癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
Carcinogenesis,Teratogenesis & Mutagenesis
2015年
5期
370-374
,共5页
滕倩%马婷婷%孙永叶%张华琦%马爱国
滕倩%馬婷婷%孫永葉%張華琦%馬愛國
등천%마정정%손영협%장화기%마애국
叶黄素%素没食子儿茶素没食子酸酯%抗氧化%DNA损伤
葉黃素%素沒食子兒茶素沒食子痠酯%抗氧化%DNA損傷
협황소%소몰식자인다소몰식자산지%항양화%DNA손상
lutein%epigallocatechin gallate%antioxidation%DNA damage
目的：探讨补充叶黄素与表没食子儿茶素没食子酸酯(EGCG)对机体抗氧化活性及DNA氧化损伤的影响。方法：选择40名健康青年志愿者，随机分为4组，分别为叶黄素组(20 mg/d)、EGCG组(270 mg/d)、叶黄素(20 mg/d)+EGCG(270 mg/d)组和正常对照组。3个实验组志愿者连续补充叶黄素和/或EGCG 20 d，正常对照组未给任何补充。试验前及试验第21天分别留取受试者清晨空腹静脉血5 mL，分离血浆并收集淋巴细胞。高效液相色谱法检测血浆中叶黄素和EGCG浓度，测定总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量，采用碱性单细胞凝胶电泳法测定外周血淋巴细胞DNA损伤状况。结果：经过20 d后，叶黄素组和叶黄素+EGCG组受试者血浆中叶黄素水平较干预前分别升高了257%和175%(P<0.01)。与正常对照组相比，干预后叶黄素组受试者血浆中MDA含量、T-SOD活力明显下降、GSH-Px活力显著升高(P均<0.05)，EGCG组受试者血浆中MDA含量、T-SOD、GSH-Px活力无明显差异(P均>0.05)，叶黄素和EGCG对机体抗氧化活力无交互作用。DNA损伤分析显示，叶黄素组、EGCG组中由低、中浓度H2O2诱导的外周血淋巴细胞DNA损伤均显著低于对照组(P<0.05)，而由高浓度 H2O2诱导的DNA氧化损伤及自发损伤与对照组比较差异无统计学意义，叶黄素和EGCG对DNA损伤的影响无交互作用(P均>0.05)。结论：叶黄素、EGCG单独补充均可降低一定浓度H2O2诱导的DNA氧化损伤，叶黄素单独补充也可增强机体抗氧化活性；叶黄素与EGCG对机体抗氧化活性及DNA氧化损伤的影响均无交互作用。
目的：探討補充葉黃素與錶沒食子兒茶素沒食子痠酯(EGCG)對機體抗氧化活性及DNA氧化損傷的影響。方法：選擇40名健康青年誌願者，隨機分為4組，分彆為葉黃素組(20 mg/d)、EGCG組(270 mg/d)、葉黃素(20 mg/d)+EGCG(270 mg/d)組和正常對照組。3箇實驗組誌願者連續補充葉黃素和/或EGCG 20 d，正常對照組未給任何補充。試驗前及試驗第21天分彆留取受試者清晨空腹靜脈血5 mL，分離血漿併收集淋巴細胞。高效液相色譜法檢測血漿中葉黃素和EGCG濃度，測定總超氧化物歧化酶(T-SOD)、穀胱甘肽過氧化物酶(GSH-Px)活性和丙二醛(MDA)含量，採用堿性單細胞凝膠電泳法測定外週血淋巴細胞DNA損傷狀況。結果：經過20 d後，葉黃素組和葉黃素+EGCG組受試者血漿中葉黃素水平較榦預前分彆升高瞭257%和175%(P<0.01)。與正常對照組相比，榦預後葉黃素組受試者血漿中MDA含量、T-SOD活力明顯下降、GSH-Px活力顯著升高(P均<0.05)，EGCG組受試者血漿中MDA含量、T-SOD、GSH-Px活力無明顯差異(P均>0.05)，葉黃素和EGCG對機體抗氧化活力無交互作用。DNA損傷分析顯示，葉黃素組、EGCG組中由低、中濃度H2O2誘導的外週血淋巴細胞DNA損傷均顯著低于對照組(P<0.05)，而由高濃度 H2O2誘導的DNA氧化損傷及自髮損傷與對照組比較差異無統計學意義，葉黃素和EGCG對DNA損傷的影響無交互作用(P均>0.05)。結論：葉黃素、EGCG單獨補充均可降低一定濃度H2O2誘導的DNA氧化損傷，葉黃素單獨補充也可增彊機體抗氧化活性；葉黃素與EGCG對機體抗氧化活性及DNA氧化損傷的影響均無交互作用。
목적：탐토보충협황소여표몰식자인다소몰식자산지(EGCG)대궤체항양화활성급DNA양화손상적영향。방법：선택40명건강청년지원자，수궤분위4조，분별위협황소조(20 mg/d)、EGCG조(270 mg/d)、협황소(20 mg/d)+EGCG(270 mg/d)조화정상대조조。3개실험조지원자련속보충협황소화/혹EGCG 20 d，정상대조조미급임하보충。시험전급시험제21천분별류취수시자청신공복정맥혈5 mL，분리혈장병수집림파세포。고효액상색보법검측혈장중협황소화EGCG농도，측정총초양화물기화매(T-SOD)、곡광감태과양화물매(GSH-Px)활성화병이철(MDA)함량，채용감성단세포응효전영법측정외주혈림파세포DNA손상상황。결과：경과20 d후，협황소조화협황소+EGCG조수시자혈장중협황소수평교간예전분별승고료257%화175%(P<0.01)。여정상대조조상비，간예후협황소조수시자혈장중MDA함량、T-SOD활력명현하강、GSH-Px활력현저승고(P균<0.05)，EGCG조수시자혈장중MDA함량、T-SOD、GSH-Px활력무명현차이(P균>0.05)，협황소화EGCG대궤체항양화활력무교호작용。DNA손상분석현시，협황소조、EGCG조중유저、중농도H2O2유도적외주혈림파세포DNA손상균현저저우대조조(P<0.05)，이유고농도 H2O2유도적DNA양화손상급자발손상여대조조비교차이무통계학의의，협황소화EGCG대DNA손상적영향무교호작용(P균>0.05)。결론：협황소、EGCG단독보충균가강저일정농도H2O2유도적DNA양화손상，협황소단독보충야가증강궤체항양화활성；협황소여EGCG대궤체항양화활성급DNA양화손상적영향균무교호작용。
[ABSTRACT]OBJECTIVE:To investigate the effects of lutein and epigallocatechingallate (EGCG) supplementation on the antioxidant activity and DNA damage.METHODS:A total of 40 healthy young adults were randomly divided into four groups (n=10 in each group),including lutein group (20 mg/d),EGCG group (270 mg/d),lutein (20 mg/d)+EGCG (270 mg/d) group and normal control group. Three experimental groups of volunteers were treatedwithlutein andor EGCG for 20 d while the control group wasn’t given any supplement. 5 mL fasting blood was collected at the beginning and the end of the trial. Plasma lutein and EGCG concentrations were measured by HPLC detector systems. Plasma total superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px) and malondial dehyde (MDA) levels were measured by kits. Peripheral blood lymphocyte DNA damage was analyzed by single-cell gel electrophoresis (SCGE).RESULTS:After supplementation for 20 days,plasma lutein concentration of the subjects in the lutein group and lutein+EGCG group increased by 257% and 175%(P<0.01). Plasma MDA and T-SOD concentrations of the subjects in the lutein group were significantly lower than those of the control group,while plasma GSH-Px concentration was significantly increased (P<0.05). No significant difference was found in the antioxidant activity of the subjects in the EGCG group. The combined supplementation of lutein and EGCG had no interaction on the antioxidant activity. DNA damage analysis showed that low-and medium- concentration H2O2-induced DNA damage were markedly lower in either lutein or EGCG group than those in the control group (P<0.05). However high- concentration H2O2-induced and spontaneous DNA damage in both groups did not show any significant difference with those in the control group. The combined supplementation of lutein and EGCG had no interaction on the DNA damage (P>0.05). CONCLUSION:Lutein or EGCG supplementation alone could effectively reduce DNA damage. Lutein supplementation enhanced antioxidant activity,but EGCG did not. Lutein and EGCG did not show any interaction on antioxidant activity and DNA damage.
