CAJ | 학술논문

目的：建立基于整体柱的表面增强拉曼光谱（surface enhanced Raman spectroscopy ，SERS）法快速检测非法染色的青黛。方法采用混合溶剂乙醇-水（1∶1，V／V）对染色青黛中的染料进行提取，利用整体柱中网状孔隙有助于银纳米颗粒在空间上耦合的特性，将样品溶液和银胶溶液混匀后滴加于整体柱上进行SERS光谱采集。结果此法可得到青黛中染色掺伪量低至500μg／kg的孔雀石绿SERS信号。结论该方法具有快速、简便、专属性强等优点，可运用于中药材青黛非法染色的快速检测。
목적：건립기우정체주적표면증강랍만광보（surface enhanced Raman spectroscopy ，SERS）법쾌속검측비법염색적청대。방법채용혼합용제을순-수（1∶1，V／V）대염색청대중적염료진행제취，이용정체주중망상공극유조우은납미과립재공간상우합적특성，장양품용액화은효용액혼균후적가우정체주상진행SERS광보채집。결과차법가득도청대중염색참위량저지500μg／kg적공작석록SERS신호。결론해방법구유쾌속、간편、전속성강등우점，가운용우중약재청대비법염색적쾌속검측。
Objective To develop a rapid SERS detection method based on monolithic column for detection of dye adul-terated natural indigo .Methods The dyes in natural indigo were extracted and mixed with silver colloid .The spectra were re-corded after applying the mixture solution to the monolithic column since the intertwined pores in monolithic column could con-tribute for the distribution of silver nanoparticles .Results SERS signals of malachite green dyed natural indigo at quantity as low as 500 μg/kg could be obtained .Conclusion This simple ,fast and specific SERS detection method based on monolithic col-umn could be used for rapid detection of stained natural indigo .