CAJ | 학술논문

黄曲霉毒素(aflatoxin)是由霉菌黄曲霉和寄生曲霉感染后产生的次生代谢产物，是自然界中毒性最强的化合物之一，被世界卫生组织的癌症研究机构(IARC)划定为I类致癌物质，与肝癌的发生密切相关。黄曲霉毒素很容易污染谷物、坚果、香料、植物油脂、乳类及其制品等多种食品。因此，研究出快速、准确、高效的食品中黄曲霉毒素的检测方法是政府、企业和研究者们一直以来的共同目标。本文综述了近年来基于免疫技术发展的各种快速检测黄曲霉毒素方法，主要包括酶联免疫吸附测定法、胶体金免疫层析法、免疫传感器、免疫亲和柱-高效液相色谱法、免疫荧光法、放射免疫法、时间分辨荧光免疫分析法和量子点探针法的基本原理、适用范围、检测效果、优缺点及国内外应用等方面进展，为今后研发更优的检测黄曲霉素新方法提供借鉴。
황곡매독소(aflatoxin)시유매균황곡매화기생곡매감염후산생적차생대사산물，시자연계중독성최강적화합물지일，피세계위생조직적암증연구궤구(IARC)화정위I류치암물질，여간암적발생밀절상관。황곡매독소흔용역오염곡물、견과、향료、식물유지、유류급기제품등다충식품。인차，연구출쾌속、준학、고효적식품중황곡매독소적검측방법시정부、기업화연구자문일직이래적공동목표。본문종술료근년래기우면역기술발전적각충쾌속검측황곡매독소방법，주요포괄매련면역흡부측정법、효체금면역층석법、면역전감기、면역친화주-고효액상색보법、면역형광법、방사면역법、시간분변형광면역분석법화양자점탐침법적기본원리、괄용범위、검측효과、우결점급국내외응용등방면진전，위금후연발경우적검측황곡매소신방법제공차감。
ABSTRACT:Aflatoxin is secondary metabolites produced by Aspergillus species of fungi. As one of the most toxic compounds in nature, aflatoxin has been classified by the International Agency for Research on Cancer (IARC) as group I carcinogen and is closely related to the development of liver cancer. Cereals, nuts, spices, vegetable oil, milk and their products are the most susceptible commodities to aflatoxin. Therefore, it is a common goal for the government, enterprises and researchers to detect aflatoxin in foodstuffs rapidly, accurately and efficiently. In this paper, the principle, effect, characteristics and application of the methods, including enzyme-linked immunosorbent assay (ELISA), colloidal gold immunoassay, immunosensor assay, immunoaffinity column clean-up and high performance liquid chromatography (HPLC), immunofluorescence-based measurement, radio immunoassay, time resolved fluoroimmunoassay and quantum dots as fluorescent probes assay based on immune technology, were reviewed, so as to provide important references for the development of new superior detection methods of aflatoxin.