CAJ | 학술논문

目的：探讨牡丹皮对内毒素( lipopolysaccharide ,LPS)诱导的大鼠急性肺损伤( acute lung injury,ALI)的治疗作用,并探讨其可能机制。方法60只wistar大鼠随机分为生理盐水对照组、模型组、地塞米松组、治疗组及预防组,每组12只,通过向气管内滴注LPS建立大鼠ALI模型。观察造模12小时后的肺泡灌洗液中多形核白细胞( polymorphonuclear leukocytes,PMN)百分比、蛋白浓度、肿瘤坏死因子(tumor necrosis factor-alpha,TNF-α)及白细胞介素-6(interleukin-6,IL-6)水平和肺组织的病理形态学变化。结果牡丹皮、地塞米松能够有效地降低 PMN 百分比和蛋白浓度(P<0．01),下调TNF-α、IL-6的水平﹙P<0．05或P<0．01),显著减轻肺泡结构破坏、肺水肿和炎症细胞浸润等病变。结论牡丹皮能够抑制ALI时的炎症反应,减轻肺部炎症损伤,从而对肺起到保护作用。
목적：탐토모단피대내독소( lipopolysaccharide ,LPS)유도적대서급성폐손상( acute lung injury,ALI)적치료작용,병탐토기가능궤제。방법60지wistar대서수궤분위생리염수대조조、모형조、지새미송조、치료조급예방조,매조12지,통과향기관내적주LPS건립대서ALI모형。관찰조모12소시후적폐포관세액중다형핵백세포( polymorphonuclear leukocytes,PMN)백분비、단백농도、종류배사인자(tumor necrosis factor-alpha,TNF-α)급백세포개소-6(interleukin-6,IL-6)수평화폐조직적병리형태학변화。결과모단피、지새미송능구유효지강저 PMN 백분비화단백농도(P<0．01),하조TNF-α、IL-6적수평﹙P<0．05혹P<0．01),현저감경폐포결구파배、폐수종화염증세포침윤등병변。결론모단피능구억제ALI시적염증반응,감경폐부염증손상,종이대폐기도보호작용。
Objective To investigate the effects of Moutan cortexon lipopolysaccharide-induced acute lung injury (ALI) in rats,and explore the underlying mechanism against ALI. Methods 55 wistar rats were randomly divided into control group, model group,positive drug group,treatment group and prevention group,the animal model of ALI was established by intratracheal administration oflipopolysaccharide. The percentage of neutrophils﹑the concentration of protein exudation﹑cytokine ( TNF-α、IL-6 ) levels in bronchoalveolar lavage fluid ( BALF ) and histopathological changes in lung tissue were observed 12 h after LPS administration. Results Moutan cortex and dexamethasone can effectively reduce the PMN percentage and protein concentration(P<0. 01), lower the level of TNF-α﹑IL-6 (P<0. 05 or P<0. 01) ,significantly improve the alveolar structure damage ﹑ pulmonary edema and inflammatory cells infiltration, etc. Conclusion Moutan cortex can protect lung through inhibiting the inflammation reaction of ALI and alleviating pulmonary inflammation.