CAJ | 학술논문

目的：测定土槿皮、侧柏叶、苦参、丁香、肉桂及其不同组合对糠秕马拉色菌的最小抑菌浓度,观察其体外抑菌效果。方法参照美国国家临床实验室标准化委员会制订的M27-A方案中酵母菌微量稀释法,在此基础上进行改良,采用含油的培养基作为药敏试验的液基,制备微量药敏板,对糠秕马拉色菌进行体外药敏试验。结果最低抑菌浓度( minimum inhibitory concentration, MIC)依次为：5种中药混合液(槿柏洗剂)：5.5 mg/mL,土槿皮+侧柏叶+苦参+丁香：16 mg/mL,土槿皮+侧柏叶+苦参：8.5 mg/mL,丁香+肉桂：9 mg/mL,土槿皮：10 mg/mL。结论相比于各单味药及不同组合,槿柏洗剂全方MIC为最小,5种中药及其不同组合混合液均有抑制糠秕马拉色菌生长的作用。
목적：측정토근피、측백협、고삼、정향、육계급기불동조합대강비마랍색균적최소억균농도,관찰기체외억균효과。방법삼조미국국가림상실험실표준화위원회제정적M27-A방안중효모균미량희석법,재차기출상진행개량,채용함유적배양기작위약민시험적액기,제비미량약민판,대강비마랍색균진행체외약민시험。결과최저억균농도( minimum inhibitory concentration, MIC)의차위：5충중약혼합액(근백세제)：5.5 mg/mL,토근피+측백협+고삼+정향：16 mg/mL,토근피+측백협+고삼：8.5 mg/mL,정향+육계：9 mg/mL,토근피：10 mg/mL。결론상비우각단미약급불동조합,근백세제전방MIC위최소,5충중약급기불동조합혼합액균유억제강비마랍색균생장적작용。
Objective To observe the inhibitive effect of the cortex pseudolaricis, oriental arborvitae, sophora flavescens ait, clove, cinnamon and their different combination on Malassezia furfur in vitro. Methods Based on the M27-A scheme about yeast micro dilution method, which was developed by the national clinical laboratory standardization committee (NCCLS),we prepared drug sensitive plate and liquid medium with oil to test the sensitivity of Chinese herb to Malassezia furfur. Results Minimum inhibitory concentration(MIC):5 kinds of Chinese herbal mixture (the Jin Bai lotion):5. 5mg/mL,cortex pseudolaricis+oriental arborvitae+sophora flavescens ait+clove:16mg/mL, cortex pseudolaricis+oriental arborvitae+sophora flavescens ait:8. 5mg/mL, clove+cinnamon:9mg/mL, cortex pseudolaricis:10mg/mL. Conclusions Compared to the single herb and the different combinations, the MIC of Jin Bai lotion was the smallest. Five kinds of Chinese herbal and their different mixture can inhibit the Malassezia furfur.