食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
Journal of Food Safety & Quality
2015年
9期
3428-3434
,共7页
空肠弯曲菌%聚合酶链反应%食源性致病菌%人畜共患病
空腸彎麯菌%聚閤酶鏈反應%食源性緻病菌%人畜共患病
공장만곡균%취합매련반응%식원성치병균%인축공환병
Campylobacter jejuni%polymerase chain reaction%food-borne pathogenic bacteria%zoonoses
空肠弯曲菌(Campylobacter jejuni)是一种人畜共患病病原菌,可以使人和动物引发多种疾病。目前,检测C. jejuni采用的国标方法是传统的培养法,但C. jejuni培养条件苛刻,且培养法存在操作繁琐、特异性不强、费时等缺点。聚合酶链式反应(polymerase chain reaction, PCR)以其快速、准确、灵敏度高、特异性强的特点,现已广泛应用于C. jejuni的检测,并成为目前快速检测临床与食品中C. jejuni最常用的的方法。本文综述了近年来利用RCR技术,包括常规PCR、多重PCR、巢式PCR、实时荧光定量PCR、PCR-酶联免疫吸附法、最大几率数-PCR、PCR-限制性片段长度多态性、PCR-变性高效液相色谱、PCR-变性梯度凝胶电泳和磁捕获-荧光PCR方法检测C. jejuni的研究进展,并针对这些PCR技术的原理、检测效果、优点和缺点等方面进行了分析比较,为有效控制和预防该菌引起的疾病提供重要信息。
空腸彎麯菌(Campylobacter jejuni)是一種人畜共患病病原菌,可以使人和動物引髮多種疾病。目前,檢測C. jejuni採用的國標方法是傳統的培養法,但C. jejuni培養條件苛刻,且培養法存在操作繁瑣、特異性不彊、費時等缺點。聚閤酶鏈式反應(polymerase chain reaction, PCR)以其快速、準確、靈敏度高、特異性彊的特點,現已廣汎應用于C. jejuni的檢測,併成為目前快速檢測臨床與食品中C. jejuni最常用的的方法。本文綜述瞭近年來利用RCR技術,包括常規PCR、多重PCR、巢式PCR、實時熒光定量PCR、PCR-酶聯免疫吸附法、最大幾率數-PCR、PCR-限製性片段長度多態性、PCR-變性高效液相色譜、PCR-變性梯度凝膠電泳和磁捕穫-熒光PCR方法檢測C. jejuni的研究進展,併針對這些PCR技術的原理、檢測效果、優點和缺點等方麵進行瞭分析比較,為有效控製和預防該菌引起的疾病提供重要信息。
공장만곡균(Campylobacter jejuni)시일충인축공환병병원균,가이사인화동물인발다충질병。목전,검측C. jejuni채용적국표방법시전통적배양법,단C. jejuni배양조건가각,차배양법존재조작번쇄、특이성불강、비시등결점。취합매련식반응(polymerase chain reaction, PCR)이기쾌속、준학、령민도고、특이성강적특점,현이엄범응용우C. jejuni적검측,병성위목전쾌속검측림상여식품중C. jejuni최상용적적방법。본문종술료근년래이용RCR기술,포괄상규PCR、다중PCR、소식PCR、실시형광정량PCR、PCR-매련면역흡부법、최대궤솔수-PCR、PCR-한제성편단장도다태성、PCR-변성고효액상색보、PCR-변성제도응효전영화자포획-형광PCR방법검측C. jejuni적연구진전,병침대저사PCR기술적원리、검측효과、우점화결점등방면진행료분석비교,위유효공제화예방해균인기적질병제공중요신식。
ABSTRACT:As a zoonotic pathogen, Campylobacter jejuni could cause lots of diseases of human and animal. At present, plate-culture method is used to detect C. jejuni as the national standard. But the cultivation conditions of C. jejuni are very strict, and the plate-culture method has the disadvantages such as complicated operation, specificity, and time consuming. Polymerase chain reaction, which is excellent for the rapid, accurate, high sensitivity and specificity, has been widely used in the detection of C. jejuni, and become the most common method for the rapid detection of C. jejuni in clinical and food samples. The recent research progress was summarized in this paper and the principle, effect and characteristics among conventional PCR, multiplex PCR, nested PCR, real-time PCR, PCR-ELISA, MPN (most probable number)-PCR, PCR-RFLP (restriction fragment length polymorphism), PCR-DHPLC (denaturing high performance liquid chromatography), PCR-DGGE (denaturing gradient gel electrophoresis) and IMC-FPCR (immunomagnetic capture fluorescent PCR) were also compared and analyzed for providing important information for the effective prevention and control of diseases caused by C. jejuni.
