中国烧伤创疡杂志
中國燒傷創瘍雜誌
중국소상창양잡지
The Chinese Journal of Burns Wounds And Surface Ulcers
2015年
5期
326-334
,共9页
李浩%彭程%仲昭%贺全勇
李浩%彭程%仲昭%賀全勇
리호%팽정%중소%하전용
湿润烧伤膏%深Ⅱ度创面%PDGF%EGF
濕潤燒傷膏%深Ⅱ度創麵%PDGF%EGF
습윤소상고%심Ⅱ도창면%PDGF%EGF
Moist Exposed Scald Ointment (MEBO)%Deep second degree wound%Platelet-derived growth factor (PDGF)%Epidermal growth factor (EGF)
目的：研究湿润烧伤膏对SD大鼠深Ⅱ度烫伤创面的影响及作用机制。方法将60只SD大鼠制成深Ⅱ度烫伤模型，并按照随机数表法分为湿润烧伤膏组（30只），创面外用湿润烧伤膏换药治疗；磺胺嘧啶银组（30只），创面外用磺胺嘧啶银换药治疗。于烫伤后第3天和第7天通过免疫组化法观察CD31、 Ki67在创面上的表达，作为创面肉芽组织增生情况的评判指标；测定大鼠烫伤创面愈合率，作为判断创面愈合情况的指标；采用荧光定量PCR法测定两组大鼠血小板源性生长因子及表皮生长因子的表达量。结果以愈合率达到95％为创面愈合，实验组创面平均愈合时间为17 d，对照组创面平均愈合时间为20 d，两组创面愈合率进行配对t检验， P＜0?05，差异具有统计学意义。免疫组化法观察结果显示，实验组 CD31及 Ki68的表达均强于对照组。Real?Time PCR结果显示，实验组PDGF （血小板源性生长因子）在伤后1～7 d的表达量均强于对照组； EGF （表皮生长因子）在伤后3～10 d的表达量也均强于对照组。结论湿润烧伤膏能通过增加PDGF、 EGF的表达促进创面血管新生及细胞增殖，加速上皮化进程，达到防止创面加深、促进创面愈合的作用。
目的：研究濕潤燒傷膏對SD大鼠深Ⅱ度燙傷創麵的影響及作用機製。方法將60隻SD大鼠製成深Ⅱ度燙傷模型，併按照隨機數錶法分為濕潤燒傷膏組（30隻），創麵外用濕潤燒傷膏換藥治療；磺胺嘧啶銀組（30隻），創麵外用磺胺嘧啶銀換藥治療。于燙傷後第3天和第7天通過免疫組化法觀察CD31、 Ki67在創麵上的錶達，作為創麵肉芽組織增生情況的評判指標；測定大鼠燙傷創麵愈閤率，作為判斷創麵愈閤情況的指標；採用熒光定量PCR法測定兩組大鼠血小闆源性生長因子及錶皮生長因子的錶達量。結果以愈閤率達到95％為創麵愈閤，實驗組創麵平均愈閤時間為17 d，對照組創麵平均愈閤時間為20 d，兩組創麵愈閤率進行配對t檢驗， P＜0?05，差異具有統計學意義。免疫組化法觀察結果顯示，實驗組 CD31及 Ki68的錶達均彊于對照組。Real?Time PCR結果顯示，實驗組PDGF （血小闆源性生長因子）在傷後1～7 d的錶達量均彊于對照組； EGF （錶皮生長因子）在傷後3～10 d的錶達量也均彊于對照組。結論濕潤燒傷膏能通過增加PDGF、 EGF的錶達促進創麵血管新生及細胞增殖，加速上皮化進程，達到防止創麵加深、促進創麵愈閤的作用。
목적：연구습윤소상고대SD대서심Ⅱ도탕상창면적영향급작용궤제。방법장60지SD대서제성심Ⅱ도탕상모형，병안조수궤수표법분위습윤소상고조（30지），창면외용습윤소상고환약치료；광알밀정은조（30지），창면외용광알밀정은환약치료。우탕상후제3천화제7천통과면역조화법관찰CD31、 Ki67재창면상적표체，작위창면육아조직증생정황적평판지표；측정대서탕상창면유합솔，작위판단창면유합정황적지표；채용형광정량PCR법측정량조대서혈소판원성생장인자급표피생장인자적표체량。결과이유합솔체도95％위창면유합，실험조창면평균유합시간위17 d，대조조창면평균유합시간위20 d，량조창면유합솔진행배대t검험， P＜0?05，차이구유통계학의의。면역조화법관찰결과현시，실험조 CD31급 Ki68적표체균강우대조조。Real?Time PCR결과현시，실험조PDGF （혈소판원성생장인자）재상후1～7 d적표체량균강우대조조； EGF （표피생장인자）재상후3～10 d적표체량야균강우대조조。결론습윤소상고능통과증가PDGF、 EGF적표체촉진창면혈관신생급세포증식，가속상피화진정，체도방지창면가심、촉진창면유합적작용。
Objective To study the mechanism and effect of MEBO in treatment of SD rats with deep second de?gree scald wounds. Methods Used 60 SD rats to establish deep second degree scald models and randomly divided them in?to two groups to observe the scald healing rate:30 rats in the experimental group were treated with MEBO while sulfadiazine silver was used in the control group of 30 rats. Used immunohistochemical method to observe the expression of CD31 and Ki67 in the scald wounds on the 3rd day and 7th day of the treatment, the index of which is the evaluation of the wound granulation tissue growth condition; wound healing rate of the SD rats scald was measured as the index of evaluating the wound healing condition; FQ?PCR method was used to measure the expression quantity of platelet?derived growth factor ( PDGF) and epidermal growth factor ( EGF) . Results Taking the healing rate of 95% or more as wound healed, and the average healing time was 17 days in experimental group, and 20 days in control group. According to the t pair test of the wound healing rate in both groups, P<0?05, the difference had statistical significance. Immunohistochemical examination result showed that the expression of CD31 and Ki68 of the experimental group was stronger than the control group. Real?Time PCR result showed that PDGF expression quantity of the experimental group was stronger than the control group within 1~7 days after injuries;EGF expression quantity of the experimental group was stronger than the control group within 3~10 days after injuries. Conclusion By increasing the expression of PDGF and EGF, MEBO can promote angiogenesis and cell proliferation, and accelerate epithelization process, so as to prevent wound deepening and achieve the rapid wound healing.
