国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
International Journal of Respiration
2015年
16期
1226-1229
,共4页
苏强%徐强松%马妮娜%李琴%俞静%张晨光
囌彊%徐彊鬆%馬妮娜%李琴%俞靜%張晨光
소강%서강송%마니나%리금%유정%장신광
FK866%顺铂%非小细胞肺癌%凋亡
FK866%順鉑%非小細胞肺癌%凋亡
FK866%순박%비소세포폐암%조망
FK866%Cisplatin%Non-small cell lung cancer%Apoptosis
目的 探讨FK866联合顺铂对非小细胞肺癌A549细胞凋亡的作用及其可能的机制.方法 FK866单药、顺铂单药和两者联合用药分别作用于非小细胞肺癌A549细胞72 h后,MTT法检测细胞活力,用流式细胞学检测细胞凋亡率.结果 FK866联合顺铂组相对于单药组更能降低非小细胞肺癌A549的细胞活力,流式细胞学检测显示联合用药组比单药组更能促进A549细胞的凋亡.结论 初步得出FK866联合顺铂组比顺铂单药组更能抑制非小细胞肺癌A549细胞活力,促进A549细胞的凋亡.
目的 探討FK866聯閤順鉑對非小細胞肺癌A549細胞凋亡的作用及其可能的機製.方法 FK866單藥、順鉑單藥和兩者聯閤用藥分彆作用于非小細胞肺癌A549細胞72 h後,MTT法檢測細胞活力,用流式細胞學檢測細胞凋亡率.結果 FK866聯閤順鉑組相對于單藥組更能降低非小細胞肺癌A549的細胞活力,流式細胞學檢測顯示聯閤用藥組比單藥組更能促進A549細胞的凋亡.結論 初步得齣FK866聯閤順鉑組比順鉑單藥組更能抑製非小細胞肺癌A549細胞活力,促進A549細胞的凋亡.
목적 탐토FK866연합순박대비소세포폐암A549세포조망적작용급기가능적궤제.방법 FK866단약、순박단약화량자연합용약분별작용우비소세포폐암A549세포72 h후,MTT법검측세포활력,용류식세포학검측세포조망솔.결과 FK866연합순박조상대우단약조경능강저비소세포폐암A549적세포활력,류식세포학검측현시연합용약조비단약조경능촉진A549세포적조망.결론 초보득출FK866연합순박조비순박단약조경능억제비소세포폐암A549세포활력,촉진A549세포적조망.
Objective To investigate if FK866,the inhibitor of nicotinamide phosphoribosyltransferase (NAMPT),was able to enhance the inhibitory effect of cisplatin on non-small cell lung cancer A549 cells.Methods A549 cells were treated with 4 mg/L cisplatin combined with different concentrations of FK866 for 72 hours.MTT assays was then used to detect cell viability and flow cytometry was used to examine apoptosis rate of A549 cells,respectively.Results The combined treatment significantly reduced the activity of A549 cells,and induced higher apoptosis rate in A549 cells than single FK866 or cisplatin.Conclusions FK866 could enhance cisplatin-induced apoptosis in non-small cell lung cancer A549 cells.
