CAJ | 학술논문

目的：研究精液白细胞异常增加对精液活性氧(ROS)及精子DNA损伤的影响。方法：共收集52例精液白细胞异常增高标本和50例白细胞正常标本，以鲁米诺为探针，运用化学发光法检测精子活性氧水平。采用精子染色质结构分析(SCSA)和单细胞凝胶电泳(SCGE)分析精子核DNA损伤。结果：白细胞异常组较白细胞正常组精液标本中ROS显著增加(P<0.01)，SCSA结果显示白细胞异常组DNA碎片指数(DFI)、高DNA可染性(HDS)较白细胞正常组显著增加(P=0.020，P=0.012)；SCGE结果显示白细胞异常组彗星尾矩(TM)较白细胞正常组显著增加(P=0.007)。结论：精液白细胞异常升高可引起ROS增加，造成精子核DNA的断裂损伤。
목적：연구정액백세포이상증가대정액활성양(ROS)급정자DNA손상적영향。방법：공수집52례정액백세포이상증고표본화50례백세포정상표본，이로미낙위탐침，운용화학발광법검측정자활성양수평。채용정자염색질결구분석(SCSA)화단세포응효전영(SCGE)분석정자핵DNA손상。결과：백세포이상조교백세포정상조정액표본중ROS현저증가(P<0.01)，SCSA결과현시백세포이상조DNA쇄편지수(DFI)、고DNA가염성(HDS)교백세포정상조현저증가(P=0.020，P=0.012)；SCGE결과현시백세포이상조혜성미구(TM)교백세포정상조현저증가(P=0.007)。결론：정액백세포이상승고가인기ROS증가，조성정자핵DNA적단렬손상。
OBJECTIVE:To study the impacts of leukocyte elevation on reactive oxygen species (ROS) and sperm DNA damage.METHODS:A total of 52 elevated leukocyte specimen and 50 normal leukocytes specimen were collected. ROS production was measured using luminol as the probe by the Chemiluminescence method. Sperm nuclear DNA damage was assessed by sperm chromatin structure assay (SCSA) and single-cell gel electrophoresis (SCGE). RESULTS:ROS levels (using the log-transformation) was significantly higher in the elevated leukocyte group (P<0.01). Data of SCSA indicated there were significant increases ofDNA fragmentation index and high DNA stainable in the elevated leukocytes group (P=0.020,P=0.012). SCGE analysis,in terms of tail moments,was significantly higher in the elevated leukocyte group (P=0.007).CONCLUSION:Leukocyte elevation could result in the increase of reactive oxygen species,which may induce sperm nuclear DNA damage.