CAJ | 학술논문

目的：探讨维生素E琥珀酸酯(VES)对人食管鳞癌低分化细胞株TE-1以及高分化细胞株Eca-109增殖的抑制作用及诱发凋亡的作用。方法：不同浓度(0、5、10、15、20μg/mL)VES分别处理TE-1及Eca-109细胞24 h后，采用MTT法检测细胞增殖情况；光镜下观察细胞形态；DAPI染色观察细胞核凋亡形态，Western blot方法检测细胞中凋亡标志蛋白多聚ADP核糖聚合酶(PARP)的表达情况。结果：与对照组比较， MTT检测结果显示随着VES剂量增加，两种细胞株均出现了不同程度的增殖抑制，在20μg/mL VES组细胞增殖率均达到最低(TE-1：15.89%±6.22%；Eca-109：13.78%±4.89%)。光镜下细胞形态随着VES剂量增加发生明显改变，TE-1细胞在10μg/mL VES组开始出现细胞变圆、体积缩小、漂浮死亡等形态；Eca-109细胞在15μg/mL VES组开始出现细胞变圆、体积缩小、漂浮死亡等形态。DAPI染色结果显示， VES高剂量组(20μg/mL)两株细胞的细胞核均出现明显的凋亡形态。Western blot显示，随着VES剂量增加，凋亡标记蛋白PARP裂解激活，在20μg/mL VES处理组达到最大裂解程度。结论：VES对低分化及高分化人食管癌细胞的增殖均有抑制作用并诱导细胞发生凋亡。
목적：탐토유생소E호박산지(VES)대인식관린암저분화세포주TE-1이급고분화세포주Eca-109증식적억제작용급유발조망적작용。방법：불동농도(0、5、10、15、20μg/mL)VES분별처리TE-1급Eca-109세포24 h후，채용MTT법검측세포증식정황；광경하관찰세포형태；DAPI염색관찰세포핵조망형태，Western blot방법검측세포중조망표지단백다취ADP핵당취합매(PARP)적표체정황。결과：여대조조비교， MTT검측결과현시수착VES제량증가，량충세포주균출현료불동정도적증식억제，재20μg/mL VES조세포증식솔균체도최저(TE-1：15.89%±6.22%；Eca-109：13.78%±4.89%)。광경하세포형태수착VES제량증가발생명현개변，TE-1세포재10μg/mL VES조개시출현세포변원、체적축소、표부사망등형태；Eca-109세포재15μg/mL VES조개시출현세포변원、체적축소、표부사망등형태。DAPI염색결과현시， VES고제량조(20μg/mL)량주세포적세포핵균출현명현적조망형태。Western blot현시，수착VES제량증가，조망표기단백PARP렬해격활，재20μg/mL VES처리조체도최대렬해정도。결론：VES대저분화급고분화인식관암세포적증식균유억제작용병유도세포발생조망。
OBJECTIVE:To study the effects of vitamin E succinate(VES) on proliferation,apoptosis and expressions of associated protein of human esophageal carcinoma cell line Eca-109 and TE-1.METHODS:Eca-109 and TE-1 human esophageal carcinoma cells were culturedin vitro. Cells were treated with different concentrations of VES for 24 h. MTT was used to measure the proliferation of cells. Morphological changes of cells were examined by inverted microscopy. Apoptosis was assessed by DAPI staining. The expression of poly ADP-ribose polymerase(PARP) of two cell lines among different concentration groups was examined by Western blotting.RESULTS:The inhibitory rate increased gradually with increasing concentration of vitamin E succinate (P<0.05). The number of dead cells decreased with increasing concentration of VES. Apoptosis appeared in different degrees in cellswith increasing concentration of VES. The cleavage of PARPprotein in two cell lines also increased gradually.CONCLUSION:Vitamin E succinate could inhibit proliferation and induce apoptosis of Eca-109 and TE-1 cell lines.