CAJ | 학술논문

目的：探讨决明子乙醇提取物( semen cassiae extract,SCE)对高脂高糖诱导非酒精性脂肪肝大鼠血糖、血脂、胰岛素抵抗及肝功能和肝细胞脂肪变性病理改变以及氧化应激—糖基化作用。方法 SD大鼠72只,雌雄各半,随机分成正常对照组(12只)和模型组(60只)。正常对照组给予普通饲料,饮用蒸馏水；模型组大鼠给予高脂饲料,饮用10%果糖水。饲养至第6周末,将模型组大鼠随机分为模型对照组(蒸馏水10 mL/kg)、二甲双胍组(0．2 g/kg)、决明子乙醇提取物( SCE)高(2 g/kg)、中(1 g/kg)、低(0．5 g/kg)剂量组。连续灌胃给药4周后,测定大鼠血清超氧化物歧化酶( superoxide dismutase,SOD)、谷胱甘肽过氧化物酶( glutathione peroxidase,GSH-Px)、一氧化氮合成酶( nitric oxide synthase, NOS )活性,测定血清一氧化氮( nitric monoxide, NO )、丙二醛( malondialdehyde,MDA)、果糖胺( fructosamine, FMN )、晚期糖基化终产物( advanced glycation end products,AGEs)、葡萄糖含量( fasting blood glucose,FBG),血清胰岛素水平( insulin,INS)及胰岛素敏感度( insulin sensitivity index,ISI)。测定大鼠肝脏组织SOD、NOS活性及NO、MDA含量。结果与正常对照组相比,模型组大鼠血清GSP-Px、SOD和NOS活性降低,NO、MDA、FMN、AGEs、FBG含量升高,INS水平升高、ISI下降；模型组大鼠肝脏组织中SOD和NOS活性降低,NO和MDA含量明显升高(P<0．01)。 SCE和二甲双胍处理4周后,明显提高模型大鼠血清GSP-Px、SOD和NOS活性,降低NO、MDA、FMN、AGEs、FBG水平,并降低INS水平,上调ISI；同时,明显提高肝组织中SOD和NOS活性,降低NO、MDA含量(P<0．01,P<0．05),尤以SCE高剂量组作用更为明显。结论决明子提取物的护肝作用,与其拮抗胰岛素抵抗、增强抗氧化能力以及抑制氧化糖基化反应有关。
목적：탐토결명자을순제취물( semen cassiae extract,SCE)대고지고당유도비주정성지방간대서혈당、혈지、이도소저항급간공능화간세포지방변성병리개변이급양화응격—당기화작용。방법 SD대서72지,자웅각반,수궤분성정상대조조(12지)화모형조(60지)。정상대조조급여보통사료,음용증류수；모형조대서급여고지사료,음용10%과당수。사양지제6주말,장모형조대서수궤분위모형대조조(증류수10 mL/kg)、이갑쌍고조(0．2 g/kg)、결명자을순제취물( SCE)고(2 g/kg)、중(1 g/kg)、저(0．5 g/kg)제량조。련속관위급약4주후,측정대서혈청초양화물기화매( superoxide dismutase,SOD)、곡광감태과양화물매( glutathione peroxidase,GSH-Px)、일양화담합성매( nitric oxide synthase, NOS )활성,측정혈청일양화담( nitric monoxide, NO )、병이철( malondialdehyde,MDA)、과당알( fructosamine, FMN )、만기당기화종산물( advanced glycation end products,AGEs)、포도당함량( fasting blood glucose,FBG),혈청이도소수평( insulin,INS)급이도소민감도( insulin sensitivity index,ISI)。측정대서간장조직SOD、NOS활성급NO、MDA함량。결과여정상대조조상비,모형조대서혈청GSP-Px、SOD화NOS활성강저,NO、MDA、FMN、AGEs、FBG함량승고,INS수평승고、ISI하강；모형조대서간장조직중SOD화NOS활성강저,NO화MDA함량명현승고(P<0．01)。 SCE화이갑쌍고처리4주후,명현제고모형대서혈청GSP-Px、SOD화NOS활성,강저NO、MDA、FMN、AGEs、FBG수평,병강저INS수평,상조ISI；동시,명현제고간조직중SOD화NOS활성,강저NO、MDA함량(P<0．01,P<0．05),우이SCE고제량조작용경위명현。결론결명자제취물적호간작용,여기길항이도소저항、증강항양화능력이급억제양화당기화반응유관。
Objective To investigate the pathological changes of blood glucose, serum lipid, insulin resistance, liver function, liver cell denaturalization, and the oxidative-glycation effects of Semen Cassiae Extract( SCE) in rats with nonalcoholic fatty liver disease ( NAFLD) . Methods 72 SD rats were randomly divided into normal group (12) and model group (60). The normal rats were raised by standard animal diet, while the model rats were fed with high-fat diet and 10% fructose for 6 weeks. Model rats were randomly divided into 5 groups, including fatty liver group, metformin group (0. 2g·kg-1 ), SCE high-dose group (2g·kg-1), middle-dose group(1g·kg-1) and low-dose group (0. 5g·kg-1). All rats were treated for 4 weeks with drugs. The contents or activities of SOD, GSP-Px, NOS, NO, MDA, FMN, AGEs, FBG, INS in the serum and contents or activities of SOD, NOS, NO, MDA in the liver tissue were respectively measured, and ISI was counted. Results Compared with normal rats, the contents of or activities NOS, NO, MDA, FMN, AGEs, FBG, INS in the serum and contents or activities of NOS, NO, MDA in the liver tissue were increased ( P<0. 01 ) , but the activities of SOD, GSP-Px and ISI were decreased ( P<0. 01 ) in model rats. After SCE treatment for 4 weeks, compared with model rats, The contents or activities of SOD, GSP-Px, NOS, NO, MDA, FMN, AGEs, FBG, INS in the serum and contents or activities of NOS, NO, MDA in the liver tissue were decreased respectively, but the activities of SOD, GSP-Px and ISI were increased. Conclusion SCE possess hepatoprotective effect on structures or functions by antagonizing insulin resistance, enhancing the antioxidant capacity and inhibiting oxidative-glycation in rats with NAFLD.