医药导报
醫藥導報
의약도보
Herald of Medicine
2015年
10期
1363-1366
,共4页
程佩佩%夏叶%方玉%答国政%黄静%张秀桥
程珮珮%夏葉%方玉%答國政%黃靜%張秀橋
정패패%하협%방옥%답국정%황정%장수교
马蓝%靛蓝%靛玉红%含量测定%色谱法,高效液相
馬藍%靛藍%靛玉紅%含量測定%色譜法,高效液相
마람%전람%전옥홍%함량측정%색보법,고효액상
Baphicacanthus cusia%Indigo%Indirubin%Content determination%Chromatography,high performance liquid
目的：建立反相高效液相色谱( RP-HPLC)法同时测定不同产地、不同药用部位马蓝中靛蓝、靛玉红的含量。方法选用 Agilent TC-C18色谱柱(4.6 mm ×250 mm，5μm )，流动相为甲醇-水(75??25)，柱温25℃，流速1.0 mL??min－1，检测波长290 nm。结果靛蓝在0.0513~0.8208μg范围内与峰面积呈良好的线性关系(r＝0.9993)，平均回收率为99.00％，RSD为1.30％(n＝6)。靛玉红在0.0495~0.7920μg范围内与峰面积呈良好的线性关系(r＝0.9999)，平均回收率为98.88％，RSD为1.51％( n ＝6)。结论马蓝中靛蓝、靛玉红因产地和药用部位的不同，含量差异较大。该方法操作简便、快速、可靠，可为马蓝药材的质量控制提供实验依据。
目的：建立反相高效液相色譜( RP-HPLC)法同時測定不同產地、不同藥用部位馬藍中靛藍、靛玉紅的含量。方法選用 Agilent TC-C18色譜柱(4.6 mm ×250 mm，5μm )，流動相為甲醇-水(75??25)，柱溫25℃，流速1.0 mL??min－1，檢測波長290 nm。結果靛藍在0.0513~0.8208μg範圍內與峰麵積呈良好的線性關繫(r＝0.9993)，平均迴收率為99.00％，RSD為1.30％(n＝6)。靛玉紅在0.0495~0.7920μg範圍內與峰麵積呈良好的線性關繫(r＝0.9999)，平均迴收率為98.88％，RSD為1.51％( n ＝6)。結論馬藍中靛藍、靛玉紅因產地和藥用部位的不同，含量差異較大。該方法操作簡便、快速、可靠，可為馬藍藥材的質量控製提供實驗依據。
목적：건립반상고효액상색보( RP-HPLC)법동시측정불동산지、불동약용부위마람중전람、전옥홍적함량。방법선용 Agilent TC-C18색보주(4.6 mm ×250 mm，5μm )，류동상위갑순-수(75??25)，주온25℃，류속1.0 mL??min－1，검측파장290 nm。결과전람재0.0513~0.8208μg범위내여봉면적정량호적선성관계(r＝0.9993)，평균회수솔위99.00％，RSD위1.30％(n＝6)。전옥홍재0.0495~0.7920μg범위내여봉면적정량호적선성관계(r＝0.9999)，평균회수솔위98.88％，RSD위1.51％( n ＝6)。결론마람중전람、전옥홍인산지화약용부위적불동，함량차이교대。해방법조작간편、쾌속、가고，가위마람약재적질량공제제공실험의거。
Objective To establish a RP-HPLC method for determining indigo and indirubin in Baphicacanthus cusia from different producing areas and medicinal parts. Methods The separation was achieved by an Agilent TC-C18 Column (4.6 mm×250 mm, 5 μm) at 25 ℃ using methanol-water (75??25) as mobile phase at a flow rate of 1 mL??min-1.The detection wavelength was 290 nm. Results Indigo had a good linear relationship with peak area at range of 0. 051 3-0.820 8 μg (r=0.999 3).The recovery rate was 99.00% and RSD was 1.30% (n=6).Indirubin had a good linear relationship with peak area at range of 0.049 5-0.792 0 μg (r=0.999 9).The recovery rate was 98.88% and RSD was 1.51% (n=6). Conclusion The contents of the two components are obviously different in Baphicacanthus cusia because of different places or medicinal parts. The proposed method is simple, rapid and reliable. This method for determination of indigo and indirubin in Baphicacanthus cusia by RP-HPLC provides a basis for quality control of Baphicacanthus cusia.
