中国临床医学
中國臨床醫學
중국림상의학
Chinese Journal of Clinical Medicine
2015年
4期
482-485
,共4页
刘菁%刘学东%赵伟业%王毅%韩秀迪%吕森森%魏风芹%王光艳
劉菁%劉學東%趙偉業%王毅%韓秀迪%呂森森%魏風芹%王光豔
류정%류학동%조위업%왕의%한수적%려삼삼%위풍근%왕광염
慢性阻塞性肺疾病%肺气肿%PM2.5%动物模型
慢性阻塞性肺疾病%肺氣腫%PM2.5%動物模型
만성조새성폐질병%폐기종%PM2.5%동물모형
Chronic obstructive pulmonary disease%Emphysema%PM2.5%Model
目的：：比较吸入 PM2.5颗粒和气管内滴注 PM2.5混悬液两种方法构建大鼠慢性阻塞性肺疾病(COPD)模型的效果。方法：将30只 Wistar 大鼠随机分为对照组(A 组)、PM2.5烟熏组(B 组)及 PM2.5混悬液气管内滴注组(C 组)。观察不同时期大鼠的肺功能、支气管肺泡灌洗液(BALF)中各类细胞计数及肺组织病理切片。结果：与 A 组比较,B 组、C 组大鼠的体质量减轻；潮气量(VT)、呼气峰流速(PEF)减低；BALF 中细胞总数、淋巴细胞数(L)、中性粒细胞数(N)增多,淋巴细胞比例(L%)、中性粒细胞比例(N%)增高,差异均有统计学意义(P <0.05)。病理学观察示,B 组肺泡腔扩大,部分肺泡间隔断裂,肺泡融合,形成肺气肿；气道上皮细胞排列紊乱,部分气道上皮细胞增生,出现炎性细胞浸润并且伴有平滑肌的增生,平均内衬间隔(MLI)较 A 组增加,而平均肺泡数(MAN)较 A 组减少。C 组和 B 组大鼠的病理改变相似,但 C 组病变程度更重,且以急性损伤为主。结论：吸入 PM2.5颗粒较 PM2.5气管内滴注建立的 COPD 模型更合理。
目的：：比較吸入 PM2.5顆粒和氣管內滴註 PM2.5混懸液兩種方法構建大鼠慢性阻塞性肺疾病(COPD)模型的效果。方法：將30隻 Wistar 大鼠隨機分為對照組(A 組)、PM2.5煙熏組(B 組)及 PM2.5混懸液氣管內滴註組(C 組)。觀察不同時期大鼠的肺功能、支氣管肺泡灌洗液(BALF)中各類細胞計數及肺組織病理切片。結果：與 A 組比較,B 組、C 組大鼠的體質量減輕；潮氣量(VT)、呼氣峰流速(PEF)減低；BALF 中細胞總數、淋巴細胞數(L)、中性粒細胞數(N)增多,淋巴細胞比例(L%)、中性粒細胞比例(N%)增高,差異均有統計學意義(P <0.05)。病理學觀察示,B 組肺泡腔擴大,部分肺泡間隔斷裂,肺泡融閤,形成肺氣腫；氣道上皮細胞排列紊亂,部分氣道上皮細胞增生,齣現炎性細胞浸潤併且伴有平滑肌的增生,平均內襯間隔(MLI)較 A 組增加,而平均肺泡數(MAN)較 A 組減少。C 組和 B 組大鼠的病理改變相似,但 C 組病變程度更重,且以急性損傷為主。結論：吸入 PM2.5顆粒較 PM2.5氣管內滴註建立的 COPD 模型更閤理。
목적：：비교흡입 PM2.5과립화기관내적주 PM2.5혼현액량충방법구건대서만성조새성폐질병(COPD)모형적효과。방법：장30지 Wistar 대서수궤분위대조조(A 조)、PM2.5연훈조(B 조)급 PM2.5혼현액기관내적주조(C 조)。관찰불동시기대서적폐공능、지기관폐포관세액(BALF)중각류세포계수급폐조직병리절편。결과：여 A 조비교,B 조、C 조대서적체질량감경；조기량(VT)、호기봉류속(PEF)감저；BALF 중세포총수、림파세포수(L)、중성립세포수(N)증다,림파세포비례(L%)、중성립세포비례(N%)증고,차이균유통계학의의(P <0.05)。병이학관찰시,B 조폐포강확대,부분폐포간격단렬,폐포융합,형성폐기종；기도상피세포배렬문란,부분기도상피세포증생,출현염성세포침윤병차반유평활기적증생,평균내츤간격(MLI)교 A 조증가,이평균폐포수(MAN)교 A 조감소。C 조화 B 조대서적병리개변상사,단 C 조병변정도경중,차이급성손상위주。결론：흡입 PM2.5과립교 PM2.5기관내적주건립적 COPD 모형경합리。
Objective:To compare the effect for establishing rat model of chronic obstructive pulmonary disease(COPD)be-tween two methods,inhalation of PM2.5 and intratracheal instillation of PM2.5 suspension.Methods:A total of 30 adult rats were randomly divided into control group(group A),group fumigated with PM2.5(group B),and group instilled intratracheal-ly with PM2.5 suspension(group C).Then the lung function of rats,the counts of inflammatory cells in bronchoalveolar lavage fluid(BALF),and the pathological sections of lung tissues,were observed on different phases.Results:Compared with that in group A,the body mass in group B or group C decreased.And the tidal volume(VT)and the peak expiratory flow(PEF)de-creased.Furthermore,total cell count,lymphocyte count(L),neutrophil count(N),lymphocyte proportion(L%),and neutro-phil proportion(N%)in BALF increased.All the differences showed statistical significance(all P <0.05).Morphological de-tection showed enlarged alveolar space,disruption of alveolar septa,fusion of alveoli,and formation of emphysema in group B. And it also showed disordered arrangement of airways epithelial cells,partial airway epithelial hyperplasia,inflammatory cell infiltration and proliferation of smooth muscle in group B.Furthermore,mean linear intercept (MLI)and mean alveolar num-ber (MAN)in group B increased,while compared with that in group A.The pathological changes in group C were similar to those in group B.However,the degrees of pathological changes in group C were more severe,and most of them were acute in-juries.Conclusions:Rat model of COPD established by inhalation of PM2.5 was more reasonable than that established by intra-tracheal instillation of PM2.5 suspension.
