, was isolated and identiifed from Digital Gene Expression Data (DGE) of banana (Musa acuminata) fruits. The results showed that MaWRKY11 contained two WRKY conserved domains, belonging to Group I of WRKY family, and located in nucleus as a nuclear protein. The MaWRKY11 possessed transcriptional activity in yeast cells and the transcriptional domain located at its N-terminus. Moreover, real-time quantitative PCR displayed thatMaWRKY11 was induced by cold stress, and chilling injury of banana fruits reduced and the expression of MaWRKY11 was up-regulated treated by MeJA. In addition, C-terminus of MaWRKY11 was used as a bait to screen its potential interacting proteins by using yeast two-hybrid system, and it was found that MaWRKY11 could interact with early responsive to dehydration protein, MaERD, which was also induced by cold. So, it was suggested that MaWRKY11 might respond to chilling stress through interacting with stress-related proteins, such as MaERD.