CAJ | 학술논문

通过对95份水稻种质资源进行苗期耐冷性鉴定,筛选出10份苗期耐冷(存活率≥60％)和9份冷敏感的材料(存活率为零),并对水稻冷胁迫响应基因OsSADMC进行了表达分析,发现对照和冷胁迫处理条件下该基因在耐冷品种中的表达量都显著高于冷敏感品种。对耐冷材料丽江新团黑谷(LTH)和冷敏感材料 IR29的基因编码区域进行了克隆和测序分析,在两个品种间鉴定了10个 SNP位点,其中第749碱基处存在的一个碱基变异(C突变为T),从而使丝氨酸变为亮氨酸。根据其中一个 SNP位点设计了OsSADMC的功能标记 SADMC-CAPS1,该标记可以准确地鉴定出19份耐冷性不同水稻种质资源OsSADMC的基因型和耐冷性。
통과대95빈수도충질자원진행묘기내랭성감정,사선출10빈묘기내랭(존활솔≥60％)화9빈랭민감적재료(존활솔위령),병대수도랭협박향응기인OsSADMC진행료표체분석,발현대조화랭협박처리조건하해기인재내랭품충중적표체량도현저고우랭민감품충。대내랭재료려강신단흑곡(LTH)화랭민감재료 IR29적기인편마구역진행료극륭화측서분석,재량개품충간감정료10개 SNP위점,기중제749감기처존재적일개감기변이(C돌변위T),종이사사안산변위량안산。근거기중일개 SNP위점설계료OsSADMC적공능표기 SADMC-CAPS1,해표기가이준학지감정출19빈내랭성불동수도충질자원OsSADMC적기인형화내랭성。
Cold tolerance of 9 5 rice germplasms were screened at seedling stage.Among them,ten cold tolerant lines (survival rate ≥60%)and nine cold sensitive lines (survival rate=0%)were identified.Gene expression analysis of cold stress responsive gene OsSADMC showed that the expression levels of OsSADMC were significantly higher in the cold tolerant lines than those in the cold sensitive lines at both normal and cold stress conditions.Ten SNP loci were identified in the gene coding region between Lijiangxintuanheigu(LTH)and IR29.One amino acid mutation (from serine to leucine)was found between LTH and IR29.A functional marker (SADMC-CAPS1)was developed based on one SNP locus.The allele of OsSADMC carried by ten cold tolerant lines could be distinctly distinguished from that of nine cold sensitive lines by this functional marker.