CAJ | 학술논문

选用胚乳直链淀粉和支链淀粉含量不同的5个粳稻品种，分析籽粒灌浆过程中异淀粉酶基因在胚乳中的表达特性和酶活性变化及其与淀粉含量间的关系。结果表明，在灌浆过程中胚乳异淀粉酶活性及ISA1和ISA3基因的 m R N A表达量，直链淀粉含量不同的非糯品种都呈先升后降的单峰曲线变化。灌浆中期出现峰值，但糯稻品种呈直线上升的变化趋势，后期的表达量明显高于前期；ISA2基因mRNA表达量自抽穗后10 d起随灌浆进程逐渐下降；在灌浆过程中胚乳ISAs 基因的 mRNA表达量与异淀粉酶活性正相关；ISA1和ISA3基因 mRNA 表达量、异淀粉酶活性及支链淀粉含量呈同步变化。在整个灌浆过程中ISA1基因和ISA1和ISA3基因的表达量明显大于ISA2基因，是灌浆过程中主要表达的基因。
선용배유직련정분화지련정분함량불동적5개갱도품충，분석자립관장과정중이정분매기인재배유중적표체특성화매활성변화급기여정분함량간적관계。결과표명，재관장과정중배유이정분매활성급ISA1화ISA3기인적 m R N A표체량，직련정분함량불동적비나품충도정선승후강적단봉곡선변화。관장중기출현봉치，단나도품충정직선상승적변화추세，후기적표체량명현고우전기；ISA2기인mRNA표체량자추수후10 d기수관장진정축점하강；재관장과정중배유ISAs 기인적 mRNA표체량여이정분매활성정상관；ISA1화ISA3기인 mRNA 표체량、이정분매활성급지련정분함량정동보변화。재정개관장과정중ISA1기인화ISA1화ISA3기인적표체량명현대우ISA2기인，시관장과정중주요표체적기인。
By using five j aponica rice varieties with different amylose and amylopectin contents in endosperm as experimental material,we analyzed the expression levels of ISAs and the activity of isomylase,and their correlation with starch content during grain filling.It turned out that the isomylase activity,the expression levels of ISA1 and ISA3 of tested varieties followed a unimodal curve with peak value appearing during the mid-filling stage except sticky rice,while for the sticky rice,the expression level showed a straight-line growth during grain filling with obvious higher expression in late stage.The expression level of ISA2 was down-regulated ten days after heading.In principle, the expression levels of ISA1 and ISA3 were significantly positively correlated with the enzyme activity,and they both synchronously changed with the content of amylopectin.During grain filling,the expression levels of ISA1 and ISA3 were absolutely higher than that of ISA2,indicating that they were dominated genes expressed during grain filling.