CAJ | 학술논문

为研究枯草芽孢杆菌 Bs －15对草甘膦的降解作用，以草甘膦异丙胺盐为药剂，对 Bs －15降解草甘膦异丙胺盐的能力进行驯化，并对其培养条件进行优化，测定该菌对草甘膦异丙胺盐的降解率。结果表明， Bs －15可耐受的草甘膦异丙胺盐浓度为40000 mg／L；草甘膦异丙胺盐浓度不高于10000 mg／L，培养基初始pH 值为8．0，接种比例为4％，于35°C、180 r／min 条件下培养60 h，可获得最佳降解，结合实际生产，将培养温度定为30℃；在上述优化条件下，Bs －15对草甘膦异丙胺盐的降解率可达70％左右。
위연구고초아포간균 Bs －15대초감련적강해작용，이초감련이병알염위약제，대 Bs －15강해초감련이병알염적능력진행순화，병대기배양조건진행우화，측정해균대초감련이병알염적강해솔。결과표명， Bs －15가내수적초감련이병알염농도위40000 mg／L；초감련이병알염농도불고우10000 mg／L，배양기초시pH 치위8．0，접충비례위4％，우35°C、180 r／min 조건하배양60 h，가획득최가강해，결합실제생산，장배양온도정위30℃；재상술우화조건하，Bs －15대초감련이병알염적강해솔가체70％좌우。
In order to study the biodegradation of glyphosate by Bacillus subtilis Bs -15,the capacity of Bs -15 to degrade glyphosate isopropylamine was acclimated,the culture conditions were optimized,and the degradation rate of Bs -15 to glyphosate isopropylamine was determined in this paper.The results showed that the maximum concentration that Bs -15 could tolerate was up to 40 000 mg/L.Under the conditions of no more than 10 000 mg/L of glyphosate isopropylamine,8.0 of pH value,4% of inoculation proportion,the op-timal degradation occurred when cultured at 35°C and 180 r/min for 60 hours.The culture temperature was adjusted to 30°C to be more feasible in productivity.Under the above optimum conditions,the degradation rate of Bs -15 to glyphosate isopropylamine could reach about 70%.