中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2015年
9期
953-957
,共5页
梅鑫%陈银生%张洁%陈忠平
梅鑫%陳銀生%張潔%陳忠平
매흠%진은생%장길%진충평
神经胶质瘤%胶质母细胞瘤%肿瘤干细胞%细胞分化
神經膠質瘤%膠質母細胞瘤%腫瘤榦細胞%細胞分化
신경효질류%효질모세포류%종류간세포%세포분화
Glioma%Glioblastoma%Neoplastic stem cells%Cell differentiation
目的 采用活细胞荧光成像技术连续观察胶质瘤干细胞向内皮细胞分化过程中多种分子标记物的变化.方法 研究运用活细胞荧光成像技术,对胶质瘤干细胞细胞株GSC-1的分化过程进行12 h动态观察.同时应用免疫荧光技术在三维培养0、2、6h行内皮细胞表面标记物CD34染色,并通过光镜观察GSC-1的形态学变化.结果 在三维培养下GSC-1细胞能形成管道样结构,部分细胞表达内皮细胞标记物CD34,CD34阳性细胞数量随分化时间增长而增加(P＜0.05),而细胞的干性逐渐消失.活细胞荧光成像观察到同一视野下CD34阳性细胞数随诱导分化的时间推移而增加;单个GSC-1细胞由CD34阴性细胞转变成为CD34阳性细胞的全过程.结论 活细胞荧光成像技术监测细胞分化过程得到与传统免疫荧光方法一致的结果,而且更直观、更精确,能长时间对单个细胞追踪观察.
目的 採用活細胞熒光成像技術連續觀察膠質瘤榦細胞嚮內皮細胞分化過程中多種分子標記物的變化.方法 研究運用活細胞熒光成像技術,對膠質瘤榦細胞細胞株GSC-1的分化過程進行12 h動態觀察.同時應用免疫熒光技術在三維培養0、2、6h行內皮細胞錶麵標記物CD34染色,併通過光鏡觀察GSC-1的形態學變化.結果 在三維培養下GSC-1細胞能形成管道樣結構,部分細胞錶達內皮細胞標記物CD34,CD34暘性細胞數量隨分化時間增長而增加(P＜0.05),而細胞的榦性逐漸消失.活細胞熒光成像觀察到同一視野下CD34暘性細胞數隨誘導分化的時間推移而增加;單箇GSC-1細胞由CD34陰性細胞轉變成為CD34暘性細胞的全過程.結論 活細胞熒光成像技術鑑測細胞分化過程得到與傳統免疫熒光方法一緻的結果,而且更直觀、更精確,能長時間對單箇細胞追蹤觀察.
목적 채용활세포형광성상기술련속관찰효질류간세포향내피세포분화과정중다충분자표기물적변화.방법 연구운용활세포형광성상기술,대효질류간세포세포주GSC-1적분화과정진행12 h동태관찰.동시응용면역형광기술재삼유배양0、2、6h행내피세포표면표기물CD34염색,병통과광경관찰GSC-1적형태학변화.결과 재삼유배양하GSC-1세포능형성관도양결구,부분세포표체내피세포표기물CD34,CD34양성세포수량수분화시간증장이증가(P＜0.05),이세포적간성축점소실.활세포형광성상관찰도동일시야하CD34양성세포수수유도분화적시간추이이증가;단개GSC-1세포유CD34음성세포전변성위CD34양성세포적전과정.결론 활세포형광성상기술감측세포분화과정득도여전통면역형광방법일치적결과,이차경직관、경정학,능장시간대단개세포추종관찰.
Objective To observe the changes in a variety of molecular markers in the differentiation process from glioma stem cells to endothelial cells using live cell fluorescence imaging technique.Methods Live cell fluorescence imaging technique was used to study the differentiation process of glioma stem cell GSC-1 for dynamic observation of 12 h.The immunofluorescence staining was used to perform endothelial cell surface marker CD34 staining during the three dimensional culture at 0,2,and 6 h,and the morphological changes of GSC-1 were observed by light microscopy.Results Under the three-dimensional culture,GSC-1 cells could form tube-like structures.The number of some cells expressed endothelial cell markers CD34,CD34 positive cells increased with the time of differentiation (P ＜ 0.05),while stem marker disappeared gradually.Live cell fluorescence imaging observed that the number of CD34 positive cells increased with the time of induction and differentiation under the same field of vision.The whole process of a single GSC-1 cell transformed from a CD34 negative cell to a CD34 positive cell was observed.Conclusions The live cell fluorescence imaging technique detecting the cell differentiation process can get the same result of the traditional immunofluorescence assay,and it is more intuitive and accurate.It can follow up and observe a single cell for a long time.
