中华实用儿科临床杂志
中華實用兒科臨床雜誌
중화실용인과림상잡지
Journal of Applied Clinical Pediatrics
2015年
15期
1135-1138
,共4页
急性髓细胞性白血病%Toll样受体4%髓源抑制性细胞
急性髓細胞性白血病%Toll樣受體4%髓源抑製性細胞
급성수세포성백혈병%Toll양수체4%수원억제성세포
Acute myeloid leukemia%Toll-like receptor 4%Myeloid derived suppressor cells
目的 探讨儿童急性髓细胞性白血病(AML)骨髓细胞中Toll样受体4(TLR4)与髓源抑制性细胞(M DSC)的表达及其与AML临床分型、化疗效果及预后的关系.方法 收集2013年6月至2014年3月期间在武汉大学人民医院确诊的AML患儿29例.其中临床低危组11例、中危组10例、高危组8例;另选取17例非血液系统疾病作为对照组.采用反转录-聚合酶链反应(RT-PCR)、Western blot、免疫组织化学、流式细胞仪等方法检测29例AML患儿骨髓细胞TLR4及MDSC的表达.结果 初治组中TLR4 mRNA和蛋白的表达高于完全缓解组,差异均有统计学意义(t=3.092、3.393,P均＜0.05),难治复发组TLR4 mRNA和蛋白的表达显著高于完全缓解组,差异均有统计学意义(t=4.013、4.279,P均＜0.05).初治组、完全缓解组及难治复发组MDSC阳性表达率分别为(29.77±1.39)％、(5.19±0.65)％、(38.62±3.54)％,与对照组[(1.32±0.27)％]相比,差异均有统计学意义(P均＜0.05).初治组、难治复发组及完全缓解组TLR4与MDSC的阳性表达率明显高于对照组,差异有统计学意义(初治组TLR4:t=3.559,P＜0.05;MDSC:t=3.727,P＜0.05;难治复发组TLR4:t=4.043,P＜0.05;MDSC:t=4.125,P＜0.05;完全缓解组TLR4:t=2.798,P＜O.05;MDSC:t=3.469,P ＜0.05).Pearson等级相关分析显示TLR4与MDSC表达呈正相关(r=0.673,P＜0.01).结论 TLR4和MDSC对于AML的发生、发展、疗效及预后密切相关,且两者在AML的发生、发展过程中发挥重要协同作用.
目的 探討兒童急性髓細胞性白血病(AML)骨髓細胞中Toll樣受體4(TLR4)與髓源抑製性細胞(M DSC)的錶達及其與AML臨床分型、化療效果及預後的關繫.方法 收集2013年6月至2014年3月期間在武漢大學人民醫院確診的AML患兒29例.其中臨床低危組11例、中危組10例、高危組8例;另選取17例非血液繫統疾病作為對照組.採用反轉錄-聚閤酶鏈反應(RT-PCR)、Western blot、免疫組織化學、流式細胞儀等方法檢測29例AML患兒骨髓細胞TLR4及MDSC的錶達.結果 初治組中TLR4 mRNA和蛋白的錶達高于完全緩解組,差異均有統計學意義(t=3.092、3.393,P均＜0.05),難治複髮組TLR4 mRNA和蛋白的錶達顯著高于完全緩解組,差異均有統計學意義(t=4.013、4.279,P均＜0.05).初治組、完全緩解組及難治複髮組MDSC暘性錶達率分彆為(29.77±1.39)％、(5.19±0.65)％、(38.62±3.54)％,與對照組[(1.32±0.27)％]相比,差異均有統計學意義(P均＜0.05).初治組、難治複髮組及完全緩解組TLR4與MDSC的暘性錶達率明顯高于對照組,差異有統計學意義(初治組TLR4:t=3.559,P＜0.05;MDSC:t=3.727,P＜0.05;難治複髮組TLR4:t=4.043,P＜0.05;MDSC:t=4.125,P＜0.05;完全緩解組TLR4:t=2.798,P＜O.05;MDSC:t=3.469,P ＜0.05).Pearson等級相關分析顯示TLR4與MDSC錶達呈正相關(r=0.673,P＜0.01).結論 TLR4和MDSC對于AML的髮生、髮展、療效及預後密切相關,且兩者在AML的髮生、髮展過程中髮揮重要協同作用.
목적 탐토인동급성수세포성백혈병(AML)골수세포중Toll양수체4(TLR4)여수원억제성세포(M DSC)적표체급기여AML림상분형、화료효과급예후적관계.방법 수집2013년6월지2014년3월기간재무한대학인민의원학진적AML환인29례.기중림상저위조11례、중위조10례、고위조8례;령선취17례비혈액계통질병작위대조조.채용반전록-취합매련반응(RT-PCR)、Western blot、면역조직화학、류식세포의등방법검측29례AML환인골수세포TLR4급MDSC적표체.결과 초치조중TLR4 mRNA화단백적표체고우완전완해조,차이균유통계학의의(t=3.092、3.393,P균＜0.05),난치복발조TLR4 mRNA화단백적표체현저고우완전완해조,차이균유통계학의의(t=4.013、4.279,P균＜0.05).초치조、완전완해조급난치복발조MDSC양성표체솔분별위(29.77±1.39)％、(5.19±0.65)％、(38.62±3.54)％,여대조조[(1.32±0.27)％]상비,차이균유통계학의의(P균＜0.05).초치조、난치복발조급완전완해조TLR4여MDSC적양성표체솔명현고우대조조,차이유통계학의의(초치조TLR4:t=3.559,P＜0.05;MDSC:t=3.727,P＜0.05;난치복발조TLR4:t=4.043,P＜0.05;MDSC:t=4.125,P＜0.05;완전완해조TLR4:t=2.798,P＜O.05;MDSC:t=3.469,P ＜0.05).Pearson등급상관분석현시TLR4여MDSC표체정정상관(r=0.673,P＜0.01).결론 TLR4화MDSC대우AML적발생、발전、료효급예후밀절상관,차량자재AML적발생、발전과정중발휘중요협동작용.
Objective To investigate the expression of Toll-like receptor 4 (TLR4) and myeloid derived suppressor cells(MDSC) in bone marrow cells in children with acute myeloid leukemia (AML),and to detect its relationship with the clinical features,the effect of chemotherapy and prognosis.Methods Twenty-nine cases of children with AML were collected from June 2013 to March 2014 in People's Hospital of Wuhan University,in which 11 cases of low-risk group,10 cases of middle-risk group,8 cases of high-risk group;and 17 cases of non blood disease was as the control group.The expressions of TLR4 and MDSC were detected by using reverse transcription-polymerase chain reaction (RT-PCR),Western blot methods,immunohistochemical staining,and flow cytometry,respectively,in the bone marrow cells of 29 children with AML.Results The mRNA and protein expression of TLR4 in the initial treatment group was higher than those in the complete remission group(t =3.092,3.393,all P ＜ 0.05).The mRNA and protein expression of TLR4 in the relapse group was higher than those in the complete remission group(t =4.013,4.279,all P ＜ 0.05).The positive expression rates of MDSC in the above 3 groups were (29.77 ± 1.39) ％,(5.19 ± 0.65) ％,(38.62 ± 3.54) ％,respectively,compared with the control group [(1.32 ± 0.27) ％] and there was significant difference(all P ＜0.05).The positive expression rates of TLR4 and MDSC in the initial treatment group,relapse group and complete remission group were significantly higher than those in the control group,with significant differences (initial treatment group TLR4:t =3.559,P ＜ 0.05;MDSC:t =3.727,P ＜ 0.05;relapse group TLR4:t =4.043,P ＜ 0.05;MDSC:t =4.125,P ＜ 0.05;complete remission group TLR4:t =2.798,P ＜ 0.05;MDSC:t =3.469,P ＜ 0.05).Pearson rank correlation analysis showed that there was a positive correlation between the expression of TLR4 and MDSC (r =0.673,P ＜0.01).Conclusions The expressions of both TLR4 and MDSC play an important role in onset,progression,curative effect and prognosis in children with AML,and the two may play an importment role in synergistic effect.
