CAJ | 학술논문

目的研究二烯丙基二硫( DADS)抑制人胃癌MGC803细胞EMT与形态学变化。方法应用相差显微镜与透射电镜观察30 mg·L-1 DADS处理后的MGC803细胞形态学与超微结构的变化。 RT-PCR与West-ern blot检测β-catenin和EMT标记E-cadherin与Vimentin表达。结果30 mg · L-1 DADS处理MGC803细胞24 h后,相差显微镜显示,与未处理比较,细胞圆形、椭圆形,核浆比值降低,巢状分布,均未见伪足。透射电镜显示,细胞表面微绒毛数目减少,细胞核变规整,核浆比值下降,异型性降低,细胞与细胞之间出现连接结构,细胞器增多。 RT-PCR与Western blot表明,DADS可呈时间依赖性分别下调MGC803细胞β-catenin和Vimentin mRNA与蛋白和上调E-cadherin mRNA与蛋白。结论 DADS可通过下调β-catenin上调E-cadherin与下调Vimentin抑制EMT和伪足形成。
목적연구이희병기이류( DADS)억제인위암MGC803세포EMT여형태학변화。방법응용상차현미경여투사전경관찰30 mg·L-1 DADS처리후적MGC803세포형태학여초미결구적변화。 RT-PCR여West-ern blot검측β-catenin화EMT표기E-cadherin여Vimentin표체。결과30 mg · L-1 DADS처리MGC803세포24 h후,상차현미경현시,여미처리비교,세포원형、타원형,핵장비치강저,소상분포,균미견위족。투사전경현시,세포표면미융모수목감소,세포핵변규정,핵장비치하강,이형성강저,세포여세포지간출현련접결구,세포기증다。 RT-PCR여Western blot표명,DADS가정시간의뢰성분별하조MGC803세포β-catenin화Vimentin mRNA여단백화상조E-cadherin mRNA여단백。결론 DADS가통과하조β-catenin상조E-cadherin여하조Vimentin억제EMT화위족형성。
Objective To investigate the effect of EMT and morphology change in human gastric cancer MGC803 cells induced by diallyl disulfide (DADS). Methods The phase contrast microscope and transmission electron micro-scope were employed to confirm the DADS-induced morphology change. The expression ofβ-catenin,E-cadherin and Vimen-tin was detected by RT-PCR and Western blot in MGC803 cells after treated by 30 mg·L-1 DADS. Results The phase contrast microscope show that MGC803 cells after 24 h treated by 30 mg·L-1 DADS were round or ellipse,enlarged cyto-plasm,descend nuclear/cytoplasmic ratio,exhibited nest and even not view pseudopodia. Transmission electron microscope results show,that the number of microvilli on cells surface decrease,malignance declining as cellular heteromorphism dimin-ish,nucleocytoplasmic proportion reduce, intercellular conjunctional structure appear and cellular organ increase in cyto-plasm after MGC803 cells treated by DADS to contrast untreated. RT-PCR and Western blot indicated that DADS may be down-regulation of β-catenin and Vimentin mRNA and protein up-regulation of E-cadherin mRNA and protein in time de-pendence in MGC803 cells. Conclusion All these suggested that DADS may inhibit EMT and pseudopodia via down-regulation of β-catenin to up-regulate E-cadherin and down-regulate Vimentin in MGC803 cells.