CAJ | 학술논문

目的：探讨不明原因复发性自然流产（URSA）患者蜕膜组织中叉头样转录因子 P3（FOXP3）基因的蛋白表达水平及其与启动子甲基化水平的关系。方法利用蛋白印迹法检测20例URSA 患者（URSA 组）和20名正常妊娠主动要求人工流产术的健康妇女（正常对照组）蜕膜组织中FOXP3蛋白表达情况，利用结合重亚硫酸盐的测序法（BSP）检测2组 FOXP3基因启动子甲基化情况，并分析两者的相关性。结果URSA 组蜕膜组织中 FOXP3表达水平明显低于正常对照组（P ＜0.01）。BSP 显示 URSA 组 FOXP3基因启动子甲基化水平明显高于正常对照组（P ＜0.01）。FOXP3蛋白表达水平与 FOXP3基因启动子甲基化水平呈负相关（r ＝－0.917，P ＜0.01）。结论FOXP3基因启动子甲基化水平升高后，FOXP3基因蛋白表达水平下调，引起免疫耐受异常，可能导致 RSA 的发病。
목적：탐토불명원인복발성자연유산（URSA）환자세막조직중차두양전록인자 P3（FOXP3）기인적단백표체수평급기여계동자갑기화수평적관계。방법이용단백인적법검측20례URSA 환자（URSA 조）화20명정상임신주동요구인공유산술적건강부녀（정상대조조）세막조직중FOXP3단백표체정황，이용결합중아류산염적측서법（BSP）검측2조 FOXP3기인계동자갑기화정황，병분석량자적상관성。결과URSA 조세막조직중 FOXP3표체수평명현저우정상대조조（P ＜0.01）。BSP 현시 URSA 조 FOXP3기인계동자갑기화수평명현고우정상대조조（P ＜0.01）。FOXP3단백표체수평여 FOXP3기인계동자갑기화수평정부상관（r ＝－0.917，P ＜0.01）。결론FOXP3기인계동자갑기화수평승고후，FOXP3기인단백표체수평하조，인기면역내수이상，가능도치 RSA 적발병。
Objective To investigate the association between the level of FOXP3 promoter methyla-tion and the expression level of FOXP3 protein in the decidua of patients with unexplained recurrent spontane-ous abortion (URSA).Methods The expression levels of FOXP3 protein in the decidua of 20 URSA patients and 20 healthy counterparts who underwent induced abortion were assessed by Western blot.Bisulfite-assisted genomic sequencing PCR (BSP)was utilized to detect the levels of FOXP3 promoter methylation.The correla-tion between two indexes was evaluated.Results The expression level of FOXP3 in the decidua of the URSA group was significantly lower than that of the control group (P <0.01),whereas the level of FOXP3 promoter methylation in the URSA group was significantly higher compared with that in the control group (P <0.01).The expression level of FOXP3 protein was negatively correlated with the level of FOXP3 promoter methylation (r =-0.917,P <0.01).Conclusion As the level of FOXP3 promoter methylation increases,the expres-sion level of FOXP3 protein is down-regulated,which may cause abnormal immune tolerance and potentially lead to the incidence of RSA.