中华实用儿科临床杂志
中華實用兒科臨床雜誌
중화실용인과림상잡지
Journal of Applied Clinical Pediatrics
2015年
15期
1161-1163
,共3页
人横纹肌肉瘤RD细胞%苦参碱%X连锁凋亡抑制蛋白
人橫紋肌肉瘤RD細胞%苦參堿%X連鎖凋亡抑製蛋白
인횡문기육류RD세포%고삼감%X련쇄조망억제단백
Human rhabdomyosarcoma RD cell%Matrine%X-linked inhibitor of apoptosis protein
目的 探讨苦参碱对体外培养的人横纹肌肉瘤RD细胞中X连锁凋亡抑制蛋白(XIAP)表达的影响.方法 体外培养人横纹肌肉瘤RD细胞并分为对照组及0.5 g/L、1.0 g/L、1.5 g/L苦参碱干预组.采用四甲基偶氮唑蓝比色法及流式细胞术分别检测RD细胞在不同质量浓度苦参碱作用下的细胞增殖抑制率及凋亡率的变化,反转录PCR法检测RD细胞中XIAP mRNA的表达.结果 不同质量浓度苦参碱干预组及对照组中RD细胞的增殖抑制率[0.5 g/L苦参碱干预组:(15.84±2.58)％、1.0 g/L苦参碱干预组:(23.13±4.19)％、1.5 g/L苦参碱干预组:(30.32±3.02)％、对照组:(8.92±1.23)％]、凋亡率[0.5 g/L苦参碱干预组:(12.33±1.15)％、1.0 g/L苦参碱干预组:(16.67±0.99)％、1.5 g/L苦参碱干预组:(25.33±1.91)％、对照组:(9.47±0.96)％]及XIAP mRNA表达(0.5 g/L苦参碱干预组:0.633 ±0.046、1.0 g/L苦参碱干预组:0.441 ±0.055、1.5 g/L苦参碱干预组:0.326±0.065、对照组:0.794±0.029)均有差异,且差异有统计学意义(F=14.15、83.37、50.57,P均＜0.01).随着苦参碱质量浓度的增加,RD细胞的增殖抑制率及凋亡率明显增加,而XIAP mRNA的表达明显降低,且呈剂量依赖性.结论 苦参碱对人横纹肌肉瘤RD细胞具有抑制增殖及诱导凋亡的作用,且该作用可能与下调凋亡抑制基因XIAP的表达有关.
目的 探討苦參堿對體外培養的人橫紋肌肉瘤RD細胞中X連鎖凋亡抑製蛋白(XIAP)錶達的影響.方法 體外培養人橫紋肌肉瘤RD細胞併分為對照組及0.5 g/L、1.0 g/L、1.5 g/L苦參堿榦預組.採用四甲基偶氮唑藍比色法及流式細胞術分彆檢測RD細胞在不同質量濃度苦參堿作用下的細胞增殖抑製率及凋亡率的變化,反轉錄PCR法檢測RD細胞中XIAP mRNA的錶達.結果 不同質量濃度苦參堿榦預組及對照組中RD細胞的增殖抑製率[0.5 g/L苦參堿榦預組:(15.84±2.58)％、1.0 g/L苦參堿榦預組:(23.13±4.19)％、1.5 g/L苦參堿榦預組:(30.32±3.02)％、對照組:(8.92±1.23)％]、凋亡率[0.5 g/L苦參堿榦預組:(12.33±1.15)％、1.0 g/L苦參堿榦預組:(16.67±0.99)％、1.5 g/L苦參堿榦預組:(25.33±1.91)％、對照組:(9.47±0.96)％]及XIAP mRNA錶達(0.5 g/L苦參堿榦預組:0.633 ±0.046、1.0 g/L苦參堿榦預組:0.441 ±0.055、1.5 g/L苦參堿榦預組:0.326±0.065、對照組:0.794±0.029)均有差異,且差異有統計學意義(F=14.15、83.37、50.57,P均＜0.01).隨著苦參堿質量濃度的增加,RD細胞的增殖抑製率及凋亡率明顯增加,而XIAP mRNA的錶達明顯降低,且呈劑量依賴性.結論 苦參堿對人橫紋肌肉瘤RD細胞具有抑製增殖及誘導凋亡的作用,且該作用可能與下調凋亡抑製基因XIAP的錶達有關.
목적 탐토고삼감대체외배양적인횡문기육류RD세포중X련쇄조망억제단백(XIAP)표체적영향.방법 체외배양인횡문기육류RD세포병분위대조조급0.5 g/L、1.0 g/L、1.5 g/L고삼감간예조.채용사갑기우담서람비색법급류식세포술분별검측RD세포재불동질량농도고삼감작용하적세포증식억제솔급조망솔적변화,반전록PCR법검측RD세포중XIAP mRNA적표체.결과 불동질량농도고삼감간예조급대조조중RD세포적증식억제솔[0.5 g/L고삼감간예조:(15.84±2.58)％、1.0 g/L고삼감간예조:(23.13±4.19)％、1.5 g/L고삼감간예조:(30.32±3.02)％、대조조:(8.92±1.23)％]、조망솔[0.5 g/L고삼감간예조:(12.33±1.15)％、1.0 g/L고삼감간예조:(16.67±0.99)％、1.5 g/L고삼감간예조:(25.33±1.91)％、대조조:(9.47±0.96)％]급XIAP mRNA표체(0.5 g/L고삼감간예조:0.633 ±0.046、1.0 g/L고삼감간예조:0.441 ±0.055、1.5 g/L고삼감간예조:0.326±0.065、대조조:0.794±0.029)균유차이,차차이유통계학의의(F=14.15、83.37、50.57,P균＜0.01).수착고삼감질량농도적증가,RD세포적증식억제솔급조망솔명현증가,이XIAP mRNA적표체명현강저,차정제량의뢰성.결론 고삼감대인횡문기육류RD세포구유억제증식급유도조망적작용,차해작용가능여하조조망억제기인XIAP적표체유관.
Objective To investigate the effect of Matrine on the expression of X-linked inhibitor of apoptosis protein (XIAP) in human rhabdomyosarcoma RD cells in vitro.Methods Cultured human rbabdomyosarcoma RD cells were divided into Matrine intervention groups (0.5 g/L,1.0 g/L and 1.5 g/L) and a control group.The proliferation-inhibition rates in RD cells treated with different concentrations of matrine were detected by methylthiazolyl blue colorimetric assay.Flow cytometry analysis was performed for the apoptosis rates of RD cells.Reverse transcription-polymerase chain reaction analysis was used to measure the XIAP mRNA expression.Results There was a significant difference in the proliferation-inhibition rates [0.5 g/L Matrine group:(15.84 ± 2.58)％,1.0 g/L Matrine group:(23.13 ±4.19)％,1.5 g/L Matrine group:(30.32 ±3.02)％,and the control group:(8.92 ± 1.23)％];apoptotic rates [0.5 g/L Matrine group:(12.33 ± 1.15)％,1.0 g/L Matrine group:(16.67 ± 0.99)％,1.5 g/L Matrine group:(25.33 ± 1.91)％,and the control group:(9.47 ± 0.96)％];XIAP mRNA expression(0.5 g/L Matrine group:0.633 ± 0.046,1.0 g/L Matrine group:0.441 ± 0.055,1.5 g/L Matrine group:0.326 ± 0.065,control group:0.794 ±0.029)in RD cells among 0.5 g/L,1.0 g/L,1.5 g/L Matrine groups and the control group (F =14.15,83.37,50.57,all P ＜ 0.05).The proliferation-inhibition and apoptotic rates in RD cells were gradually increased with the increasing Matrine concentration.The expression of XIAP mRNA was significantly decreased in different Matrine groups compared with the control group,exhibiting a dose-dependent manner.Conclusions Matrine can inhibit the proliferation of RD cells and induce the apoptosis in a dose-dependent manner,which may be related to the down-regulated XIAP mRNA.
