环球中医药
環毬中醫藥
배구중의약
Global Traditional Chinese Medicine
2015年
10期
1158-1163
,共6页
李秋萍%蒋俏丽%翟文文%戴俊东
李鞦萍%蔣俏麗%翟文文%戴俊東
리추평%장초려%적문문%대준동
姜黄素%胡椒碱%复方自微乳药物制剂%人工结肠液%小鼠结肠组织匀浆液%稳定性
薑黃素%鬍椒堿%複方自微乳藥物製劑%人工結腸液%小鼠結腸組織勻漿液%穩定性
강황소%호초감%복방자미유약물제제%인공결장액%소서결장조직균장액%은정성
Curcumin%Piperine%Compound self-microemulsifying pharmaceutical formulations%Artificial colon fluid%The mice colonic tissue homogenized mixture%Stability
目的：考察姜黄素—胡椒碱复方自微乳给药系统对姜黄素在结肠部位稳定性的改善作用,为其治疗结肠炎或结肠癌等结肠局部疾病提供科学依据。方法通过HPLC测定姜黄素的含量,37℃体外考察并比较姜黄素溶液、姜黄素—胡椒碱混合溶液及姜黄素—胡椒碱复方自微乳在人工结肠液及小鼠结肠组织匀浆液中的稳定性。结果37℃人工结肠液中孵育24小时后,姜黄素溶液、姜黄素—胡椒碱混合溶液和姜黄素—胡椒碱复方自微乳中姜黄素的降解百分率分别为19．93%、12．67%和10．50%,降解过程符合一级动力学方程,半衰期分别为19．67小时、57．87小时和75．23小时；37℃小鼠结肠组织匀浆液中孵育8小时后,姜黄素溶液、姜黄素—胡椒碱混合溶液和姜黄素-胡椒碱复方自微乳中姜黄素的降解百分率分别为19．96%、14．49%和4．63%。结论姜黄素-胡椒碱复方自微乳给药系统可以显著改善姜黄素在结肠部位的的稳定性(P<0．05),有望增强对结肠局部疾病的治疗效果。
目的：攷察薑黃素—鬍椒堿複方自微乳給藥繫統對薑黃素在結腸部位穩定性的改善作用,為其治療結腸炎或結腸癌等結腸跼部疾病提供科學依據。方法通過HPLC測定薑黃素的含量,37℃體外攷察併比較薑黃素溶液、薑黃素—鬍椒堿混閤溶液及薑黃素—鬍椒堿複方自微乳在人工結腸液及小鼠結腸組織勻漿液中的穩定性。結果37℃人工結腸液中孵育24小時後,薑黃素溶液、薑黃素—鬍椒堿混閤溶液和薑黃素—鬍椒堿複方自微乳中薑黃素的降解百分率分彆為19．93%、12．67%和10．50%,降解過程符閤一級動力學方程,半衰期分彆為19．67小時、57．87小時和75．23小時；37℃小鼠結腸組織勻漿液中孵育8小時後,薑黃素溶液、薑黃素—鬍椒堿混閤溶液和薑黃素-鬍椒堿複方自微乳中薑黃素的降解百分率分彆為19．96%、14．49%和4．63%。結論薑黃素-鬍椒堿複方自微乳給藥繫統可以顯著改善薑黃素在結腸部位的的穩定性(P<0．05),有望增彊對結腸跼部疾病的治療效果。
목적：고찰강황소—호초감복방자미유급약계통대강황소재결장부위은정성적개선작용,위기치료결장염혹결장암등결장국부질병제공과학의거。방법통과HPLC측정강황소적함량,37℃체외고찰병비교강황소용액、강황소—호초감혼합용액급강황소—호초감복방자미유재인공결장액급소서결장조직균장액중적은정성。결과37℃인공결장액중부육24소시후,강황소용액、강황소—호초감혼합용액화강황소—호초감복방자미유중강황소적강해백분솔분별위19．93%、12．67%화10．50%,강해과정부합일급동역학방정,반쇠기분별위19．67소시、57．87소시화75．23소시；37℃소서결장조직균장액중부육8소시후,강황소용액、강황소—호초감혼합용액화강황소-호초감복방자미유중강황소적강해백분솔분별위19．96%、14．49%화4．63%。결론강황소-호초감복방자미유급약계통가이현저개선강황소재결장부위적적은정성(P<0．05),유망증강대결장국부질병적치료효과。
Objective To investigate whether the curcumin-piperine dual drug loaded self-microe-mulsifying drug delivery system ( Cur-PIP-SMEDDS ) could enhance the curcumin’s stability in the colon site. This study could provide scientific basis for the treatment of colonic disease, such as colitis and colon cancer. Methods HPLC method was established for the determination of the content of curcumin. To determine whether Cur-PIP-SMEDDS is more stable in the colonic site, free curcumin, curcumin-piperine mixture and Cur-PIP-SMEDDS were incubated in the artificial colon fluid and the mice colonic tissue homogenized mixture at 37℃, and sampled periodically to determine the concentration of curcumin by HPLC. Results The degradation percentage of curcumin in free curcumin solution, curcumin-piperine mixture, and Cur-PIP-SMEDDS were 19. 93%, 12. 67% and 10. 50% respectively after incubation for 24 hours in the artificial colon fluid at 37℃. The degradation process of curcumin followed apparent first-order kinetics equation, and the half-life were 19. 67 h, 57. 87 h and 75. 23 h respectively. The degradation percentage of curcumin in free curcumin solution, curcumin-piperine mixture, and Cur-PIP-SMEDDS were 19. 96%, 14. 49% and 4. 63% respectively. After incubation for 8 hours in the mice colonic tissue homogenized mixture at 37℃. Conclusion The results showed that Cur-PIP-SMEDDS could significantly enhance the stability of curcumin ( P<0. 05 ) in the colonic site, and may enhance the efficacy for the treatment of colonic disease.
