中南医学科学杂志
中南醫學科學雜誌
중남의학과학잡지
Medical Science Journal of Central South China
2015年
5期
515-519
,共5页
符晖%王桥生%刘雁%周斌%彭忠田%梁咸章
符暉%王橋生%劉雁%週斌%彭忠田%樑鹹章
부휘%왕교생%류안%주빈%팽충전%량함장
辛伐他汀%神经源性肺水肿%肺微血管内皮细胞%Bax%Bcl-2
辛伐他汀%神經源性肺水腫%肺微血管內皮細胞%Bax%Bcl-2
신벌타정%신경원성폐수종%폐미혈관내피세포%Bax%Bcl-2
simvastatin%neurogenic pulmonary edema%pulmonary microvascular endothelial cells%Bax%Bcl-2
目的：观察辛伐他汀对神经源性肺水肿( NPE)大鼠血清孵育后肺微血管内皮细胞( PMECs)生长的影响及Bax和Bcl-2表达的影响。方法制备NPE大鼠模型的血清,将体外培养的大鼠PMECs随机分成正常对照组、NPE24 h组、NPE48 h组、辛伐他汀24 h组、辛伐他汀48 h组；NPE组予NPE大鼠模型的血清分别孵育24 h和48 h,辛伐他汀组在NPE大鼠血清孵育的基础上予1μmol/L辛伐他汀干预24 h和48 h。通过细胞计数法描记生长曲线观察各组细胞生长情况,Western-bolt和RT-PCR分别检测各组PMECs内Bax和Bcl-2蛋白与mRNA表达情况。结果 NPE24 h组及48 h组PMECs生长明显减慢,倍增时间延长,有大量漂浮的细胞碎片；辛伐他汀24 h组和48 h组PMECs数量显著增高。同正常对照组相比,NPE24 h组和48 h组PMECs内Bax蛋白和mRNA表达显著增高(P<0.05),而Bcl-2蛋白和mRNA表达降低(P<0.05)。与相应NPE组相比,辛伐他汀24 h组及48 h组Bax蛋白和mRNA表达显著降低(P<0.05),而Bcl-2蛋白和mRNA表达增高(P<0.05)。同辛伐他汀24 h组相比,辛伐他汀48 h组PMECs内Bcl-2蛋白和mRNA表达显著增高(P<0.05)。结论辛伐他汀可改善NPE大鼠血清孵育后PMECs的生长,其机制可能与上调Bcl-2和下调Bax表达有关。
目的：觀察辛伐他汀對神經源性肺水腫( NPE)大鼠血清孵育後肺微血管內皮細胞( PMECs)生長的影響及Bax和Bcl-2錶達的影響。方法製備NPE大鼠模型的血清,將體外培養的大鼠PMECs隨機分成正常對照組、NPE24 h組、NPE48 h組、辛伐他汀24 h組、辛伐他汀48 h組；NPE組予NPE大鼠模型的血清分彆孵育24 h和48 h,辛伐他汀組在NPE大鼠血清孵育的基礎上予1μmol/L辛伐他汀榦預24 h和48 h。通過細胞計數法描記生長麯線觀察各組細胞生長情況,Western-bolt和RT-PCR分彆檢測各組PMECs內Bax和Bcl-2蛋白與mRNA錶達情況。結果 NPE24 h組及48 h組PMECs生長明顯減慢,倍增時間延長,有大量漂浮的細胞碎片；辛伐他汀24 h組和48 h組PMECs數量顯著增高。同正常對照組相比,NPE24 h組和48 h組PMECs內Bax蛋白和mRNA錶達顯著增高(P<0.05),而Bcl-2蛋白和mRNA錶達降低(P<0.05)。與相應NPE組相比,辛伐他汀24 h組及48 h組Bax蛋白和mRNA錶達顯著降低(P<0.05),而Bcl-2蛋白和mRNA錶達增高(P<0.05)。同辛伐他汀24 h組相比,辛伐他汀48 h組PMECs內Bcl-2蛋白和mRNA錶達顯著增高(P<0.05)。結論辛伐他汀可改善NPE大鼠血清孵育後PMECs的生長,其機製可能與上調Bcl-2和下調Bax錶達有關。
목적：관찰신벌타정대신경원성폐수종( NPE)대서혈청부육후폐미혈관내피세포( PMECs)생장적영향급Bax화Bcl-2표체적영향。방법제비NPE대서모형적혈청,장체외배양적대서PMECs수궤분성정상대조조、NPE24 h조、NPE48 h조、신벌타정24 h조、신벌타정48 h조；NPE조여NPE대서모형적혈청분별부육24 h화48 h,신벌타정조재NPE대서혈청부육적기출상여1μmol/L신벌타정간예24 h화48 h。통과세포계수법묘기생장곡선관찰각조세포생장정황,Western-bolt화RT-PCR분별검측각조PMECs내Bax화Bcl-2단백여mRNA표체정황。결과 NPE24 h조급48 h조PMECs생장명현감만,배증시간연장,유대량표부적세포쇄편；신벌타정24 h조화48 h조PMECs수량현저증고。동정상대조조상비,NPE24 h조화48 h조PMECs내Bax단백화mRNA표체현저증고(P<0.05),이Bcl-2단백화mRNA표체강저(P<0.05)。여상응NPE조상비,신벌타정24 h조급48 h조Bax단백화mRNA표체현저강저(P<0.05),이Bcl-2단백화mRNA표체증고(P<0.05)。동신벌타정24 h조상비,신벌타정48 h조PMECs내Bcl-2단백화mRNA표체현저증고(P<0.05)。결론신벌타정가개선NPE대서혈청부육후PMECs적생장,기궤제가능여상조Bcl-2화하조Bax표체유관。
Objective To observe the effect of simvastatin on the growth of pulmonary microvascular endothelial cells (PMECs) incubated in the serum of neurogenic pulmonary edema (NPE) rat and expression of Bax and Bcl-2 of PMECs. Methods The PMECs were randomly divided into five groups,the control group,NPE 24 h group,NPE 48 h group,simvastatin 24 h group and simvastatin 48 h group. The two NPE groups were incubated in rat serum for 24 h and 48 h respectively. The two simvastatin groups were treated with 1 μmol/L simvastatin,also incubated in rat serum for 24 h and 48 h respectively. The cell growth in each group was determined by the cell growth curve of cytometry,while the expression of Bax and Bcl-2 was detected by Western-bolt and RT-PCR. Results In the NPE 24 h and NPE 48 h groups,the growth rate of PMECs was significantly decreased with increased doubling time and a large amount of floating cell debris. In the simvastatin 24 h and simvastatin 48 h groups,the number of PMECs increased significantly. The expressions of Bax was sig-nificantly upregulated (P<0. 05),while the expressions of Bcl-2 was significantly downregulated (P<0. 05) in the NPE 24 h and NPE 48 h groups when compared with that in the control group. The expressions of Bax was significantly downregu-lated (P<0. 05),while the expressions of Bcl-2 was significantly upregulated (P<0. 05) in the simvastatin 24 h and sim-vastatin 48 h groups when compared with that in the control group. The expression of Bcl-2 of PMECs in the simvastatin 48 h group was significantly increased (P<0. 05) compared to the simvastatin 24 h group. Conlusions Simvastatin im-proves the growth of PMECs incubated with serum of NPE rat. One of the corresponding mechanisms may be related to the upregulation of Bcl-2 and downregulation of Bax.
