CAJ | 학술논문

采用 RP －HPLC 法建立了忍冬藤中绿原酸、马钱苷、当药苷三种成分的含量测定方法。色谱条件为：Aglient TC －C18（4．6 mm ×250 mm，5μm）色谱柱；流动相：A 为乙腈，B 为0．4％磷酸溶液，进行梯度洗脱，流速为1．0 mL／min；检测波长为240 nm，柱温25℃。在该色谱条件下，所测定三种成分均达到基线分离，且各成分在线性范围内线性关系良好，精密度、重现性、稳定性均良好，回收率变化范围为98．65％～99．07％。该方法准确、简便、可行，可以用于忍冬藤的质量控制。
채용 RP －HPLC 법건립료인동등중록원산、마전감、당약감삼충성분적함량측정방법。색보조건위：Aglient TC －C18（4．6 mm ×250 mm，5μm）색보주；류동상：A 위을정，B 위0．4％린산용액，진행제도세탈，류속위1．0 mL／min；검측파장위240 nm，주온25℃。재해색보조건하，소측정삼충성분균체도기선분리，차각성분재선성범위내선성관계량호，정밀도、중현성、은정성균량호，회수솔변화범위위98．65％～99．07％。해방법준학、간편、가행，가이용우인동등적질량공제。
A new method for simultaneously determining the contents of chlorogenic acid,loganin and chiratin in Lonicerae japonicae caulis was set up by RP -HPLC.The chromatographic conditions were as Aglient TC -C18 column (4.6 mm ×250 mm,5μm),acetonitrile (A)and 0.4% (V /V)phosphoric acid solution (B)composed as the mobile phase for gradient elution,1.0 mL/min of flow rate,240 nm of detec-tion wavelength and 25℃ of column temperature.The results showed that all the three components were sepa-rated well and their calibration curves showed good linear regression within test ranges;and the precision,re-producibility and stability of this method were better and the recovery rates ranged from 98.65% to 99.07%. In conclusion,this method was accurate,reliable and convenient,so it could be used for the quality evalua-tion of Lonicerae japonicae caulis.