CAJ | 학술논문

为了预测和分析拟南芥(Arabidopsis thaliana)转录终止因子PDE191蛋白质的结构与功能,采用生物信息学的方法对PDE191蛋白质进行了系统研究,包括PDE191蛋白质的理化性质、跨膜区和信号肽、亚细胞定位、二级结构、功能域、蛋白质的功能分类预测、多重序列比对与系统发育树构建、三级结构建模。结果表明,拟南芥PDE191蛋白质属于植物mTERF蛋白质家族的成员,其蛋白质相对分子质量为37.89 ku,等电点为9.12,不具有信号肽和跨膜区。该蛋白质定位于细胞叶绿体,N端1-30位氨基酸为前导肽序列。其二级结构主要为a螺旋和无规则卷曲,包含7个mTERF基序,三级结构显示结果与二级结构预测结果相符。蛋白质多重序列比对和聚类分析显示,在玉米、蓖麻、杨树、大豆、葡萄、水稻和高粱等高等植物中存在与拟南芥PDE191蛋白质高度同源性的蛋白质,尤其是与玉米PDE191蛋白质相似性高达99％。
위료예측화분석의남개(Arabidopsis thaliana)전록종지인자PDE191단백질적결구여공능,채용생물신식학적방법대PDE191단백질진행료계통연구,포괄PDE191단백질적이화성질、과막구화신호태、아세포정위、이급결구、공능역、단백질적공능분류예측、다중서렬비대여계통발육수구건、삼급결구건모。결과표명,의남개PDE191단백질속우식물mTERF단백질가족적성원,기단백질상대분자질량위37.89 ku,등전점위9.12,불구유신호태화과막구。해단백질정위우세포협록체,N단1-30위안기산위전도태서렬。기이급결구주요위a라선화무규칙권곡,포함7개mTERF기서,삼급결구현시결과여이급결구예측결과상부。단백질다중서렬비대화취류분석현시,재옥미、비마、양수、대두、포도、수도화고량등고등식물중존재여의남개PDE191단백질고도동원성적단백질,우기시여옥미PDE191단백질상사성고체99％。
To research the structure and biological function of the A rabidopsis thaliana transcription termination factor PDE191, bioinformatic methods were used to make systematical analysis, including physical and chemical properties, hy-drophobicity, transmembrane region, signal peptide, secondary structure, functional domain and assortment, multiple align-ment, phylogenetic tree and three-dimensional structures. The results showed that PDE191 belonged to hydrophilic protein, and the calculated molecular mass was 37.89 ku and the theoretical isoelectric point was 9.12, without signal peptide and transmembrane regions; PDE191 localized in chloroplast with a transit peptide of 1-30 aa at the N-terminal; the secondary structure was mainly composed of a-helix and random coil, and contained seven mTERF motifs; the three-demensional structure prediction expressed similar result with secondary structure prediction; the multiple alignment and clustering analysis showed that PDE191 was a orthologous protein of mTERF protein family, and had high similarity to those in zea mays, rici-nus communis,glycine max,vitis vinifera,populus trichocarpa,sorghum bicolor,and oryza sativa proteins. Our research provided bioinformation for the further study of the function of Arabidopsis thaliana PDE191.