癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
Carcinogenesis,Teratogenesis & Mutagenesis
2015年
5期
341-346
,共6页
卫美蓉%刘颂%赵真真%王笑峰%罗兵
衛美蓉%劉頌%趙真真%王笑峰%囉兵
위미용%류송%조진진%왕소봉%라병
胃癌%EB病毒%ToI样受体%单核苷酸多态性
胃癌%EB病毒%ToI樣受體%單覈苷痠多態性
위암%EB병독%ToI양수체%단핵감산다태성
gastric carcinoma%Epstein-Barr virus%Toll-like receptor%single nucleotide polymorphism
目的:探讨Toll样受体2(TLR2) r s3804099和rs3804100基因多态性与胃癌和EB病毒相关胃癌(EBVaGC)易感性的关系。方法:选用185例EBV阴性胃癌(EBVnGC)组织、41例EBVaGC组织以及100位健康人群外周血标本作为研究对象,采用PCR结合限制性片段长度多态性(RFLP)技术检测TLR2 r s3804099与rs3804100的基因多态性。结果:TLR2 r s3804099基因型和等位基因频率在胃癌组与健康对照组22间的差异均有统计学意义(χ=5.617,P=0.018;χ=6.467,P=0.011),胃癌组C等位基因频率及C等位基因携带者频率均明显高于健康对22照组(χ=6.467,P=0.011;χ=4.444,P=0.035),且与野生TT型相比,CC基因型可增加胃癌的发病风险(OR=3.554,95%CI=1.179~10.715)。TLR2 rs3804100位点的各基因型频率、C等位基因及C等位基因携带者的频率在胃癌组和健康对照组之间差异无统计学意义(P>0.05)。TLR2 r s3804099与rs3804100位点的各基因型频率、C等位基因频率及C等位基因携带者频率在EBVaGC和EBVnGC两组间的差异均无统计学意义(P>0.05)。结论:TLR2 rs3804099基因多态性可能与胃癌发病风险有关,C等位基因可能为胃癌的危险因子,携带C等位基因可能增加胃癌的发病风险。TLR2 r s3804099与rs3804100基因的多态性与EBVaGC的易感性无明显相关性。
目的:探討Toll樣受體2(TLR2) r s3804099和rs3804100基因多態性與胃癌和EB病毒相關胃癌(EBVaGC)易感性的關繫。方法:選用185例EBV陰性胃癌(EBVnGC)組織、41例EBVaGC組織以及100位健康人群外週血標本作為研究對象,採用PCR結閤限製性片段長度多態性(RFLP)技術檢測TLR2 r s3804099與rs3804100的基因多態性。結果:TLR2 r s3804099基因型和等位基因頻率在胃癌組與健康對照組22間的差異均有統計學意義(χ=5.617,P=0.018;χ=6.467,P=0.011),胃癌組C等位基因頻率及C等位基因攜帶者頻率均明顯高于健康對22照組(χ=6.467,P=0.011;χ=4.444,P=0.035),且與野生TT型相比,CC基因型可增加胃癌的髮病風險(OR=3.554,95%CI=1.179~10.715)。TLR2 rs3804100位點的各基因型頻率、C等位基因及C等位基因攜帶者的頻率在胃癌組和健康對照組之間差異無統計學意義(P>0.05)。TLR2 r s3804099與rs3804100位點的各基因型頻率、C等位基因頻率及C等位基因攜帶者頻率在EBVaGC和EBVnGC兩組間的差異均無統計學意義(P>0.05)。結論:TLR2 rs3804099基因多態性可能與胃癌髮病風險有關,C等位基因可能為胃癌的危險因子,攜帶C等位基因可能增加胃癌的髮病風險。TLR2 r s3804099與rs3804100基因的多態性與EBVaGC的易感性無明顯相關性。
목적:탐토Toll양수체2(TLR2) r s3804099화rs3804100기인다태성여위암화EB병독상관위암(EBVaGC)역감성적관계。방법:선용185례EBV음성위암(EBVnGC)조직、41례EBVaGC조직이급100위건강인군외주혈표본작위연구대상,채용PCR결합한제성편단장도다태성(RFLP)기술검측TLR2 r s3804099여rs3804100적기인다태성。결과:TLR2 r s3804099기인형화등위기인빈솔재위암조여건강대조조22간적차이균유통계학의의(χ=5.617,P=0.018;χ=6.467,P=0.011),위암조C등위기인빈솔급C등위기인휴대자빈솔균명현고우건강대22조조(χ=6.467,P=0.011;χ=4.444,P=0.035),차여야생TT형상비,CC기인형가증가위암적발병풍험(OR=3.554,95%CI=1.179~10.715)。TLR2 rs3804100위점적각기인형빈솔、C등위기인급C등위기인휴대자적빈솔재위암조화건강대조조지간차이무통계학의의(P>0.05)。TLR2 r s3804099여rs3804100위점적각기인형빈솔、C등위기인빈솔급C등위기인휴대자빈솔재EBVaGC화EBVnGC량조간적차이균무통계학의의(P>0.05)。결론:TLR2 rs3804099기인다태성가능여위암발병풍험유관,C등위기인가능위위암적위험인자,휴대C등위기인가능증가위암적발병풍험。TLR2 r s3804099여rs3804100기인적다태성여EBVaGC적역감성무명현상관성。
OBJECTIVE:The aim of our study was to evaluate the potential associations between the single nucleotide polymorphisms (SNPs) of Toll-like receptor (TLR2) rs3804099 and rs3804100 genes and the risk of gastric carcinoma(GC),especially to Epstein-Barr virus-associated gastirc carcinoma (EBVaGC).METHODS:TLR2 rs3804099 and rs3804100 gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 185 cases of EBV-negative GC,41 cases of EBVaGC. 100 cases of peripheral blood samples from healthy individuals were also examined. The data was analysed by SPSS.RESULTS:As for theTLR2 gene (rs3804099), there was significant difference between the GC group and the control group in both genotype and allelic 22frequencies (χ=5.617,P=0.018;χ=6.467,P=0.011). The C allele and C allele carriers frequencies of gastric carcinoma 22group were significantly higher than those of controls (χ=6.467,P=0.011;χ=4.444,P=0.035). Compared with the wild type TT, the CC genotype could increase the risk of gastric cancer (OR=3.554,95%CI=1.179-10.715). As for theTLR2 gene (rs38040100),there were no significant differences between the GC group and the control group in genotype,C allelic frequency and C allele carrier frequency (P>0.05). In all the indicators,no polymorphism was found to be related to EBVaGC in the studied population (P>0.05).CONCLUSION:There was an association betweenTLR2 rs3804099 gene polymorphism and gastric carcinoma,and the C allele may be a risk factor for gastric carcinoma. Carrying the C allele may increase the risk of gastric carcinoma. No association was observed betweenTLR2 (rs3804099 and rs38040100) gene polymorphism and EBVaGC.