CAJ | 학술논문

目的：建立动物组织中呋喃妥因代谢物1-氨基乙内酰脲的直接竞争酶联免疫(ELISA)检测法,为检测食品中呋喃妥因代谢物残留提供快速检测的方法。方法采用活化酯法制备辣根过氧化物酶标记抗原,并用棋盘法确定酶标抗原和抗体的稀释度,优化反应条件,建立检测呋喃妥因代谢物的直接竞争酶联免疫法,并对其进行方法学评价。结果该方法的线性方程为 Y=-34.723X+158.01(r2=0.9922),线性检测范围为0.177~11.508 ng/mL, IC50为1.291 ng/mL；猪肉、鸡肉、鱼肉的最低检测限分别为0.115、0.113、0.109μg/kg,加标回收率在82.6%~104.7%之间,批内变异系数在3.6%~9.3%之间,批间变异系数在4.3%~12.4%之间。结论本方法快速准确,适用于动物组织中呋喃妥因代谢物的检测。
목적：건립동물조직중부남타인대사물1-안기을내선뇨적직접경쟁매련면역(ELISA)검측법,위검측식품중부남타인대사물잔류제공쾌속검측적방법。방법채용활화지법제비랄근과양화물매표기항원,병용기반법학정매표항원화항체적희석도,우화반응조건,건립검측부남타인대사물적직접경쟁매련면역법,병대기진행방법학평개。결과해방법적선성방정위 Y=-34.723X+158.01(r2=0.9922),선성검측범위위0.177~11.508 ng/mL, IC50위1.291 ng/mL；저육、계육、어육적최저검측한분별위0.115、0.113、0.109μg/kg,가표회수솔재82.6%~104.7%지간,비내변이계수재3.6%~9.3%지간,비간변이계수재4.3%~12.4%지간。결론본방법쾌속준학,괄용우동물조직중부남타인대사물적검측。
ABSTRACT:Objective To develop a direct competitive enzyme-linked immunosorbent assay (ELISA) for the detection of nitrofurantion metabolite in animal tissues. Methods The horseradish peroxidase labeled antigen was prepared by activated ester method. The dilutions of the enzyme labeled antigen and monoclonal antibody were determined by chequerboard titration, and the reaction conditions were optimized to establish the direct competitive enzyme-linked immunoassay for the detection of nitrofurantion metabolite. Then the assay was evaluated. Results The regression equation was Y=-34.723X+158.01(r2=0.9922) with a linear detection ranging from 0.177 to 11.508 ng/mL. The IC50 was 1.291 ng/mL. The limit of detection (LOD) in pork, chicken and fish samples was 0.115, 0.113 and 0.109 μg/kg, respectively. Recoveries were between 82.6%~104.7%when AHD was spiked to samples. The coefficient of variation of intra-and inter-assay was 3.6%~9.3% and 4.3%~12.4%. Conclusion The ELISA method is fast and accurate, and suitable for the detection of nitrofurantion metabolite residue in animal tissues.