中国临床医学
中國臨床醫學
중국림상의학
Chinese Journal of Clinical Medicine
2015年
4期
471-474
,共4页
肝细胞癌%microRNA-148b%总生存期%预后
肝細胞癌%microRNA-148b%總生存期%預後
간세포암%microRNA-148b%총생존기%예후
Hepatocellular carcinoma%microRNA-148b%Overall survival%Prognosis
目的：：探讨肝细胞癌(hepatocellular carcinoma,HCC)组织中 microRNA-148b(miR-148b)的表达与 HCC 预后的关系。方法：从上海中医药大学附属曙光医院冰冻组织样本库中调取2007—2010年手术切除的 HCC 组织及相应癌旁组织标本,采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)法检测组织标本中 miR-148b 的表达情况,结合临床病理指标及生存资料,分析 miR-148b 表达改变的临床意义。结果：与癌旁组织比较,HCC 组织中 miR-148b表达水平显著降低(P <0.01)。miR-148b 低表达与 HCC 血管侵犯及 TNM 分期等恶性临床病理指标相关。Kaplan-Meier 生存曲线分析显示,miR-148b 低表达组患者总生存率较低。结论：人 HCC 组织中 miR-148b 表达下降,且与 HCC 不良的临床预后有关。
目的：：探討肝細胞癌(hepatocellular carcinoma,HCC)組織中 microRNA-148b(miR-148b)的錶達與 HCC 預後的關繫。方法：從上海中醫藥大學附屬曙光醫院冰凍組織樣本庫中調取2007—2010年手術切除的 HCC 組織及相應癌徬組織標本,採用實時熒光定量聚閤酶鏈反應(real-time quantitative polymerase chain reaction,RT-qPCR)法檢測組織標本中 miR-148b 的錶達情況,結閤臨床病理指標及生存資料,分析 miR-148b 錶達改變的臨床意義。結果：與癌徬組織比較,HCC 組織中 miR-148b錶達水平顯著降低(P <0.01)。miR-148b 低錶達與 HCC 血管侵犯及 TNM 分期等噁性臨床病理指標相關。Kaplan-Meier 生存麯線分析顯示,miR-148b 低錶達組患者總生存率較低。結論：人 HCC 組織中 miR-148b 錶達下降,且與 HCC 不良的臨床預後有關。
목적：：탐토간세포암(hepatocellular carcinoma,HCC)조직중 microRNA-148b(miR-148b)적표체여 HCC 예후적관계。방법：종상해중의약대학부속서광의원빙동조직양본고중조취2007—2010년수술절제적 HCC 조직급상응암방조직표본,채용실시형광정량취합매련반응(real-time quantitative polymerase chain reaction,RT-qPCR)법검측조직표본중 miR-148b 적표체정황,결합림상병리지표급생존자료,분석 miR-148b 표체개변적림상의의。결과：여암방조직비교,HCC 조직중 miR-148b표체수평현저강저(P <0.01)。miR-148b 저표체여 HCC 혈관침범급 TNM 분기등악성림상병리지표상관。Kaplan-Meier 생존곡선분석현시,miR-148b 저표체조환자총생존솔교저。결론：인 HCC 조직중 miR-148b 표체하강,차여 HCC 불량적림상예후유관。
Objective:To investigate the relationship between the expression of microRNA-148b(miR-148b)in human hepato-cellular carcinoma (HCC)and the prognosis of HCC.Methods:The specimens of HCC tissue and para-carcinoma tissue ac-quired by surgical resection during 2007 and 2010 were collected from frozen tissue specimen library of Shuguang Hospital Af-filiated to Shanghai University of Traditional Chinese Medicine.The expression of miR-148b in tissue specimen was detected by real-time quantitative polymerase chain reaction (RT-qPCR).The clinical significance of expression change of miR-148b was analyzed by considering the clinical pathological indicators and survival data.Results:The expression level of miR-148b in HCC was significantly lower than that in para-tumor tissues(P <0.01).Low expression of miR-148b was associated with HCC ma-lignant clinical pathological indicators such as vascular invasion and TNM stage.Kaplan-Meier survival curve analysis showed that the patients with low expression of miR-148b had poor overall survival rate.Conclusions:The expression of miR-148b de-creases in human HCC tissue.And it is associated with the poor clinical prognosis.
