CAJ | 학술논문

目的：探讨新的生物诊断标记物提高对非霍奇金淋巴瘤（NHL）的诊断准确性及其对于疾病的诊断、疗效评估及病情监控的意义，探讨IgH基因序列重组作为新型生物诊断标记物的可行性。方法首先进行 NHL的二代测序（NGS），然后用 PCR 技术检测 NHL患者血浆特异性DNA片段的重组情况。结果通过对12例NHL患者的肿瘤组织IgH基因组区域进行平行DNA捕获和测序（IgCap），准确定位和检测出IgH位点重组序列。另外，我们还获得了一名患者的血浆循环DNA重组片段。结论 NHL患者的血浆中可检测到IgH基因重组，IgCap可能成为一种新的针对NHL患者的临床诊断、疗效评估及病情监控的生物诊断标记物。
목적：탐토신적생물진단표기물제고대비곽기금림파류（NHL）적진단준학성급기대우질병적진단、료효평고급병정감공적의의，탐토IgH기인서렬중조작위신형생물진단표기물적가행성。방법수선진행 NHL적이대측서（NGS），연후용 PCR 기술검측 NHL환자혈장특이성DNA편단적중조정황。결과통과대12례NHL환자적종류조직IgH기인조구역진행평행DNA포획화측서（IgCap），준학정위화검측출IgH위점중조서렬。령외，아문환획득료일명환자적혈장순배DNA중조편단。결론 NHL환자적혈장중가검측도IgH기인중조，IgCap가능성위일충신적침대NHL환자적림상진단、료효평고급병정감공적생물진단표기물。
ObjectiveNovel biomarkers for improving accuracy could be beneficial for disease monitoring and surveillance in patients with non-Hodgkin’s lymphomas (NHL). So we explored the viability of identifying the rearranged IgH genes sequence.MethodsNext-generation sequencing (NGS) was used, and then detected specific rearranged DNA fragments from plasma of NHL patients by PCR. ResultsBy parallel DNA capturing and sequencing of IgH genomic regions (IgCap), the sequence of rearranged IgH loci could be detected and precisely determined in tumor tissues of 12 NHL patients. The circulating rearranged DNA fragments had been identified in the plasma of one patient.ConclusionIgCap may be the favorable diagnostic method for patients with NHL in clinical.