CAJ | 학술논문

于2013－2014连续两年对云南主要水稻种植区的水稻病毒病进行了调查，发现当前为害云南水稻的病毒病主要有水稻条纹叶枯病、南方水稻黑条矮缩病、水稻矮缩病和水稻齿叶矮缩病.其中，条纹叶枯病是当前为害云南水稻最严重的病毒病，部分田块最高发病率可达15．0％；其次是水稻矮缩病，调查田块最高发病率达10．0％；南方水稻黑条矮缩病的平均发病率达5．3％；水稻齿叶矮缩病只在局部地区发生且发病率都不高.对云南当前发生较普遍的 RSV 不同分离物 RNA3基树，所分析的 RSV分离物均可被划分为两个组，部分云南分离物和云南以外的中国、日本、韩国分离物聚在Ⅰ组，其余绝大多数云南分离物单独聚在Ⅱ组.不同RSV分离物的IR3和IR4长度差异较大，按每10个核苷酸的长度差异进行划分，均可划分为 A、B、C三种不同的类型.部分 RSV云南分离物 IR3、IR4中有17－21 nt的序列与其水稻、小麦和大麦等寄主的部分序列相同，这可能是病毒在进化过程中与寄主植物发生了重组.云南 RSV分离物的 IR3、IR4分子变异较大，可能与其特殊的地理、生态条件有一定的相关性.
우2013－2014련속량년대운남주요수도충식구적수도병독병진행료조사，발현당전위해운남수도적병독병주요유수도조문협고병、남방수도흑조왜축병、수도왜축병화수도치협왜축병.기중，조문협고병시당전위해운남수도최엄중적병독병，부분전괴최고발병솔가체15．0％；기차시수도왜축병，조사전괴최고발병솔체10．0％；남방수도흑조왜축병적평균발병솔체5．3％；수도치협왜축병지재국부지구발생차발병솔도불고.대운남당전발생교보편적 RSV 불동분리물 RNA3기수，소분석적 RSV분리물균가피화분위량개조，부분운남분리물화운남이외적중국、일본、한국분리물취재Ⅰ조，기여절대다수운남분리물단독취재Ⅱ조.불동RSV분리물적IR3화IR4장도차이교대，안매10개핵감산적장도차이진행화분，균가화분위 A、B、C삼충불동적류형.부분 RSV운남분리물 IR3、IR4중유17－21 nt적서렬여기수도、소맥화대맥등기주적부분서렬상동，저가능시병독재진화과정중여기주식물발생료중조.운남 RSV분리물적 IR3、IR4분자변이교대，가능여기특수적지리、생태조건유일정적상관성.
Viral disease investigation in main rice planting areas in Yunnan Province from 2013 to 2014 showed that rice stripe disease,southern rice black-streaked dwarf disease,rice dwarf disease and rice ragged stunt disease were the four major viral diseases threaten rice production in Yunnan Province.Among them,rice stripe disease remained widely distributed in Yunnan with the incidence up to 1 5 .0% in some fields followed by rice dwarf disease occurred in some areas with the highest incidence of 10.0%;and the average incidence of southern rice black-streaked dwarf disease was 5 .3%;rice ragged stunt disease occurred with a low incidence.Molecular variation in rice stripe virus (RSV)isolates was analyzed based on the nucleotide sequence of intergenic region (IR)of RNA3 and RNA4.Phylogenetic analysis showed that all of Yunnan RSV isolates could be divided into two distinct clusters.The isolates from Japan,Korea and other provinces of China belonged to groupⅠ,whereas Yunnan isolates fell into both of the two groups and most of them form a distinct Yunnan population of group Ⅱ.The length of IR3 and IR4 varied with RSV isolates,which could be divided into three types of A,B and C according to the difference in the length per 10 nucleotides.Recombination might be occurred during the evolutionary process of RSV since 1 7 to 2 1 nucleotides of IR3 and IR4 of some Yunnan isolates shared some identical sequence with their host plants,rice,wheat or barley.We supposed that the high molecular variation of IR3 and IR4 in Yunnan isolates might result from the special geographical and ecological conditions in Yunnan Province.