CAJ | 학술논문

以大理州不同居群滇龙胆(Gentiana rigescens Franch.ex Hemsl.)、同一居群不同植株为试验材料，采用高效液相色谱法(HPLC)对滇龙胆根及根茎中龙胆苦苷的含量进行测定；再以龙胆苦苷含量≥7.0％的优质种质资源为外植体，采用正交试验设计方法研究培养基、蔗糖浓度、pH及培养温度对滇龙胆离体培养植株再生的影响。结果表明，大理州6个居群滇龙胆根及根茎中的龙胆苦苷含量较高，不同居群间龙胆苦苷含量具有显著差异(P﹤0.05)，以鹤庆县居群的龙胆苦苷含量最高(80.70 mg/g)，含量最低的巍山县居群也超过国家药典规定标准的2倍多；同一居群内不同植株滇龙胆根及根茎中龙胆苦苷含量差异较小，只有云龙县居群不同植株间具有显著差异(P﹤0.05)，其他居群不同植株间无显著差异(P﹥0.05)；滇龙胆生长点离体培养及植株再生最适培养基和培养条件是1/2MS+30 g/L蔗糖+pH 5.2+20℃/25℃变温培养，再生植株诱导率最高可达88.9％，试验初步建立了滇龙胆离体培养及植株再生体系。
이대리주불동거군전룡담(Gentiana rigescens Franch.ex Hemsl.)、동일거군불동식주위시험재료，채용고효액상색보법(HPLC)대전룡담근급근경중룡담고감적함량진행측정；재이룡담고감함량≥7.0％적우질충질자원위외식체，채용정교시험설계방법연구배양기、자당농도、pH급배양온도대전룡담리체배양식주재생적영향。결과표명，대리주6개거군전룡담근급근경중적룡담고감함량교고，불동거군간룡담고감함량구유현저차이(P﹤0.05)，이학경현거군적룡담고감함량최고(80.70 mg/g)，함량최저적외산현거군야초과국가약전규정표준적2배다；동일거군내불동식주전룡담근급근경중룡담고감함량차이교소，지유운룡현거군불동식주간구유현저차이(P﹤0.05)，기타거군불동식주간무현저차이(P﹥0.05)；전룡담생장점리체배양급식주재생최괄배양기화배양조건시1/2MS+30 g/L자당+pH 5.2+20℃/25℃변온배양，재생식주유도솔최고가체88.9％，시험초보건립료전룡담리체배양급식주재생체계。
Gentiana rigescens Franch.ex Hemsl. plants from different populations and different individuals from the same population in Dali prefecture were selected as the experimental material. The gentiopicroside content of root and rhizome of G. rigescens was determined by High performance liquid chromatography (HPLC). Then the high quality germplasm resources of which the gentiopicroside content was not lower than 7.0% were selected as the explants; and the effects of culture medium, sucrose concentration, pH value, and culture temperature on the in vitro culture and plant regeneration of G. rigescens were explored by orthogonal experiment design. The experimental results showed that the gentiopicroside content of root and rhizome of G. rigescens from 6 different populations of Dali prefecture was high, and there was significant difference among different populations in the gentiopicroside content (P﹤0.05). Among them, the gentiopicroside content from Heqing county population was the highest (80.70 mg/g); and from Weishan county population was the lowest, which was still more than twice of the standard gentiopicroside content of national pharmacopoeia. The difference of the gentiopicroside content of root and rhizome of G . rigescens from the same population was smaller. There was significant difference in the gentiopicroside content among different plants from Yunlong county population (P﹤0.05), while no significant difference in other populations (P﹥0.05). The optimal culture medium and culture conditions for the growing point of in vitro culture and plant regeneration of G. rigescens of Dali populations was 1/2 MS + 30 g/L sucrose + pH 5.2, and variable temperature culture at 20℃/25℃. Under the condition, the regenerated plant inductivity was the highest as the inductivity reached to 88.9%. This study preliminary construct the in vitro culture and plant regeneration system for G. rigescens from Dali population.