CAJ | 학술논문

目的分析中国吉林省男性染色体多态与男性精液质量的相关性.方法选取2008至2013年吉林大学第一医院生殖医学中心就诊的2 584例不育患者(不育组)行体格检查、生殖激素检查、精液分析、染色体核型分析以及Y染色体微缺失检测,同时选取602名健康男性、50名核型正常的正常生育男性、50例核型正常的无精症患者以及50例核型正常的少精症患者分别作为对照组,进行比较分析.结果染色体多态在不育组与健康男性对照组中的总体发生率差异无统计学意义[3.91％(101/2 584)比3.16％ (19/602),P＞0.05].各常染色体多态在不育组与健康男性对照组中的发生率差异无统计学意义(均P ＞0.05).不育组患者中,小Y染色体(Yqh-)的发生率随患者精子浓度的降低而升高,其在无精症患者中的发生率显著高于少精症患者与精子数目正常者[57.14％(21/42)比24.32％(9/37)、0(0/13),均P＜0.05].不育组中伴染色体多态的无精症、少精症患者与核型正常的无精症、少精症对照组患者比较,睾丸大小、生殖激素水平差异均无统计学意义(均P＞0.05).PCR扩增结果表明,32.14％ (9/28)的Y染色体变异(Yqh±)患者存在Y染色体微缺失.结论常染色体多态与男性不育无明显相关性.但Y染色体多态可能是导致男性不育和Y染色体微缺失的重要因素.
목적 분석중국길림성남성염색체다태여남성정액질량적상관성.방법 선취2008지2013년길림대학제일의원생식의학중심취진적2 584례불육환자(불육조)행체격검사、생식격소검사、정액분석、염색체핵형분석이급Y염색체미결실검측,동시선취602명건강남성、50명핵형정상적정상생육남성、50례핵형정상적무정증환자이급50례핵형정상적소정증환자분별작위대조조,진행비교분석.결과 염색체다태재불육조여건강남성대조조중적총체발생솔차이무통계학의의[3.91％(101/2 584)비3.16％ (19/602),P＞0.05].각상염색체다태재불육조여건강남성대조조중적발생솔차이무통계학의의(균P ＞0.05).불육조환자중,소Y염색체(Yqh-)적발생솔수환자정자농도적강저이승고,기재무정증환자중적발생솔현저고우소정증환자여정자수목정상자[57.14％(21/42)비24.32％(9/37)、0(0/13),균P＜0.05].불육조중반염색체다태적무정증、소정증환자여핵형정상적무정증、소정증대조조환자비교,고환대소、생식격소수평차이균무통계학의의(균P＞0.05).PCR확증결과표명,32.14％ (9/28)적Y염색체변이(Yqh±)환자존재Y염색체미결실.결론 상염색체다태여남성불육무명현상관성.단Y염색체다태가능시도치남성불육화Y염색체미결실적중요인소.
Objective To evaluate the correlation between chromosomal polymorphisms and male sperm quality in Jilin Province.Methods A total of 2 584 male patients with infertility in Center for Reproductive Medicine,First Bethune Hospital of Jilin University from 2008 to 2013 were enrolled,which semen analysis,chromosomal analysis,Y chromosome microdeletion analysis and serum hormone levels analysis were performed.A total of 602 healthy individuals,50 fertile individuals with normal kayrotypes,50 azoospermia patients with normal kayrotypes and 50 oligospermia patients with normal kayrotypes were selected as control groups.Results There was no significant difference in the frequency of chromosome polymorphisms between infertile patients and normal control individuals (3.91％ (101/2 584)vs 3.16％ (19/602),P ＞ 0.05).And there was no significant difference in the frequency of autosomal polymorphisms between infertile patients and normal control individuals(all P ＞ 0.05).The frequency of Yqh-variant was increased by the decrease of sperm count and it appeared a significantly high frequency in azoospermia patients compared with oligospermia patients and sperm count normal patients in the infertile group (57.14％ (21/42) vs 24.32％ (9/37),0 (0/13),both P ＜ 0.05).There was no significant difference in the testis volume and serum hormone levels between the infertile patients with chromosomal polymorphisms and patients in control groups with normal kayrotypes (all P ＞ 0.05).The results of PCR amplication indicated that 32.14％ (9/28) patients with Yqh ± had Y chromosome microdeletion.Conclusions There is no significant correlation between autosomal polymorphisms and male infertility.But Yqh ± may be responsible for Y chromosome microdeletion and male infertility.