食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
Journal of Food Safety & Quality
2015年
9期
3497-3503
,共7页
颜瑛%罗玉彬%王文娟%潘影%万承波%李仕祥
顏瑛%囉玉彬%王文娟%潘影%萬承波%李仕祥
안영%라옥빈%왕문연%반영%만승파%리사상
能力验证%沙门氏菌%菌株分离%菌株鉴定
能力驗證%沙門氏菌%菌株分離%菌株鑒定
능력험증%사문씨균%균주분리%균주감정
proficiency testing%Salmonella%strain isolation%strain identification
目的:为了提升实验室食品中沙门氏菌检测能力,增强实验室竞争能力,本实验室参加了中国食品药品检定研究院组织的沙门氏菌检测能力验证活动。方法依据中华人民共和国国家标准GB 4789.4-2010,采用传统分离方法和血清学鉴定,联合全自动微生物生化鉴定系统(VITEK2-compact)对分离出的疑似菌进行生化鉴定。结果编号为CODE1样品+CODE1奶粉混合样检出纽波特沙门氏菌和科林德尔沙门氏菌, CODE3样品+CODE3奶粉混合样检出维普拉沙门氏菌、鼠伤寒沙门氏菌和埃科沙门氏菌,其余8个样品未检出。结论顺利完成本次能力验证活动。
目的:為瞭提升實驗室食品中沙門氏菌檢測能力,增彊實驗室競爭能力,本實驗室參加瞭中國食品藥品檢定研究院組織的沙門氏菌檢測能力驗證活動。方法依據中華人民共和國國傢標準GB 4789.4-2010,採用傳統分離方法和血清學鑒定,聯閤全自動微生物生化鑒定繫統(VITEK2-compact)對分離齣的疑似菌進行生化鑒定。結果編號為CODE1樣品+CODE1奶粉混閤樣檢齣紐波特沙門氏菌和科林德爾沙門氏菌, CODE3樣品+CODE3奶粉混閤樣檢齣維普拉沙門氏菌、鼠傷寒沙門氏菌和埃科沙門氏菌,其餘8箇樣品未檢齣。結論順利完成本次能力驗證活動。
목적:위료제승실험실식품중사문씨균검측능력,증강실험실경쟁능력,본실험실삼가료중국식품약품검정연구원조직적사문씨균검측능력험증활동。방법의거중화인민공화국국가표준GB 4789.4-2010,채용전통분리방법화혈청학감정,연합전자동미생물생화감정계통(VITEK2-compact)대분리출적의사균진행생화감정。결과편호위CODE1양품+CODE1내분혼합양검출뉴파특사문씨균화과림덕이사문씨균, CODE3양품+CODE3내분혼합양검출유보랍사문씨균、서상한사문씨균화애과사문씨균,기여8개양품미검출。결론순리완성본차능력험증활동。
Objective To strengthen the detection capability of Salmonella in food and improve the competition ability of laboratory, the laboratory participated in proficiency testing on Salmonella detection in foods by National Institutes for Food and Drug Control. Methods Traditional separation and serological identification methods were carried out according to GB 4789.4-2010. The biochemical identification of isolated suspicious colonies was detected by biochemical identification system (VITEK2-compact). Results Salmonella newport and Salmonella colindale were detected in the mixed sample of CODE 1 and CODE 1 powdered milk, Salmonella wipprat, Salmonella typhimurium and Salmonella eko were detected in the mixed sample of CODE 3 and CODE 3 powdered milk, but Salmonella spp. were not detected in other 8 samples. Conclusion The proficiency testing was successfully completed.