CAJ | 학술논문

目的：探讨电针治疗脑卒中后认知障碍的可能机制。方法45只Sprague-Dawley雄性大鼠随机分为假手术组(n=15)、模型组(n=15)和电针组(n=15)。模型组、电针组用线栓法制备左侧局灶性脑缺血1.5 h再灌注模型。电针组电针神庭、百会穴7 d。采用Morris水迷宫检测大鼠学习记忆能力；Longa评分法观察大鼠神经功能缺损情况；HE染色观察大鼠海马神经元形态结构变化；免疫印迹法检测大鼠缺血侧海马组织中脑源性神经营养因子(BDNF)、神经营养素受体p75(p75NTR)蛋白表达。结果与模型组相比，电针组逃避潜伏期缩短(P<0.05)，穿越平台次数显著增加(P<0.001)；神经功能缺损评分明显减少(P<0.01)；缺血侧海马区细胞损伤较轻，BDNF表达明显升高(P<0.01)，p75NTR蛋白表达明显降低(P<0.01)。结论电针能够改善脑缺血再灌注大鼠学习记忆能力，其机制可能与上调大鼠海马组织中BDNF蛋白和下调p75NTR蛋白表达水平有关。
목적：탐토전침치료뇌졸중후인지장애적가능궤제。방법45지Sprague-Dawley웅성대서수궤분위가수술조(n=15)、모형조(n=15)화전침조(n=15)。모형조、전침조용선전법제비좌측국조성뇌결혈1.5 h재관주모형。전침조전침신정、백회혈7 d。채용Morris수미궁검측대서학습기억능력；Longa평분법관찰대서신경공능결손정황；HE염색관찰대서해마신경원형태결구변화；면역인적법검측대서결혈측해마조직중뇌원성신경영양인자(BDNF)、신경영양소수체p75(p75NTR)단백표체。결과여모형조상비，전침조도피잠복기축단(P<0.05)，천월평태차수현저증가(P<0.001)；신경공능결손평분명현감소(P<0.01)；결혈측해마구세포손상교경，BDNF표체명현승고(P<0.01)，p75NTR단백표체명현강저(P<0.01)。결론전침능구개선뇌결혈재관주대서학습기억능력，기궤제가능여상조대서해마조직중BDNF단백화하조p75NTR단백표체수평유관。
Objective To observe the effects of electroacupuncture on learning and memory, and discuss the therapeutic mechanism. Methods 45 Sprague-Dawley rats were randomly divided into sham group (n=15), model group (n=15) and electroacupuncture group (n=15). The latter 2 groups were modeled with middle cerebral artery occlusion for 1.5 h and reperfusion. The rats of electroacupuncture group received electroacupuncture at Shenting (DU24) and Baihui (DU20) for 7 days. Learning and memory ability was tested with Morris water maze. Neurologic impairment was assessed with Longa's score. Their hippocampus were observed under HE staining and the level of brain-derived neurotrophic factor (BDNF) and p75 neurotrophin receptor (p75NTR) protein were determined with Western blotting. Results Compared with the model group, the latency of water maze decreased and the times crossing the platform increased in electroacupuncture group (P<0.05), while the Longa's score significantly reduced (P<0.05), and the lesion of nerve cells were alleviated, with the decrease of p75NTR and increase of BDNF in the ischemic hippocampus (P<0.01). Conclusion Electroacupuncture can improve the learning and mem-ory of cerebral ischemia-reperfusion rats, which may relate with up-regulating BDNF and down-regulating p75NTR in hippocampus.