中国医学创新
中國醫學創新
중국의학창신
Medical Innovation of China
2015年
27期
16-20
,共5页
FK1706%营养神经%自体神经移植%神经再生
FK1706%營養神經%自體神經移植%神經再生
FK1706%영양신경%자체신경이식%신경재생
FK1706%Neurotrophy%Nerve autograft%Neuroregeneration
目的：探讨FK1706是否能够促进外周神经移植术后大鼠神经轴突再生。方法：20只雄性SD大鼠，行左侧坐骨神经离断、自体桡神经移植术。术后随机分为两组，A组术后当天开始患肢局部肌肉注射FK1706（0.32 mg/kg），持续8周。B组作为对照组不施加干预，仅常规喂养。术后8周，行大鼠左坐骨神经再生有髓神经纤维数及截面积测定、神经电生理检测、左腓肠肌肌湿重测定。结果：A组近端有髓神经纤维数与B组比较差异无统计学意义（P>0.05），但远端有髓神经纤维数、远端纤维截面积明显优于B组，差异有统计学意义（P<0.01）。A组再生有髓神经纤维的髓鞘厚度（mt）及直径比（d/D）明显优于B组，差异有统计学意义（P<0.05）。A组复合运动动作电位（CMAP）、运动神经传导速度（MNCV）及腓肠肌肌湿重明显优于B组，差异有统计学意义（P<0.01）。结论：大鼠坐骨神经移植术后应用FK1706具有神经营养作用，可加快神经功能的恢复。
目的：探討FK1706是否能夠促進外週神經移植術後大鼠神經軸突再生。方法：20隻雄性SD大鼠，行左側坐骨神經離斷、自體橈神經移植術。術後隨機分為兩組，A組術後噹天開始患肢跼部肌肉註射FK1706（0.32 mg/kg），持續8週。B組作為對照組不施加榦預，僅常規餵養。術後8週，行大鼠左坐骨神經再生有髓神經纖維數及截麵積測定、神經電生理檢測、左腓腸肌肌濕重測定。結果：A組近耑有髓神經纖維數與B組比較差異無統計學意義（P>0.05），但遠耑有髓神經纖維數、遠耑纖維截麵積明顯優于B組，差異有統計學意義（P<0.01）。A組再生有髓神經纖維的髓鞘厚度（mt）及直徑比（d/D）明顯優于B組，差異有統計學意義（P<0.05）。A組複閤運動動作電位（CMAP）、運動神經傳導速度（MNCV）及腓腸肌肌濕重明顯優于B組，差異有統計學意義（P<0.01）。結論：大鼠坐骨神經移植術後應用FK1706具有神經營養作用，可加快神經功能的恢複。
목적：탐토FK1706시부능구촉진외주신경이식술후대서신경축돌재생。방법：20지웅성SD대서，행좌측좌골신경리단、자체뇨신경이식술。술후수궤분위량조，A조술후당천개시환지국부기육주사FK1706（0.32 mg/kg），지속8주。B조작위대조조불시가간예，부상규위양。술후8주，행대서좌좌골신경재생유수신경섬유수급절면적측정、신경전생리검측、좌비장기기습중측정。결과：A조근단유수신경섬유수여B조비교차이무통계학의의（P>0.05），단원단유수신경섬유수、원단섬유절면적명현우우B조，차이유통계학의의（P<0.01）。A조재생유수신경섬유적수초후도（mt）급직경비（d/D）명현우우B조，차이유통계학의의（P<0.05）。A조복합운동동작전위（CMAP）、운동신경전도속도（MNCV）급비장기기습중명현우우B조，차이유통계학의의（P<0.01）。결론：대서좌골신경이식술후응용FK1706구유신경영양작용，가가쾌신경공능적회복。
Objective:To investigate whether FK1706 can promote the axon regeneration of peripheral nerve for rats after neural transplantation.Method:20 male SD rats were randomly assigned into two groups after they were performed with left sciatic nerve transection and autologous radial nerve grafting.Group A received FK1706 (0.32 mg/kg) local intramuscular injected once a day on left lower limb for 8 weeks, while Group B as control were routinely fed without special intervention. Electrophysiological studies of left sciatic nerve were performed.The number and area of the regenerated myelinated nerve fiber in cross section pane, and wet muscle weight of the left gastrocnemius muscle were measured,respectively,at 8 weeks after operation.Result:The number of myelinated nerve fiber in proximal end, two groups has no significant difference in statistic (P>0.05), however, in distal end,number of myelinated nerve fiber in Group A was significantly higher than in Group B(P<0.01). The thickness of myelin sheath (mt) of the myelinated nerve fiber, and the ratio that bare axon diameter divided by the total fiber diameter (d/D) were more favorable in Group A than the other, which difference has statistical significance(P<0.05).The wet muscle weight of left gastrocnemius muscle, as well as the compound motor action potential (CMAP) amplitude and motor nerve conduction velocity (MNCV) in Group A,were much better than in Group B,with significant differences (P<0.01).Conclusion:FK1706 has neurotrophy effect that, significantly promote neuroregeneration in rats after autologous nerve transplantation repairing surgery.
