CAJ | 학술논문

目的：：探讨不同强度耐力运动对大鼠心肌不同部位胶原蛋白的影响,并研究 MMP-1/TIMP-1的表达变化,为运动性心律失常的发生机制提供实验依据。方法：72只健康成年雄性 SD 大鼠随机分为安静对照组、中等强度运动组和大强度运动组,每组24只。分别进行8周、12周和16周运动,每周训练5天,休息2天,每次1 h。运动8周、12周和16周后,24 h 内处死取材摘取心脏,分离出右心房、右心室和左心室。天狼星红染色观察心肌间质中胶原蛋白的变化并计算胶原容积分数(CVF)。免疫荧光检测Ⅰ型胶原蛋白的含量。West-ern Blot 检测 MMP-1和 TIMP-1的蛋白表达,并计算 MMP-1/TIMP-1的比值。结果：大强度耐力运动提高了右心房和右心室 CVF 和Ⅰ型胶原蛋白的表达(P <0.01),且运动时间越长,增加幅度越大,对左心室 CVF 和Ⅰ型胶原蛋白的表达影响不明显。中等强度运动虽促进了心肌 CVF 和Ⅰ型胶原蛋白的表达,但无统计学意义。蛋白检测发现,16周的大强度运动对右心室 MMP-1表达的影响不明显,却显著提高了右心室 TIMP-1的表达(P <0.01),使得MMP-1/TIMP-1(P <0.05)的比值显著降低。中等强度运动对 MMP-1和 TIMP-1表达的影响不明显。结论：长期大强度耐力运动导致大鼠右心房和右心室胶原容积分数和Ⅰ型胶原蛋白显著增加。12周大强度耐力运动后大鼠右心房发生纤维化,且随运动时间增加,心房纤维化程度增大,右心室的纤维化出现在第16周。心肌纤维化的发生可能与长期大强度耐力运动造成 TIMP-1的表达增加、进而引起 MMP-1/TIMP-1的比例失调有关。
목적：：탐토불동강도내력운동대대서심기불동부위효원단백적영향,병연구 MMP-1/TIMP-1적표체변화,위운동성심률실상적발생궤제제공실험의거。방법：72지건강성년웅성 SD 대서수궤분위안정대조조、중등강도운동조화대강도운동조,매조24지。분별진행8주、12주화16주운동,매주훈련5천,휴식2천,매차1 h。운동8주、12주화16주후,24 h 내처사취재적취심장,분리출우심방、우심실화좌심실。천랑성홍염색관찰심기간질중효원단백적변화병계산효원용적분수(CVF)。면역형광검측Ⅰ형효원단백적함량。West-ern Blot 검측 MMP-1화 TIMP-1적단백표체,병계산 MMP-1/TIMP-1적비치。결과：대강도내력운동제고료우심방화우심실 CVF 화Ⅰ형효원단백적표체(P <0.01),차운동시간월장,증가폭도월대,대좌심실 CVF 화Ⅰ형효원단백적표체영향불명현。중등강도운동수촉진료심기 CVF 화Ⅰ형효원단백적표체,단무통계학의의。단백검측발현,16주적대강도운동대우심실 MMP-1표체적영향불명현,각현저제고료우심실 TIMP-1적표체(P <0.01),사득MMP-1/TIMP-1(P <0.05)적비치현저강저。중등강도운동대 MMP-1화 TIMP-1표체적영향불명현。결론：장기대강도내력운동도치대서우심방화우심실효원용적분수화Ⅰ형효원단백현저증가。12주대강도내력운동후대서우심방발생섬유화,차수운동시간증가,심방섬유화정도증대,우심실적섬유화출현재제16주。심기섬유화적발생가능여장기대강도내력운동조성 TIMP-1적표체증가、진이인기 MMP-1/TIMP-1적비례실조유관。
Objective:To explore whether sustained intensive exercise induces myocardial fibrosis through observing the dyanamics of collagen expression,and discuss the change MMP-1/TIMP-1 expression,thus provide experimental evidence for clarifiying the mechanism of exer-cise- induced arrhythmia.Method:7 2 SD rats were divided into control group (C),moderate intensity group (M)and high instensity group (H )with 2 4 animals in each group.M and H group were conditioned to run 4 ,8 ,and 1 6 weeks,5 days/weeks,1 h/day.Rats were eutha-nized to obtain hearts within 2 4 h after exercise.Right atrium,Right ventricle and Left ventricle were collected.Cardiac collagen were quantified by CVF which is calculateed through by sirius red.Collagen expression were quantified by Immunofluorescence.MMP-1 and TIMP-1 expres-sion were detected by Western Blot.Results:Increasing CVF and collagen expression along time were noted in right atrium and right vetricule by intensity endurance exercise(P <0.01). No differences were observed in left ventricle and M group.In further reserch,enhanced TIMP-1 expressed were noted (P <0 .0 1 ),but only slight increase of MMP-1 in right ventri-cle after 1 6 weeks of intensive exercise.As a result,the ratio of MMP-1/TIMP-1 were de-creased (P < 0 .0 5 ).We could not find difference of MMP-1 and TIMP-1 in M group com-pared to C group.Conclusion:Long-term intensity exercise promotes excessive expression of collagen in right atrium and right ventricle,thus leads to impaired right ventricular founction and myocardial fibrosis.Possible mechanism maybe involve overexpression TIMP-1 ,imbalance of MMP-1/TIMP-1 ratio induced by long-term extensive exercise.