CAJ | 학술논문

目的：研究瓜蒌皮提取物(EPT)对大鼠血管内皮功能损伤的影响，为下一步探明瓜蒌皮保护血管内皮的有效组份做准备。方法用水煎醇沉法和萃取法提取瓜蒌皮的有效组份，用不同有效组份喂养低密度脂蛋白(LDL)损伤血管内皮大鼠，并检测血液内源性相关物质。结果单次静注LDL(4 mg/kg)显著诱导血管内皮细胞损伤，增加血浆中非对称二甲基精氨酸(ADMA)水平，而降低一氧化氮(NO)水平。两种提取(70%乙醇提取和氯仿萃取)的瓜蒌皮提取物均能显著抑制LDL所致ADMA浓度升高和NO的降低。结论瓜蒌皮70%醇提取再氯仿萃取物能明显减轻血管内皮损伤，对血管有明显的保护作用。
목적：연구과루피제취물(EPT)대대서혈관내피공능손상적영향，위하일보탐명과루피보호혈관내피적유효조빈주준비。방법용수전순침법화췌취법제취과루피적유효조빈，용불동유효조빈위양저밀도지단백(LDL)손상혈관내피대서，병검측혈액내원성상관물질。결과단차정주LDL(4 mg/kg)현저유도혈관내피세포손상，증가혈장중비대칭이갑기정안산(ADMA)수평，이강저일양화담(NO)수평。량충제취(70%을순제취화록방췌취)적과루피제취물균능현저억제LDL소치ADMA농도승고화NO적강저。결론과루피70%순제취재록방췌취물능명현감경혈관내피손상，대혈관유명현적보호작용。
Objective To study the effect of extractive of pericarpium trichosanthis( EPT) on damages of the endo-thelium induced by low-density lipoprotein (LDL),and find out the effective components of pericarpium trichosanthis in protecting endo-thelial cells. Methods Extract the effective component of pericarpium trichosanthis with extraction method of water decoction alcohol and precipitation. Use different effective components to feed rats of the endothelium of damages induced by LDL,and detect endogenous related substance. Results A single injection of LDL (4 mg/kg) sig-nificantly increased the serum levels of asymmetric dimethylarginine ( ADMA ) , reduced the serum levels of nitric oxide (NO). EPT with the different abstraction (70%alcohol and chloroform) markedly reduced the level of ADMA,,and in-creased the level of NO. Conclusion EPT with the different abstraction (70%alcohol and chloroform) protects the en-dothelium against damages induced by LDL.